Two exopolysaccharides (EPS) producing strains, isolated from raw donkey milk were identified as Leuconostoc mesenteroides SL and Enterococcus viikkiensis N5 using 16S rDNA sequencing. The Box Benheken design exhibited the highest yield of EPS-SL (672.342 mg/L) produced by EPS-N5 (901 N5. molecular weight was 1.68×104 for 1.55×104 Da EPS-N5. FTIR, NMR GC-MS analysis showed that EPS are heteropolysaccharides. SEM image smooth represented a lotus leaf shape revealed stiff-like, porous...

Infectious diseases remain a major global health concern. Cistus ladanifer, plant commonly employed in Moroccan traditional medicine, has been identified as potential antiviral candidate. This study aimed to evaluate the activity of C. ladanifer extracts vitro and silico against two respiratory viruses: Herpes simplex virus severe acute syndrome coronavirus 2 variants concern Delta Omicron. Toxic prediction main compounds by Gas Chromatography‐Mass Spectrometry from was performed via...

In the current research, Enterococcus mundtii SRBG1 newly isolated from Bat guano was encapsulated using spray drying technique to create a probiotic powder six combinations of inulin, maltodextrin and sodium alginate. The encapsulation yield, moisture content, physical characteristics, shape were investigated. Microcapsules yields ranged 67 85 percent, which is consistent with typical B-290 spray-drier yields. content showed increase (4 ± 0.15%) addition alginate inulin maltodextrin....

Bats are emblematic hosts of caves. These small flying mammals deserve special attention because their presence has a great economic and ecological impact; they introduce organic matter, the guano, in ecosystem live in. Indeed, “guano” (a Quechua word meaning “fertilizer”) is accumulation fecal matter (excreta); its deposition can reach several meters. The composition guano influenced by bat’s food. In addition to role within caves, bat exploited for various purposes; it used as an effective...

Enterococci, known for their resilience, are commonly found in food, the environment, and gastrointestinal tracts of humans animals. In recent research, six strains enterococcus were isolated from bat guano. These include Enterococcus mundtii SRBG1, gallinarum SRBG3, faecium SRBG2, casseliflavus EC1, devriesei CAU 1344. Identification was done using 16S DNA analysis. Each strain underwent evaluation its technological properties (such as tolerances to various NaCl concentrations temperatures,...

Recently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is widely studied, but its microbiota, especially lactic acid bacteria, remains less studied. This study focuses on analyzing, isolating, identifying bacteria evaluating capacity to produce biomolecules with antibacterial activity. Among 44 strains identified, 43 are Gram-positive, most catalase-negative cocci-shaped. Five were...

The objective of this work is to study the improvement effect some essential oils sage ( Salvia officinalis ), oregano Origanum vulgare and eucalyptus globulus ) on physiological parameters characterizing quality human sperm (mobility vitality). We find natural biomolecules improve increase chances success very low in vitro fertilization (IVF) that stagnate around 20%. Sperm samples were mixed with different concentrations oils. these motility vitality spermatozoa has been analyzed. mobility...

The widespread global COVID-19 pandemic due to the lack of specific treatment and urgent situation requires use all resources remedy this scourge. current study aimed molecular docking tools find potential drug candidates for treatment. pyrano[2,3-c] pyrazole 5(a-e) was targeted against Main protease (Mpro), which plays a vital role in replication transcription Corona viral genome. 3CL Protease (PDB ID 6LU7) modeled, compounds were docked using Autodock Vina software, ADMET data have been...

Background: Antioxidants are developed to assist the immune system and overcome oxidative stress, aggression of cellular constituents due imbalance between reactive oxygen species inner antioxidant system. The main objective this study was search for new potent antioxidants protect humans against diseases associated with stress. Methods: In study, three pyrano-[2,3-c]-pyrazole derivatives were synthesized via Multicomponent Reaction (MCR) approach characterized, using a melting point,...

In this paper, we present experimental findings on the protective effect of some bis-benzimidazole molecules against oxidative (H 2 O ) and nitrosative (SNP) stress using protozoan T. thermophila as a cellular model. The results article show that 2,2’-Benzendiyl-1,4-Bis-(5-amino-1H-benzimidazole) 2,2’-Octandiyl-1,8-Bis-(quinolin-8-ol-5-azo-1H-benzimidazole) have important than molecule 2,2’-Benzendiyl-1,4-Bis-(1H-benzimidazole).

The hospital environment favors the circulation of drug-resistant bacteria. researcher has oriented this public health problem to find an ideal tool for better patient management. Therefore, study aims assess biological activity some synthetic molecules against multidrug-resistant bacterial isolates from patients suspected nosocomial infections. After synthesizing and characterizing five targeted compounds 5(a-e), a sensitivity test is carried out screen their antibacterial via...

Nowadays, cutting-edge Artificial intelligence systems demonstrate exceptional capabilities in comprehending, generating, and manipulate human language, ushering a new era of understanding, collaboration, innovation. This systematic review explores the varied applications ChatGPT within realm biomedicine patient care, aiming to uncover its potential transforming textual information into actionable insights for improved healthcare outcomes. To conduct this study, we followed PRISMA guidelines...

Male infertility is a complex multifactorial disease and real health problem; 50% of infertile men have identifiable causes detectable by fundamental sperm analysis. Numerous research studies shown the possibility treating abnormal semen samples with some drugs before artificial insemination, yet evidence drug's effectiveness remains minimal. In our previous work, we tested essential oils, such as eucalyptus (Eucalyptus globulus Labill.), oregano (Origanum vulgare L.), sage (Salvia...

Due to its similarity with human milk and low allergenic properties, donkey has long been used as an alternative for infants patients cow's protein allergy. In addition, this is attracting growing interest in nutrition because of presumed health benefits. It antioxidant, antimicrobial, antitumoral, antiproliferative, antidiabetic activity. Also, it stimulates the immune system, regulates gastrointestinal flora, prevents inflammatory diseases. Although all components can contribute functional...

This study aims to evaluate the antioxidant activity of pyranopyrazoles derivatives synthesized via multicomponent reaction and characterized by spectroscopic techniques ( 1 H-NMR; 13 C-NMR IR).All compounds were screened for their anti-stress properties in vivo using as a test organism eukaryotic cells "Tetrahymena" exposed oxidative nitrosative stress.Thereafter vitro is performed 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays.Our results clearly showed protective behavior studied...

The widespread of multi-resistant strains due to the lack specific treatment and propagation infectious diseases requires all resources remedy this scourge. This study is therefore aimed assess antibacterial activity four synthetic α-Aminophosphonate 4(a-d). Methods: Firstly, has been synthesized characterized, then molecular docking these compounds 4(a-d) into active binding site Escherichia coli MurB enzyme (PDB Id: 1MBT) was performed gain a comprehensive understanding their biological...