A5006075298- Cancer, Hypoxia, and Metabolism
- Nuclear Physics and Applications
- Mitochondrial Function and Pathology
- Diamond and Carbon-based Materials Research
- Metabolism, Diabetes, and Cancer
- Metabolism and Genetic Disorders
- Cancer-related Molecular Pathways
- Ion-surface interactions and analysis
- Radiation Detection and Scintillator Technologies
- Nuclear reactor physics and engineering
- Epigenetics and DNA Methylation
- Radiopharmaceutical Chemistry and Applications
- Particle Detector Development and Performance
- Nuclear physics research studies
- Nuclear Materials and Properties
- Particle accelerators and beam dynamics
- High-pressure geophysics and materials
- Pancreatic and Hepatic Oncology Research
- Cancer Mechanisms and Therapy
- Radiation Therapy and Dosimetry
- Boron Compounds in Chemistry
- Metalloenzymes and iron-sulfur proteins
- Radioactive contamination and transfer
- Radiation Effects in Electronics
- RNA modifications and cancer
Czech Academy of Sciences, Nuclear Physics Institute
2016-2025
Institute of Clinical and Experimental Medicine
2023-2024
Czech Academy of Sciences, Institute of Biotechnology
2018-2024
Czech Academy of Sciences
2002-2024
Charles University
2024
Research Centre Rez
2014-2021
Institute of Nuclear Physics
2021
Nuclear Research Institute Rez (Czechia)
2014-2019
University of Chemistry and Technology, Prague
2003-2018
Griffith University
2011-2016
Mitochondrial complex II (CII) has been recently identified as a novel target for anti-cancer drugs. Mitochondrially targeted vitamin E succinate (MitoVES) is modified so that it preferentially localized to mitochondria, greatly enhancing its pro-apoptotic and activity. Using genetically manipulated cells, MitoVES caused apoptosis generation of reactive oxygen species (ROS) in CII-proficient malignant cells but not their CII-dysfunctional counterparts. inhibited the dehydrogenase (SDH)...
Abstract High biocompatibility, variable size ranging from ≈5 nm, stable luminescence its color centers, and simple carbon chemistry for biomolecule grafting make nanodiamond (ND) particles an attractive alternative to molecular dyes drug‐delivery. A novel method is presented that can be used remote monitoring of chemical processes in biological environments based on changes photoluminescent (PL) nitrogen‐vacancy (NV) centers ND. The NV driven chemically by alternating the surface potential...
A novel approach for preparation of ultra-bright fluorescent nanodiamonds (fNDs) was developed and the thermal kinetic optimum NV center formation identified. Combined with a new oxidation method, this enabled particles that were roughly one order magnitude brighter than prepared commonly used procedures.
Expression of the HER2 oncogene in breast cancer is associated with resistance to treatment, and Her2 may regulate bioenergetics. Therefore, we investigated whether disruption electron transport chain (ETC) a viable strategy eliminate Her2high disease.We demonstrate that cells tumors have increased assembly respiratory supercomplexes (SCs) complex I-driven respiration vitro vivo. They are also highly sensitive MitoTam, novel mitochondrial-targeted derivative tamoxifen. Unlike tamoxifen,...
Deferoxamine (DFO) represents a widely used iron chelator for the treatment of overload. Here we describe use mitochondrially targeted deferoxamine (mitoDFO) as novel approach to preferentially target cancer cells. The agent showed marked cytostatic, cytotoxic, and migrastatic properties in vitro, it significantly suppressed tumor growth metastasis vivo. underlying molecular mechanisms included (i) impairment iron-sulfur [Fe-S] cluster/heme biogenesis, leading destabilization loss activity...
Cellular senescence is a form of cell cycle arrest that limits the proliferative potential cells, including tumour cells. However, inability immune cells to subsequently eliminate senescent from organism may lead tissue damage, inflammation, enhanced carcinogenesis and development age-related diseases. We found anticancer agent mitochondria-targeted tamoxifen (MitoTam), unlike conventional agents, kills cancer without inducing in vitro vivo. Surprisingly, it also selectively eliminates both...
Core-shell nanoparticles based on fluorescent nanodiamonds coated with a biocompatible N-(2-hydroxypropyl)methacrylamide copolymer shell were developed for background-free near-infrared imaging of cancer cells. The particles showed excellent colloidal stability in buffers and culture media. After conjugation cyclic RGD peptide they selectively targeted integrin αvβ3 receptors glioblastoma cells high internalization efficacy.
Nanodiamonds have been gaining popularity in a variety of applications. These studies compare the cellular response to fluorescent, detonation, functionalized and drug-loaded nanodiamonds.
Accumulating evidence suggests that breast cancer involves tumour-initiating cells (TICs), which play a role in initiation, metastasis, therapeutic resistance and relapse of the disease. Emerging drugs target TICs are becoming focus contemporary research. Mitocans, group compounds induce apoptosis by destabilising their mitochondria, showing potential killing TICs. In this project, we investigated mitochondrially targeted vitamin E succinate (MitoVES), recently developed mitocan, for its...
Summary Metformin, the first drug chosen to be tested in a clinical trial aimed target biology of aging per se, has been clinically exploited for decades absence complete understanding its therapeutic targets or chemical determinants. We here outline systematic chemoinformatics approach computationally predict biomolecular metformin. Using several structure‐ and ligand‐based software tools reference databases containing 1,300,000 compounds more than 9,000 binding sites protein cavities, we...
Abstract Pancreatic cancer is one of the hardest-to-treat types neoplastic diseases. Metformin, a widely prescribed drug against type 2 diabetes mellitus, being trialed as an agent pancreatic cancer, although its efficacy low. With idea delivering metformin to molecular target, mitochondrial complex I (CI), we tagged with vector, triphenylphosphonium group. Mitochondrially targeted (MitoMet) was found kill panel cells three four orders magnitude more efficiently than for parental compound....
Abstract Fluorescent nanodiamonds (FNDs) are vital to many emerging nanotechnological applications, from bioimaging and sensing quantum nanophotonics. Yet, understanding engineering the properties of fluorescent defects in remain challenging. The most comprehensive study date is presented, optical physical five different nanodiamond samples, which nitrogen‐vacancy (NV) centers created using fabrication techniques. FNDs' fluorescence spectra, lifetime, spin relaxation time ( T 1 )...
Efficient delivery of stabilized nucleic acids (NAs) into cells and release the NA payload are crucial points in transfection process. Here we report on fabrication a nanoscopic cellular carrier that is additionally combined with label-free intracellular sensor device, based biocompatible fluorescent nanodiamond particles. The sensing function engineered nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. device used for monitoring cells....
Abstract The miniaturized radiation camera MiniPIX TPX3 is designed for detailed and wide-range measurements of mixed-radiation fields present in many applications such as radiotherapy space outer orbit. highly integrated instrumentation utilizes a single connector control readout flexible quick deployment. device features an option to process the registered data on same with limited resolution basic particle-type resolving power. A novel processing technique exploits detector high...
Abstract Position and directional-sensitive spectrometry of energetic charged particles can be performed with high resolution wide dynamic range (energy, direction) the hybrid semiconductor pixel detectors Timepix/Timepix3. The choice sensor material, thickness, properties such as reverse bias voltage, greatly determine detector sensitivity resolving power for particle tracking. We investigated evaluated spectral tracking deposited energy linear-energy-transfer (LET) spectra Timepix3...
A plausible strategy to reduce tumor progress is the inhibition of angiogenesis. Therefore, agents that efficiently suppress angiogenesis can be used for suppression. We tested antiangiogenic potential a mitochondrially targeted analog α-tocopheryl succinate (MitoVES), compound with high propensity induce apoptosis.MitoVES was found kill proliferating endothelial cells (ECs) but not contact-arrested ECs or deficient in mitochondrial DNA, and suppressed vitro by inducing accumulation reactive...
The core issue for the implementation of diamond NV centre qubits technology is sensitive readout spin state. We have recently demonstrated photoelectric detection magnetic resonances (PDMR), anticipated to be faster and more than optical (ODMR). Here we report on a PDMR contrast 9 % - three times enhanced compared previous work shallow N-implanted diamond. Based ab-initio modelling, demonstrate novel one-photon ionization dual-beam protocol. predict that this scheme significantly less...
Aims: To assess the effect of mitochondrially targeted vitamin E (VE) analogs on mitochondrial function and biogenesis. Results: Mitochondrially succinate (MitoVES) is an efficient inducer apoptosis in cancer cells. Here, we show that unlike its untargeted counterpart α-tocopheryl succinate, MitoVES suppresses proliferation cells at sub-apoptotic doses by way affecting DNA (mtDNA) transcripts. We found strongly level displacement loop transcript followed those mtDNA genes coding for subunits...
We show that fluorescent nanodiamonds (FNDs) are among the few types of nanosensors enable direct optical reading noncovalent molecular events. The unique sensing mechanism is based on switching between negatively charged and neutral states NV centers which induced by interaction FND surface with molecules.
Fluorination of diamonds modulates their optical and electromagnetic properties creates surfaces with increased hydrophobicity. In addition, fluorination nanodiamonds has been recently shown to stabilize fluorescent nitrogen‐vacancy centers, which can serve as extremely sensitive single atomic defects in a vast range sensing applications from quantum physics high‐resolution biological imaging. Traditionally, carbon nanomaterials achieved using harsh complex experimental conditions, creating...