A5021892062- Ocular Surface and Contact Lens
- Polydiacetylene-based materials and applications
- Surfactants and Colloidal Systems
- Advancements in Transdermal Drug Delivery
- Advanced Drug Delivery Systems
- Advanced Glycation End Products research
- Carbohydrate Chemistry and Synthesis
- Urticaria and Related Conditions
- Proteoglycans and glycosaminoglycans research
- Fatty Acid Research and Health
- Food Chemistry and Fat Analysis
- Metabolism and Genetic Disorders
- Biochemical and Molecular Research
- Advanced Fiber Optic Sensors
- Cancer, Hypoxia, and Metabolism
Helsinki University Hospital
2020-2024
University of Helsinki
2020-2024
The tear film lipid layer (TFLL) covers the film, stabilizing it and providing a protective barrier against environment. TFLL is divided into polar non-polar sublayers, but interplay between classes in these sublayers structure-function relationship of remains poorly characterized. This study aims to provide insight function by elucidating interactions lipids at molecular level.Mixed films O-acyl-ω-hydroxy fatty acids (OAHFA) or phospholipids cholesteryl esters (CE) were used as model TFLL....
In healthy eyes, the tear film lipid layer (TFLL) is considered to act as an evaporation resistant barrier, which prevents eyes from drying. Seeking understand mechanisms behind resistance of TFLL, we studied mixtures wax esters and O-acyl-ω-hydroxy fatty acids. Analyzing their self-assembly biophysical properties led new discoveries concerning structure function TFLL. We discovered how these lipids self-assemble at air–water interface form efficient antievaporative demonstrating for first...
The tear film lipid layer (TFLL) is a unique biological membrane that serves pivotal role in the maintenance of ocular surface health. Reaching an overarching understanding functional principle TFLL has been hampered by lack insights into structural and roles played individual classes. To bridge this knowledge gap, we herein focus on studying films formed principal classes scattering methods. Through grazing incidence X-ray diffraction reflectivity studies, reveal quantitative data about...
The tear film lipid layer (TFLL) that covers the ocular surface contains several unique classes, including O-acyl-ω-hydroxy fatty acids, type I-St diesters, and II diesters. While TFLL represents a biological barrier plays central role in stabilizing entire film, little is known about properties roles of individual species. This because their isolation from samples sufficient quantities tedious task. To provide access to these species pure form, shed light on properties, we here report...
The tear film lipid layer (TFLL) is a unique biological membrane of importance to the maintenance ocular surface health. underlying factors at play, e.g. ability retard evaporation and offer protection from environment, are all closely connected properties individual components their interplay. TFLL contains ultra-long polar species such as O-acyl-ω-hydroxy fatty acids, type I-St diesters II diesters, which considered important for its proper function. Herein, we have synthesized model...
The tear film lipid layer (TFLL) plays a vital part in maintenance of ocular health and represents unique biological barrier comprising unusual specialized classes species. wax cholesteryl esters (WEs CEs) constitute roughly 80–90% the TFLL. majority species these are branched it is therefore surprising that synthesis properties second largest category species, i.e., anteiso-branched remain poorly characterized. In this study, we have developed total route completed detailed NMR...
The tear film lipid layer (TFLL) is important to the maintenance of ocular surface health. Surprisingly, information on individual roles myriad unique lipids found therein limited. most abundant species are wax esters (WE) and cholesteryl (CE), and, especially their branched analogs. isolation these from TFLL has proved be tedious, as a result, insights biophysical profiles role in currently lacking. Herein, we circumvent issues by total synthesis iso-methyl WEs CEs TFLL. Through detailed...
Purpose: The majority of dry eye disease (DED) patients suffer from the tear film lipid layer (TFLL) instability issues associated with increased evaporation water ocular surface. Therefore, we developed a liposomal formulation based on selected TFLL classes that would restore stability and retard aqueous fluid. Methods: was prepared by thin hydration method subsequently characterized intermittently standard techniques for at least 6 months. mitochondrial metabolic activity (MTT) assay human...
The
Dry eye disease (DED), the most common ocular disorder, reduces quality of life for hundreds millions people annually. In healthy eyes, tear film lipid layer (TFLL) stabilizes and moderates evaporation rate fluid. >80% DED cases, these central features are compromised leading to instability excessive Herein we assess potential liposomal formulations featuring phosphatidylcholines tailored species from wax ester O-acyl-ω-hydroxy fatty acid categories in targeting this defect. The developed...
The tear film lipid layer (TFLL) is a unique biological membrane of importance to the maintenance ocular surface health. underlying factors at play, e.g. ability retard evaporation and offer protection from environment, are all closely connected properties individual components their interplay. TFLL contains ultra-long polar species such as O-acyl-ω-hydroxy fatty acids, type I-St diesters II diesters, which considered important for its proper function. Herein, we have synthesized model...