A5080476804- Breast Implant and Reconstruction
- Wound Healing and Treatments
- Lymphatic System and Diseases
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Reconstructive Surgery and Microvascular Techniques
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Diagnosis and Treatment of Venous Diseases
- Antifungal resistance and susceptibility
- Pressure Ulcer Prevention and Management
- Genetic Neurodegenerative Diseases
- Reconstructive Facial Surgery Techniques
- Fungal Infections and Studies
- Diabetic Foot Ulcer Assessment and Management
- Immune cells in cancer
- RNA Research and Splicing
- Antimicrobial Peptides and Activities
- Breast Cancer Treatment Studies
- Bacterial biofilms and quorum sensing
- Phagocytosis and Immune Regulation
- Body Contouring and Surgery
- Organ and Tissue Transplantation Research
- Carbon dioxide utilization in catalysis
Indiana University School of Medicine
2019-2024
Indiana University – Purdue University Indianapolis
2019-2024
Indiana University
2020-2024
Creative Commons
2022-2023
Center for Neuroscience and Regenerative Medicine
2021-2023
Indiana University Health
2019-2023
Indianapolis Zoo
2021-2023
Cleveland Research (United States)
2023
Brown University
2022
Providence College
2022
Objective: The objective of this work was to causatively link biofilm properties bacterial infection specific pathogenic mechanisms in wound healing. Background: Staphylococcus aureus is one the four most prevalent species identified chronic wounds. Causatively linking pathology challenging. Thus, isogenic mutant stains S. with varying degree formation ability studied an established preclinical porcine model infection. Methods: Isogenic strains ( ΔrexB > USA300 ΔsarA ) biofilm-forming...
Inflammation, following injury, induces cellular plasticity as an inherent component of physiological tissue repair. The dominant fate wound macrophages is unclear and debated. Here we show that two-thirds all granulation fibroblasts, otherwise known to be mesenchymal origin, are derived from myeloid cells which likely macrophages. Conversion fibroblast-like impaired in diabetic wounds. In cross-talk between keratinocytes cells, miR-21 packaged extracellular vesicles (EV) required for cell...
In chronic wounds, biofilm infects host tissue for extended periods of time. This work establishes the first preclinical model wound infection aimed at addressing long-term response. Although biofilm-infected wounds did not show marked differences in closure, repaired skin demonstrated compromised barrier function. observation is clinically significant, because it leads to notion that even if a infected closed, as observed visually, may be complicated by presence failed skin, which likely...
Abstract Milk fat globule epidermal growth factor-factor 8 (MFG-E8) is a peripheral glycoprotein that acts as bridging molecule between the macrophage and apoptotic cells, thus executing pivotal role in scavenging of cells from affected tissue. We have previously reported cell clearance activity or efferocytosis compromised diabetic wound macrophages. In this work, we test hypothesis MFG-E8 helps resolve inflammation, supports angiogenesis, accelerates closure. MFG-E8−/− mice displayed...
Huntington's disease (HD) is caused by the expansion of N-terminal polymorphic poly Q stretch protein huntingtin (HTT). Deregulated microRNAs and loss function transcription factors recruited to mutant HTT aggregates could cause characteristic transcriptional deregulation associated with HD. We observed earlier that expressions miR-125b, miR-146a miR-150 are decreased in STHdhQ111/HdhQ111 cells, a model for HD comparison those wild type STHdhQ7/HdhQ7 cells. In present manuscript, we show...
Hyperglycemia (HG) induces genome-wide cytosine demethylation. Our previous work recognized miR-200b as a critical angiomiR, which must be transiently downregulated to initiate wound angiogenesis. Under HG, downregulation is not responsive injury. Here, we demonstrate that HG may drive vasculopathy by epigenetic modification of miR promoter. In human microvascular endothelial cells (HMECs), also lowered DNA methyltransferases (DNMT-1 and DNMT-3A) compromised function manifested diminished...
Epithelial to mesenchymal transition (EMT) and wound vascularization are two critical interrelated processes that enable cutaneous healing. Zinc finger E-box binding homeobox 1 (ZEB1), primarily studied in the context of tumor biology, is a potent EMT activator. ZEB1 also known contribute endothelial cell survival as well stimulate angiogenesis. The role wounds was assessed using Zeb1+/− mice, Zeb1−/− mice not viable. Quantitative stable isotope labeling by amino acids culture (SILAC)...
Expansion of polymorphic glutamine (Q) numbers present at the protein Huntingtin (Htt) beyond 36Q results in its misfolding and aggregation, aggregates recruit several other proteins. Here we show that HYPK, initially identified as an Htt-interacting partner by yeast two-hybrid assay, physically interacts with N-terminal Htt Neuro2A cells alters distribution formed 40Q. HYPK also kinetics mutated Htt-mediated aggregate formation. Fluorescence recovery after photobleaching studies reveal...
We observed earlier that expressions of several micro RNAs (miRNAs) are altered in a cell model Huntington's disease (HD). As genes involved different neurodegenerative diseases targeted by miRNAs, we searched databases to find out whether Huntingtin gene (HTT), mutation which causes HD, is target any miRNA. Among many miRNAs predicted HTT, showed using various experimental approaches miR-214, miR-150, miR-146a and miR-125b could both human HTT mouse Htt. Luciferase reporter vectors...
Non-invasive, repeated interrogation of the same wound is necessary to understand tissue repair continuum. In this work, we sought test significance non-invasive high-frequency high-resolution ultrasound technology for such interrogation. High-frequency imaging was employed investigate healing under fetal and adult conditions. Quantitative cellularity elastic strain obtained visualization unresolved inflammation using Vevo software. Hemodynamic properties blood flow in artery supplying...
Objective: This work addressing complexities in wound infection, seeks to test the reliance of bacterial pathogen Pseudomonas aeruginosa (PA) on host skin lipids form biofilm with pathological consequences. Background: PA causes chronicity. Both CDC as well NIH recognizes infection a threat leading Chronic wounds lower extremities often lead surgical limb amputation. Methods: An established preclinical porcine chronic model, infected or ceramidase mutant (PA ∆Cer ), was used. Results: We...
Infections are problematic in postmastectomy implant-based reconstruction with infection rates as high 30%. Strategies to reduce the risk of have demonstrated various efficacies. A prolonged course systemic, oral antibiotics has not shown evidence-based benefit. Although absorbable antibiotic beads been described for orthopedic procedures and pressure wounds, their use well studied during breast reconstruction, particularly prepectoral implant placement. The purpose this study was evaluate...
Deep inferior epigastric artery perforator (DIEP) flap is a common method of breast reconstruction. Enhanced recovery after surgery (ERAS) postoperative protocols have been used to optimize patient outcomes and facilitate shorter hospital stays. The effect expectations on length stay (LOS) DIEP has not evaluated. purpose this study was investigate whether affect LOS. A retrospective chart review performed for patients undergoing flaps reconstruction from 2017 2020. All were managed with the...