A5056267400- Wound Healing and Treatments
- Diabetic Foot Ulcer Assessment and Management
- Exercise and Physiological Responses
- MicroRNA in disease regulation
- Biochemical Acid Research Studies
- Muscle metabolism and nutrition
- Pressure Ulcer Prevention and Management
- Antioxidant Activity and Oxidative Stress
- Angiogenesis and VEGF in Cancer
- Vitamin C and Antioxidants Research
- Adipose Tissue and Metabolism
- Bacterial biofilms and quorum sensing
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Mesenchymal stem cell research
- Coenzyme Q10 studies and effects
- Planarian Biology and Electrostimulation
- Electrospun Nanofibers in Biomedical Applications
- Mitochondrial Function and Pathology
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Burn Injury Management and Outcomes
- Redox biology and oxidative stress
- Immune cells in cancer
Indiana University – Purdue University Indianapolis
2018-2025
University of Pittsburgh
2023-2025
McGowan Institute for Regenerative Medicine
2023-2025
Indiana University School of Medicine
2018-2024
Indiana University Health
2018-2024
Center for Neuroscience and Regenerative Medicine
2023-2024
Cook Biotech (United States)
2024
California Institute for Regenerative Medicine
2024
Ann Arbor Center for Independent Living
2024
The Ohio State University
2012-2023
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level by either degradation or translational repression of a target mRNA. Encoded in genome most eukaryotes, miRNAs have been proposed to specifically up 90% human genes through process known as miRNA-guided RNA silencing. For first time, we sought test how myocardial ischaemia-reperfusion (IR) changes miR expression.Following 2 and 7 h IR sham operation, tissue was collected subjected...
Chronic inflammation is a characteristic feature of diabetic cutaneous wounds. We sought to delineate novel mechanisms involved in the impairment resolution At wound-site, efficient dead cell clearance (efferocytosis) pre-requisite for timely and successful healing.
Angiogenesis plays a central role in wound healing. Among many known growth factors, vascular endothelial factor (VEGF) is believed to be the most prevalent, efficacious, and long-term signal that stimulate angiogenesis wounds. Whereas direct of copper facilitate has been evident two decades ago, specific targets action remained unclear. This report presents first evidence showing inducible VEGF expression sensitive angiogenic potential may harnessed accelerate dermal contraction closure. At...
Previously we have reported in vitro evidence suggesting that H2O2 may support wound healing by inducing VEGF expression human keratinocytes (C. K. Sen et al., 2002, J. Biol. Chem. 277, 33284–33290). Here, test the significance of regulating vivo. Using Hunt–Schilling cylinder approach present first site contains micromolar concentrations H2O2. At site, low supported process, especially p47phox- and MCP-1-deficient mice which endogenous generation is impaired. Higher doses adversely...
Abnormal cardiac ryanodine receptor (RyR2) function is recognized as an important factor in the pathogenesis of heart failure (HF). However, specific molecular causes underlying RyR2 defects HF remain poorly understood. In present study, we used a canine model chronic to test hypothesis that HF-related alterations are caused by posttranslational modification reactive oxygen species generated failing heart. Experimental approaches included imaging cytosolic ([Ca(2+)](c)) and sarcoplasmic...
At an injury site, efficient clearance of apoptotic cells by wound macrophages or efferocytosis is a prerequisite for the timely resolution inflammation. Emerging evidence indicates that microRNA-21 (miR-21) may regulate inflammatory response. In this work, we sought to elucidate significance miR-21 in regulation efferocytosis-mediated suppression innate immune response, key process implicated resolving inflammation following injury. An increased expression inducible was noted...
Ischemia complicates wound closure. Here, we are unique in presenting a murine ischemic model that is based on bipedicle flap approach. Using this of wounds have sought to elucidate how microRNAs may be implicated limiting re-epithelialization under hypoxia, major component ischemia. Ischemia, evaluated by laser Doppler as well hyperspectral imaging, limited blood flow and lowered tissue oxygen saturation. EPR oximetry demonstrated the had p O 2 <10 mm Hg. Ischemic suffered from...
Objective: The objective of this work was to causatively link biofilm properties bacterial infection specific pathogenic mechanisms in wound healing. Background: Staphylococcus aureus is one the four most prevalent species identified chronic wounds. Causatively linking pathology challenging. Thus, isogenic mutant stains S. with varying degree formation ability studied an established preclinical porcine model infection. Methods: Isogenic strains ( ΔrexB > USA300 ΔsarA ) biofilm-forming...
Inflammation, following injury, induces cellular plasticity as an inherent component of physiological tissue repair. The dominant fate wound macrophages is unclear and debated. Here we show that two-thirds all granulation fibroblasts, otherwise known to be mesenchymal origin, are derived from myeloid cells which likely macrophages. Conversion fibroblast-like impaired in diabetic wounds. In cross-talk between keratinocytes cells, miR-21 packaged extracellular vesicles (EV) required for cell...