A5077300458- Metabolomics and Mass Spectrometry Studies
- GDF15 and Related Biomarkers
- Radiopharmaceutical Chemistry and Applications
- Alzheimer's disease research and treatments
- Dialysis and Renal Disease Management
- Analytical Chemistry and Chromatography
- Crystallization and Solubility Studies
- Iron Metabolism and Disorders
- Macrophage Migration Inhibitory Factor
- Analytical Methods in Pharmaceuticals
- Monoclonal and Polyclonal Antibodies Research
- Genomics, phytochemicals, and oxidative stress
- X-ray Diffraction in Crystallography
- Vasculitis and related conditions
- Edible Oils Quality and Analysis
- Drug Solubulity and Delivery Systems
- Bee Products Chemical Analysis
- Mechanisms of cancer metastasis
- Peptidase Inhibition and Analysis
- Phytochemicals and Antioxidant Activities
- Biochemical and Structural Characterization
- Spectroscopy and Chemometric Analyses
- Curcumin's Biomedical Applications
- Nuclear Receptors and Signaling
- Cellular Mechanics and Interactions
National and Kapodistrian University of Athens
2015-2024
National Centre of Scientific Research "Demokritos"
2004-2015
Siderophores have mostly been studied concerning their contribution to the fitness and virulence of bacterial pathogens. In present work, we isolated characterized for first time siderophore bacillibactin from a commercial biocontrol agent.
The synthesis and characterization of “2 + 1” complexes the [M(CO)3]+ (M = Re, 99mTc) core with β-diketones acetylacetone (complexes 2, 8) curcumin 5, 10 6, 11) as bidentate OO ligands, imidazole or isocyanocyclohexane monodentate ligands is reported. were synthesized by reacting [NEt4]2[Re(CO)3Br3] precursor β-diketone to generate intermediate aqua complex fac-Re(CO)3(OO)(H2O) that was isolated characterized, followed replacement labile water ligand. All characterized mass spectrometry, NMR...
Abstract Metazoans viability depends on their ability to regulate metabolic processes and also respond harmful challenges by mounting anti‐stress responses; these adaptations were fundamental forces during evolution. Central responses are a number of short‐lived transcription factors that functioning as stress sensors mobilize genomic aiming eliminate stressors. We show here increased expression nuclear factor erythroid 2‐related (Nrf2) in Drosophila activated cytoprotective modules enhanced...
Abstract Aim Recent evidence suggests that arterial hypertension could be alternatively explained as a physiological adaptation response to water shortage, termed aestivation, which relies on complex multi‐organ metabolic adjustments prevent dehydration. Here, we tested the hypothesis chronic loss across diseased skin leads similar adaptive conservation responses observed in experimental renal failure or high salt diet. Methods We studied mice with keratinocyte‐specific overexpression of...
Abstract Although late-onset sepsis (LOS) is a major cause of neonatal morbidity and mortality, biomarkers evaluated in LOS lack high diagnostic accuracy. In this prospective, case-control, pilot study, we aimed to determine the metabolic profile neonates with LOS. Urine samples were collected at day initial evaluation, 3 rd 10 th day, thereafter, from 16 septic (9 confirmed 7 possible cases) non-septic ones (controls) respective time points. profiles assessed using non-targeted nuclear...
Vaccination is currently the most effective strategy for mitigation of COVID-19 pandemic. mRNA vaccines trigger immune system to produce neutralizing antibodies (NAbs) against SARS-CoV-2 spike proteins. However, underlying molecular processes affecting response after vaccination remain poorly understood, while there significant heterogeneity in among individuals. Metabolomics have often been used provide a deeper understanding cell responses, but context such data are scarce. Mass...
The in vitro utilization of biocompatible ferromagnetic nanoparticles (BFNs) hemodialysis (HD), routinely used today for the treatment end stage renal disease (ESRD), is introduced this work. proposed strategy termed magnetically assisted (MAHD) and it aims to become a more efficient development conventional HD. method based on production nanoparticles-targeted binding substances conjugates (BFNs-TBSs Cs) constructed BFNs specifically designed TBSs that should have high affinity capacity...
Abstract The discovery of new active natural products is hampered by laborious purification processes that often end up to the re‐isolation known compounds. We demonstrate here that, spectral data reflecting concentration fluctuations components can correlate statistically with measurable dose‐dependent properties on basis a Heterocovariance approach deconvoluting structure. Variance extract constituents was achieved through meaningful collections plants from different families, genus, and...
Melanoma is the most aggressive type of skin cancer, leading to metabolic rewiring and enhancement metastatic transformation. Efforts improve its early accurate diagnosis are largely based on preclinical models especially cell lines. Hence, we herein present a combinational Nuclear Magnetic Resonance (NMR)- Ultra High Performance Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (UHPLC-HRMS/MS)-mediated untargeted metabolomic profiling melanoma cells, landscape alterations...
Conventional isolation methods in natural products chemistry are time-consuming and costly often result the of moderately active compounds or detection already known (NPs). A fast cost-effective way to identify bioactive metabolites plant extracts prior has been developed based on nuclear magnetic resonance (NMR)-heterocovariance approach (NMR-HetCA). In order evaluate depth application this chemometrics-based drug discovery methodology, simple mixtures 10 standard NPs simulating a...
Magnetically assisted hemodialysis is a development of conventional and based on the circulation ferromagnetic nanoparticle-targeted binding substance conjugates (FN-TBS Cs) in bloodstream patient their eventual removal by means 'magnetic dialyzer'. Presented here an vitro investigation biocompatibility bare Fe(3)O(4) FNs Fe(3)O(4)-bovine serum albumin Cs with blood cells, namely red cells (RBCs), white (WBCs) platelets (Plts). Atomic force microscopy (AFM) optical (OM) enabled examination...
Ischemic preconditioning, which is mediated by cell signaling molecules, protects the heart from ischemia-reperfusion injury limiting infarct size. Oleuropein, main polyphenolic constituent of olives, reduced size in normal and cholesterol-fed rabbits when it was administered at a nutritional dose. The aim present study to compare effects oleuropein preconditioning terms metabolism pathways underlying myocardial protection. Rabbits were randomly divided into six groups: control group...
Giant Cell Arteritis-(GCA) is an inflammatory disease following a chronic, relapsing course. The metabolic alterations related to the intense process during active phase and rapid impact of steroid treatment, remain unknown. study aims investigate serum metabolome in inactive state.110 samples from 50 patients [33-GCA 17-Polymyalgia rheumatica-(PMR)] at 3 time points, 0-(V1: disease), 1 6 months-(V2 V3: remission) treatment with glucocorticosteroids (GCs), were subjected Nuclear Magnetic...
Prostate cancer is the second most commonly diagnosed in men worldwide, which almost incurable, once it progresses into metastatic stage. Adriamycin (ADR) a known chemotherapeutic agent that causes severe side effects. In recent years, studies natural plant products have revealed their anticancer activities. particular, Glycyrrhiza glabra enhanced extract (GGE), as licorice, has been reported to exert antiproliferative properties against cells. this study, cytotoxic potential of GGE was...
Silymarin-enriched extract (SEE) is obtained from Silybum marianum (Asteraceae). Doxorubicin (DXR) a widely used chemotherapeutical yet with severe side effects. The goal of the present study was to assess pharmacologic effect SEE and its bioactive components silibinin silychristine when administrated alone or in combination DXR human prostate cancer cells (PC-3). PC-3 were treated SEE, (silybins A B), silychristine, alone, DXR, cell proliferation assessed by MTT assay. Cell cycle,...
Iron oxide Ferromagnetic Nanoparticles (FNs) such as magnetite (Fe3O4) and maghemite (γ-Fe2O3) are currently employed in biomedical applications owing to their relatively high biocompatibility. Recently, we have introduced a novel application of Fe3O4 FNs the so-called Magnetically Assisted Haemodialysis (MAHD), promising concept that can be for treatment End-Stage Renal Disease. The key characteristic MAHD is selective removal toxins cannot removed by current low- high-flux dialysers...
The aim of the present study is evaluation 99mTc complexes two bombesin-like peptides: Gly1′-Gly2′-Cys3′-Aca-BN[2−14] (BN-1.1) and Gly1′-Gly2′-Cys3′-Aca-BN[7−14] (BN-1.1p). BN derivatives were synthesized according to solid phase peptide synthesis method, characterized by ESI-MS NMR. 185/187Re-BN-1.1 185/187Re-BN-1.1p also identified stable over time in human plasma, while they degraded rapidly kidney−liver homogenates. peptides their showed high affinity for GRP receptors expressed PC-3...