Three efficient inorganic-phosphate solubilizing bacteria (PSB) were isolated from a phosphate rock deposit of Moroccan mine. The solubilization index these isolates, determined in National Botanical Research Institute's (NBRIP) medium supplemented with tribasic calcium phosphate, ranging 2.8 to 4.4. pH dropped 7.0 3.5 units after growth under continuous agitation for seven days. PSB6, the most PSB, closely related

Dense granules, a type of platelet secretory organelle, are known to accumulate high concentrations small molecules such as calcium, adenine nucleotides, serotonin, pyrophosphate, and polyphosphate. Protein composition these granules has been obscure, however. In this paper, we use proteomics techniques describe, for the first time, soluble protein dense granules. We have isolated highly enriched human granule fractions that analyzed using two methods. Using approach, identified 40 proteins,...

Summary Costa Rica is at the centre of Mesoamerican biodiversity hotspot. Little known about cyanobacteria from this region so far. Here, four isolates order Stigonematales (section V) were characterized in a polyphasic approach. All strains isolated geothermal sites and hot springs Rica. However, one them, identified as Westiellopsis sp. Ar73, did not grow more than 40°C. Based on its identical 16S rRNA to several previously Fischerella muscicola strains, ubiquitous distribution throughout...

Fluorine accounts for 0.3 g/kg of the Earth’s crust, being widely distributed in environment as fluoride. The toxic effects this anion humans and other organisms have been known a long time. Fluoride has reported to alter several cellular processes although mechanisms involved are largely unknown. Inorganic pyrophosphatases (PPases) ubiquitous enzymes that hydrolyze inorganic pyrophosphate (PPi), metabolite generated from ATP. In Saccharomyces cerevisiae, enzyme responsible PPi hydrolysis...

An NADPH-glutathione reductase (EC 1.6.4.2) has been purified 6,000-fold to electrophoretic homogeneity from the filamentous cyanobacterium Anabaena sp. strain 7119. The enzyme exhibits a specific activity of 249 U/mg and is characterized by being dimeric flavin adenine dinucleotide-containing protein with ratio absorbance at 280 nm 462 5.8, native molecular weight 104,000, Stokes radius 4.13 nm, pI 4.02. inhibited sulfhydryl reagents heavy-metal ions, especially in presence NADPH, oxidized...

Bats are emblematic hosts of caves. These small flying mammals deserve special attention because their presence has a great economic and ecological impact; they introduce organic matter, the guano, in ecosystem live in. Indeed, “guano” (a Quechua word meaning “fertilizer”) is accumulation fecal matter (excreta); its deposition can reach several meters. The composition guano influenced by bat’s food. In addition to role within caves, bat exploited for various purposes; it used as an effective...

Two types of proteins that hydrolyze inorganic pyrophosphate (PP i ), very different in both amino acid sequence and structure, have been characterized to date: soluble membrane-bound proton-pumping pyrophosphatases (sPPases H + -PPases, respectively). sPPases are ubiquitous PP releasing heat, whereas so far unidentified animal fungal cells, couple the energy hydrolysis proton movement across biological membranes. The budding yeast Saccharomyces cerevisiae has two located cytosol...

ABSTRACT Soluble and membrane-bound inorganic pyrophosphatases (sPPase H + -PPase, respectively) of the purple nonsulfur bacterium Rhodospirillum rubrum are differentially regulated by environmental growth conditions. Both proteins their transcripts were found in cells anaerobic phototrophic batch cultures along all phases, although they displayed different time patterns. However, aerobic that grow dark, which exhibited highest rates, Northern Western blot analyses as well activity assays...

Current evidence suggests the occurrence of two classes vacuolar‐type H + ‐translocating inorganic pyrophosphatases (V‐PPases): K ‐insensitive proteins, identified in eukaryotes, bacteria and archaea, ‐stimulated V‐PPases, to date only eukaryotes. Here, we describe functional characterization a thermostable V‐PPase from anaerobic hyperthermophilic bacterium Thermotoga maritima by heterologous expression Saccharomyces cerevisiae . The activity this 71‐kDa membrane‐embedded polypeptide has...

Two sPPases (soluble inorganic pyrophosphatases, EC 3.6.1.1) have been isolated from the microalga Chlamydomonas reinhardtii. Both are monomeric proteins of organellar localization, chloroplastic sPPase I [Cr (Ch. reinhardtii)-sPPase I, 30 kDa] is a major isoform and slightly larger protein than mitochondrial II (Cr-sPPase II, 24 kDa). They members family encoded by two different cDNAs, as demonstrated peptide mass fingerprint analysis. Molecular phylogenetic analyses indicated that...

V-ATPases (vacuolar H+-ATPases) are a specific class of multi-subunit pumps that play an essential role in the generation proton gradients across eukaryotic endomembranes. Another simpler pump co-localizes with V-ATPase occurs plants and many protists: single-subunit H+-PPase [H+-translocating PPase (inorganic pyrophosphatase)]. Little is known about relative contribution these two proteins to acidification intracellular compartments. In present study, we show expression chimaeric derivative...

A strain of the protozoan ciliate Tetrahymena thermophila adapted to increasing Pb(II) concentrations over two years has shown that one resistance mechanisms this extreme metal stress is lead biomineralization chloropyromorphite, most stable minerals in earth's crust. Several techniques such as microanalysis coupled transmission and scanning electron microscopy (X-Ray Energy Disperse Spectroscopy), fluorescence X-ray power diffraction analysis have revealed presence chloropyromorphite...