A5014636503- Zebrafish Biomedical Research Applications
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
- Acute Myeloid Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Botany and Plant Ecology Studies
- Education, Psychology, and Social Research
- Alkaline Phosphatase Research Studies
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Neurogenesis and neuroplasticity mechanisms
- ATP Synthase and ATPases Research
- Immune cells in cancer
- Hemoglobinopathies and Related Disorders
- Mesenchymal stem cell research
- Drug Transport and Resistance Mechanisms
- RNA Interference and Gene Delivery
- Medicinal plant effects and applications
- Botanical Studies and Applications
University of Hradec Králové
2023
Charles University
2023
Masaryk University
2015-2019
Reactive oxygen species (ROS) are important regulators of cellular functions. In embryonic stem cells, ROS suggested to influence differentiation status. Regulated formation is catalyzed primarily by NADPH‐dependent oxidases (NOXs). Apocynin and diphenyleneiodonium frequently used inhibitors NOXs; however, both exhibit uncharacterized effects not related NOXs inhibition. Interestingly, in our model mouse cells we demonstrate low expression NOXs. Therefore aimed clarify potential side these...
Hypoxia is involved in the regulation of stem cell fate, and hypoxia‐inducible factor 1 (HIF‐1) master regulator hypoxic response. Here, we focus on effect hypoxia intracellular signaling pathways responsible for mouse embryonic (ES) maintenance. We employed wild‐type HIF‐1 α ‐deficient ES cells to investigate response ERK, Akt, STAT3 pathways. Cultivation 1% O 2 24 h resulted strong dephosphorylation ERK its upstream kinases a lesser extent Akt an HIF‐1‐independent manner, while...
The activation of p38alpha kinase mediates cell response to various extracellular factors including many interleukins and growth important for haematopoiesis. role was previously analysed in particular haematopoietic cells. In this study the first time, haematopoiesis studied using a model continuous development pluripotent embryonic stem cells vitro. expression transcripts associated with potential formation specific colonies were compared between wild-type mutant gene-depleted absence led...