A5028106597- Diamond and Carbon-based Materials Research
- Force Microscopy Techniques and Applications
- Boron Compounds in Chemistry
- Carbon Nanotubes in Composites
- Metal and Thin Film Mechanics
- Crystallization and Solubility Studies
- Ion-surface interactions and analysis
- X-ray Diffraction in Crystallography
- Radiopharmaceutical Chemistry and Applications
- Nanocluster Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Nanomaterials in Catalysis
- Nanoparticle-Based Drug Delivery
- Boron and Carbon Nanomaterials Research
- Advanced biosensing and bioanalysis techniques
- Fullerene Chemistry and Applications
- RNA Interference and Gene Delivery
- Plant Micronutrient Interactions and Effects
- Analytical Chemistry and Sensors
- High-pressure geophysics and materials
- Graphene and Nanomaterials Applications
- Lanthanide and Transition Metal Complexes
- Nonlinear Optical Materials Studies
- Plant Stress Responses and Tolerance
- Enzyme function and inhibition
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2015-2024
Charles University
1999-2023
Istituto Nazionale di Ricerca Metrologica
2023
University of Turin
2023
Torino e-district
2023
Istituto Nazionale di Fisica Nucleare, Sezione di Torino
2023
Czech Academy of Sciences
2004-2022
University of Veterinary Medicine Vienna
2020
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2013
University of Chemistry and Technology, Prague
2003-2011
Cellular fate of nanoparticles is vital to application cell imaging, bio-sensing, drug delivery, suppression resistance, gene delivery and cytotoxicity analysis. However, the current studies on cellular have been controversial due complications interplay between many possible factors. By well-controlled experiments, we demonstrated unambiguously that morphology independently determined their fate. We found with sharp shapes, regardless surface chemistry, size, or composition, could pierce...
Photoluminescent nanosized allotropes of carbon have attracted considerable interest because their diverse optical properties depending on crystal structure, size, and morphology, chemical functionalization. Here, we present the first critical review covering photoluminescence (PL) properties, control, origin in various composites. Different mechanisms by which nanostructures exhibit PL are discussed, involving excitonic nanotubes, thermally activated delayed fluorescence spherical...
HIV protease (PR) represents a prime target for rational drug design, and inhibitors (PI) are powerful antiviral drugs. Most of the current PIs pseudopeptide compounds with limited bioavailability stability, their use is compromised by high costs, side effects, development resistant strains. In our search novel PI structures, we have identified group inorganic compounds, icosahedral metallacarboranes, as candidates class nonpeptidic PIs. Here, report potent, specific, selective competitive...
Abstract Development of multifunctional nanoscale sensors working under physiological conditions enables monitoring intracellular processes that are important for various biological and medical applications. By attaching paramagnetic gadolinium complexes to nanodiamonds (NDs) with nitrogen-vacancy (NV) centres through surface engineering, we developed a hybrid sensor can be adjusted directly monitor species proposed sensing scheme based on NV spin relaxometry. We adopt single-step method...
Abstract High biocompatibility, variable size ranging from ≈5 nm, stable luminescence its color centers, and simple carbon chemistry for biomolecule grafting make nanodiamond (ND) particles an attractive alternative to molecular dyes drug‐delivery. A novel method is presented that can be used remote monitoring of chemical processes in biological environments based on changes photoluminescent (PL) nitrogen‐vacancy (NV) centers ND. The NV driven chemically by alternating the surface potential...
HIV protease (HIV PR) is a primary target for anti-HIV drug design. We have previously identified and characterized substituted metallacarboranes as new class of inhibitors. In structure-guided design effort, we connected the two cobalt bis(dicarbollide) clusters with linker to ammonium group obtained set compounds based on lead formula [H2N-(8-(C2H4O)2-1,2-C2B9H10)(1′,2′-C2B9H11)-3,3′-Co)2]Na. explored inhibition properties these various substitutions, determined PR:inhibitor crystal...
Triphenylphosphonium cations strongly enhance the PL quantum yield of near infrared photoluminescent gold nanoclusters by metal-to-ligand charge transfer.
A novel approach for preparation of ultra-bright fluorescent nanodiamonds (fNDs) was developed and the thermal kinetic optimum NV center formation identified. Combined with a new oxidation method, this enabled particles that were roughly one order magnitude brighter than prepared commonly used procedures.
High pressure high temperature (HPHT) nanodiamonds (NDs) represent extremely promising materials for construction of fluorescent nanoprobes and nanosensors. However, some properties bare NDs limit their direct use in these applications: they precipitate biological solutions, only a limited set bio‐orthogonal conjugation techniques is available the accessible material greatly polydisperse shape. In this work, we encapsulate bright 30‐nm (FNDs) 10–20‐nm thick translucent (i.e., not altering...
Biocompatible nanoscale probes for sensitive detection of paramagnetic species and molecules associated with their (bio)chemical transformations would provide a desirable tool better understanding cellular redox processes. Here, we describe an analytical based on quantum sensing techniques. We magnetically coupled negatively charged nitrogen-vacancy (NV) centers in nanodiamonds (NDs) nitroxide radicals present bioinert polymer coating the NDs. demonstrated that T1 spin relaxation time NV is...
Free radicals are crucial indicators for stress and appear in all kinds of pathogenic conditions, including cancer, cardiovascular diseases, infection. However, they difficult to detect due their reactivity low abundance. We use relaxometry the detection with subcellular resolution. This method is based on a fluorescent defect diamond, which changes its optical properties basis magnetic surroundings. technique allows nanoscale MRI unprecedented sensitivity spatial Recently, this was used...
Temperature is one of the most relevant parameters for regulation intracellular processes. Measuring localized subcellular temperature gradients fundamental a deeper understanding cell function, such as genesis action potentials, and metabolism. Here, we detect first time variations (1{\deg}C) associated with potentiation depletion neuronal firing, exploiting nanoscale thermometer based on optically detected magnetic resonance in nanodiamonds. Our results provide tool assessing spiking...
CA inhibitors: Human carbonic anhydrases (CAs) are diagnostic and therapeutic targets. Various carborane cages shown to act as active-site-directed inhibitors, substitution with a sulfamide group other substituents leads compounds high selectivity towards the cancer-specific isozyme IX. Crystal structures of carboranes in active site provide information that can be applied structure-based design specific inhibitors. As service our authors readers, this journal provides supporting supplied by...
Core-shell nanoparticles based on fluorescent nanodiamonds coated with a biocompatible N-(2-hydroxypropyl)methacrylamide copolymer shell were developed for background-free near-infrared imaging of cancer cells. The particles showed excellent colloidal stability in buffers and culture media. After conjugation cyclic RGD peptide they selectively targeted integrin αvβ3 receptors glioblastoma cells high internalization efficacy.
This is the first experimental evidence that both self-assembly and surface activity are common features of all water-soluble boron cluster compounds. The solution behavior anionic polyhedral boranes (sodium decaborate, sodium dodecaborate, mercaptododecaborate), carboranes (potassium 1-carba-dodecaborate), metallacarboranes {sodium [cobalt bis(1,2-dicarbollide)]} was extensively studied, it evident clusters form multimolecular aggregates in water. However, mechanism aggregation dependent on...
Abstract Fluorescent nanodiamonds (FNDs) are vital to many emerging nanotechnological applications, from bioimaging and sensing quantum nanophotonics. Yet, understanding engineering the properties of fluorescent defects in remain challenging. The most comprehensive study date is presented, optical physical five different nanodiamond samples, which nitrogen‐vacancy (NV) centers created using fabrication techniques. FNDs' fluorescence spectra, lifetime, spin relaxation time ( T 1 )...
Efficient delivery of stabilized nucleic acids (NAs) into cells and release the NA payload are crucial points in transfection process. Here we report on fabrication a nanoscopic cellular carrier that is additionally combined with label-free intracellular sensor device, based biocompatible fluorescent nanodiamond particles. The sensing function engineered nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. device used for monitoring cells....
A monophosphonate analogue of H4dota, 1,4,7,10-tetraazacyclododecane-4,7,10-tris(carboxymethyl)-1-methylphosphonic acid (H5do3aP), and its complexes with lanthanides were synthesized. Multinuclear NMR studies reveal that, in aqueous solution, lanthanide(III) the ligand exhibit structures analogous to those H4dota complexes. Thus, central ion is nine-coordinate, surrounded by four nitrogen atoms, three acetate one phosphonate oxygen water molecule an apical position. For H5do3aP Ln(III) ions...
Polyhedral metallacarboranes are used mainly as ion-pairing agents and recently have been recognized potent inhibitors of HIV protease. They characterized by exceptional hydrophobicity, rigid geometry, delocalized negative charge, behavior, strong acidity their conjugated acids. The completely novel phenomenon, association these promising pharmaceutical tectons in aqueous solutions, is described here. behavior two structural types metallacarboranes, [bis(1,2-dicarbollide)cobaltate(1−)]...
HIV protease (PR) is a prime target for rational anti-HIV drug design. We have previously identified icosahedral metallacarboranes as novel class of nonpeptidic inhibitors. Now we show that substituted are potent and specific competitive inhibitors drug-resistant PRs prepared either by site-directed mutagenesis or cloned from HIV-positive patients. Molecular modeling explains the inhibition profile their unconventional binding mode.
HIV protease is a primary target for the design of virostatics. Screening libraries non-peptide low molecular weight compounds led to identification several new that inhibit PR in micromolar range. X-ray structure complex one them, dibenzo[b,e][1,4]diazepinone derivative, showed two molecules inhibitor bind active site. Covalent linkage such compound by two-carbon linker decrease inhibition constant resulting 3 orders magnitude. Molecular modeling shows these dimeric inhibitors form crucial...