A5031941235- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Diamond and Carbon-based Materials Research
- Force Microscopy Techniques and Applications
- Cellular transport and secretion
- Analytical Chemistry and Sensors
- Nicotinic Acetylcholine Receptors Study
- Electrochemical sensors and biosensors
- Carbon Nanotubes in Composites
- Electrochemical Analysis and Applications
- Neural dynamics and brain function
- Cardiac electrophysiology and arrhythmias
- Molecular Junctions and Nanostructures
- Nerve injury and regeneration
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Integrated Circuits and Semiconductor Failure Analysis
- Neurogenesis and neuroplasticity mechanisms
- Regulation of Appetite and Obesity
- Neuroscience of respiration and sleep
- Alzheimer's disease research and treatments
- Biochemical Analysis and Sensing Techniques
- Advanced Memory and Neural Computing
- Photoreceptor and optogenetics research
University of Turin
2015-2024
Istituto Nazionale di Ricerca Metrologica
2023
Torino e-district
2022-2023
Istituto Nazionale di Fisica Nucleare, Sezione di Torino
2023
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2023
Charles University
2023
International Training Centre of the ILO
2020
University of Trento
2017
National Interuniversity Consortium for the Physical Sciences of Matter
2005-2016
Neuroscience Institute
1997-2013
We studied wild-type (WT) and Cav1.3 −/− mouse chromaffin cells (MCCs) with the aim to determine isoform of L-type Ca 2+ channel (LTCC) BK channels that underlie pacemaker current controlling spontaneous firing. Most WT-MCCs (80%) were spontaneously active (1.5 Hz) highly sensitive nifedipine BayK-8644 (1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)phenyl]-3-pyridinecarboxylic acid, methyl ester). Nifedipine blocked firing, whereas increased threefold firing rate. The two...
Temperature is one of the most relevant parameters for regulation intracellular processes. Measuring localized subcellular temperature gradients fundamental a deeper understanding cell function, such as genesis action potentials, and metabolism. Here, we detect first time variations (1{\deg}C) associated with potentiation depletion neuronal firing, exploiting nanoscale thermometer based on optically detected magnetic resonance in nanodiamonds. Our results provide tool assessing spiking...
Abstract Understanding the human brain is one of most significant challenges 21st century. As theoretical studies continue to improve description complex mechanisms that regulate biological processes, in parallel numerous experiments are conducted enrich or verify these predictions also with aim extrapolating more accurate models. In fields magnetometry and thermometry, among various sensors proposed for application, nitrogen‐vacancy (NV) centers emerging as a promising solution due their...
α 1H T‐type channels recruited by β 1 ‐adrenergic stimulation in rat chromaffin cells (RCCs) are coupled to fast exocytosis with the same Ca 2+ dependence of high‐threshold channels. Here we show that RCCs exposed chronic hypoxia (CH) for 12–18 h 3% O 2 express comparable densities functional depolarize resting and contribute low‐voltage exocytosis. Following hypoxia, most exhibited already available at −50 mV gating, pharmacological molecular features as isoform. Chronic had no effects on...
Long-lasting exposures to brain-derived neurotrophic factor (BDNF) accelerate the functional maturation of GABAergic transmission in embryonic hippocampal neurons, but molecular bases this phenomenon are still debated. Evidence favor a postsynaptic site action has been accumulated, most data support presynaptic effect. A crucial issue is whether enhancement evoked IPSCs (eIPSCs) induced by BDNF attributable an increase any elementary parameters controlling neurosecretion, namely probability...
Mouse chromaffin cells (MCCs) of the adrenal medulla possess fast-inactivating Nav channels whose availability alters spontaneous action potential firing patterns and Ca(2+)-dependent secretion catecholamines. Here, we report MCCs expressing large densities neuronal Nav1.3 Nav1.7 that carry little or no subthreshold pacemaker currents can be slowly inactivated by 50% upon slight membrane depolarization. Reducing Nav1.3/Nav1.7 tetrodotoxin sustained depolarization near rest leads to a switch...
Excessive iron levels are believed to contribute the development of neurodegenerative disorders by promoting oxidative stress and harmful protein clustering. Novel chelation treatments that can effectively remove excess while minimizing negative effects on nervous system being explored. This study focuses creation evaluation innovative nanobubble (NB) formulations, shelled with various polymers such as glycol-chitosan (GC) conjugated deferoxamine (DFO), enhance their ability bind iron....
T-type channels are expressed weakly or not at all in adult rat chromaffin cells (RCCs) and there is contrasting evidence as to whether they play a functional role catecholamine secretion. Here we show that 3-5 days after application of pCPT-cAMP, most RCCs grown serum-free medium high density low-voltage-activated without altering the expression characteristics high-voltage-activated channels. The cAMP-recruited increased with time displayed typical biophysical pharmacological properties...
We studied the effect of Amyloid β 1-42 oligomers (Abeta42) on Ca2+ dependent excitability profile hippocampal neurons. Abeta42 is one beta peptides produced by proteolytic processing amyloid precursor protein and participates in initiating event triggering progressive dismantling synapses neuronal circuits. Our experiments cultured network reveal that increases intracellular concentration 46% inhibits firing discharge 19%. More precisely, differently regulates ryanodine (RyRs), NMDA...
Micro-Graphitic Single Crystal Diamond Multi Electrode Arrays (µG-SCD-MEAs) have been used so far as amperometric sensors for detecting catecholamines from chromaffin cells or adrenal gland slices. Besides having time resolution and sensitivity comparable with carbon fiber electrodes (CFEs), that represent the gold standard amperometry, μG-SCD-MEAs also advantages of simultaneous multisite detection, high biocompatibility implementation amperometric/potentiometric protocols, aimed at...
Chronic application of brain-derived neurotrophic factor (BDNF) induces new selective synthesis non-L-type Ca2+ channels (N, P/Q, R) at the soma cultured hippocampal neurons. As N- and P/Q-channels support neurotransmitter release in hippocampus, this suggests that BDNF-treatment may enhance synaptic transmission by increasing expression presynaptic as well. To address issue we studied long-term effects BDNF on miniature stimulus-evoked GABAergic rat embryo We found increased frequency...
Besides regulating energy balance and reducing body-weight, the adipokine leptin has been recently shown to be neuroprotective antiapoptotic by promoting neuronal survival after excitotoxic oxidative insults. Here, we investigated firing properties of mouse hippocampal neurons effects pretreatment on hypoxic damage (2 hours, 3% O2). Experiments were carried out means microelectrode array (MEA) technology, monitoring activity from 11 18 days in vitro (DIV). Under normoxic conditions,...
The quantal release of catecholamines from neuroendocrine cells is a key mechanism which has been investigated with broad range materials and devices, among carbon-based such as carbon fibers, diamond-like carbon, nanotubes nanocrystalline diamond. In the present work we demonstrate that MeV-ion-microbeam lithographic technique can be successfully employed for fabrication an all-carbon miniaturized cellular bio-sensor based on graphitic micro-channels embedded in single-crystal diamond...
A microstructured graphitic 4x4 multielectrode array was embedded in a single crystal diamond substrate (4x4 {uG-SCD MEA) for real-time monitoring of exocytotic events from cultured chromaffin cells and adrenal slices. The current approach relies on the development parallel ion beam lithographic technique, which assures time effective fabrication extended arrays with reproducible electrode dimensions. reported device is suitable performing amperometric voltammetric recordings high...