A5086180613- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Crystal structures of chemical compounds
- Metal complexes synthesis and properties
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- GDF15 and Related Biomarkers
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- T-cell and B-cell Immunology
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Cancer Mechanisms and Therapy
- Magnetism in coordination complexes
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Chemical synthesis and pharmacological studies
- Protein Kinase Regulation and GTPase Signaling
- Synthesis and Characterization of Heterocyclic Compounds
- Bone Metabolism and Diseases
- CRISPR and Genetic Engineering
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Glycosylation and Glycoproteins Research
National Centre of Scientific Research "Demokritos"
2008-2024
Institute of Biosciences & Applications
2014-2024
University of Crete
1993-2006
Democritus University of Thrace
2006
UCLouvain
1989
Gromita is a fully integrated and efficient graphical user interface (GUI) to the recently updated molecular dynamics suite Gromacs, version 4. cross-platform, perl/tcl-tk based, interactive front end designed break command line barrier introduce new user-friendly environment run simulations through Gromacs. Our GUI features novel workflow that guides each logical step of setup process, making it accessible both advanced novice users. This tool provides seamless Gromacs package, while...
Tuberous sclerosis complex (TSC) is a rare autosomal dominant disorder causing benign tumors in the brain and other vital organs. The genes implicated disease development are TSC1 TSC2. Here, we have performed mutational analysis followed by genotype-phenotype correlation study based on clinical characteristics of affected individuals. Twenty unrelated probands or families from Greece been analyzed, whom 13 had definite TSC, whereas another 7 possible TSC diagnosis. Using direct sequencing,...
The crystal structures of bovine pancreatic ribonuclease A (RNase A) in complex with 3',5'-ADP, 2',5'-ADP, 5'-ADP, U-2'-p and U-3'-p have been determined at high resolution. reveal that each inhibitor binds differently the RNase active site by anchoring a phosphate group subsite P1. most potent all five, 5'-ADP (Ki = 1.2 microM), adopts syn conformation (in contrast to 3',5'-ADP which adopt an anti), it is beta- rather than alpha-phosphate 5'-phosphate P1 adenosine B2 pocket. Two different...
Poly(A)-specific ribonuclease (PARN) is a cap-interacting deadenylase that mediates, together with other exonucleases, the eukaryotic mRNA turnover and thus actively involved in regulation of gene expression. Aminoglycosides natural nucleotides are only reported modulators human PARN activity, so far. In present study, we show synthetic nucleoside analogues bearing fluoro-glucopyranosyl sugar moiety benzoyl-modified cytosine or adenine as base can effectively inhibit PARN. Such exhibited...
Serine/arginine protein kinases (SRPKs) phosphorylate Arg/Ser dipeptide-containing proteins that play crucial roles in a broad spectrum of basic cellular processes. The existence large internal spacer sequence separates the bipartite kinase catalytic core is unique structural feature SRPKs. Previous studies on catalytically active fragment SRPK1, which lacks main part domain, revealed SRPK1 remains an state without any post-translational modifications or specific intra-protein interactions,...
Activated Akt has been previously implicated in acting on RS domain-containing proteins. However, it questioned whether its action is direct or mediated by co-existing SR kinase activity. To address this issue we studied detail the phosphorylation of Lamin B Receptor (LBR) Akt. Using synthetic peptides and a set recombinant proteins expressing mutants LBR domain now demonstrate that while all serines represent more less equal phosphoacceptor sites for SRPK1, Ser80 Ser82 are mainly targeted...
The tyrosine kinase 2 protein (Tyk2), encoded by the TYK2 gene, has a crucial role in signal transduction and pathogenesis of many diseases. A single nucleotide polymorphism SNP rs34536443, is major importance, since it been shown to confer protection against various, mainly, autoimmune This results Pro Ala change at amino acid position 1104 Tyk2 that affects its enzymatic activity. However, details underlined mechanism are unknown. To address this issue, study, we used molecular dynamics...
Potentiometric investigations in aqueous solution at 25 °C and ionic strength 0.1 mol dm–3 KCl have shown that the dimer complex species [Cu2L2]4–(ligand H4L, m-phenylenediamine-N,N,N,′N′-tetra-acetic acid, m-H4pdta) [Ni2L2]4–(ligand pyridine-2,6-diamine-N,N,N′,N′-tetra-acetic 2,6-H4pydta) are present significant amounts CM > 2 × 10–3 dm–3. The formation constants of monomer been determined. [M2L2]4– from [ML]2– or [M(HL)]– is affected by Jahn–Teller effect (CuII–m-H4pdta) withdrawal...
Ssn6, a yeast protein that comprises 10 tandem tetratricopeptide repeat (TPR) motifs, associates with Tup1 repressor and acts as transcriptional corepressor. In this report we identify point mutations in the TPR1 of Ssn6 disrupt interaction. Furthermore, construct 3D model TPR domain which is responsible for binding, based on known structure phosphatase 5. According to all selected reduce ability interact by affecting structural integrity and/or correct spatial arrangement relative TPR2 TPR3.
The repressor of primer (Rop) protein has become a steady source surprises concerning the relationship between sequences and structures several its mutants variants. Here we add another piece to puzzle Rop by showing that an engineered deletion mutant (corresponding residues 30-34 wild-type designed restore heptad periodicity at turn region) results in complete reorganization bundle which is converted from homodimer homotetramer. In contrast (and as previously shown), two-residue insertion,...
Abstract Ssn6, a tetratricopeptide repeat (TPR) containing protein, associates with the Tup1 repressor to form global transcriptional co‐repressor complex, which is conserved across species. The three N‐terminal TPR repeats of out total 10, are involved in this particular interaction. Our previously reported 3D‐modeling and mutagenesis data suggested that structural integrity TPR1 its correct positioning relatively TPR2 crucial for binding. In study, we first investigate stability binding...
The human mineralocorticoid receptor (MR) is one of the main components renin-angiotensin-aldosterone system (RAAS), that regulates body exchange water and sodium. evolutionary origins this protein predate those renin RAAS; accordingly it has other roles, which are being characterized. MR two trans-activating ligand independent domains inhibitory domain (ID), modulates activity former. structure ID currently unknown.Here we report contains at least 15 tandem repeats around 10 amino acids,...