A5032794113- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Nanopore and Nanochannel Transport Studies
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Ocular Surface and Contact Lens
- Pancreatic function and diabetes
- Advanced Drug Delivery Systems
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Grouting, Rheology, and Soil Mechanics
- Antimicrobial agents and applications
- Gut microbiota and health
- Fungal Biology and Applications
- Cellular transport and secretion
- Molecular Sensors and Ion Detection
- Neonatal Respiratory Health Research
- Biochemical Analysis and Sensing Techniques
- Yersinia bacterium, plague, ectoparasites research
- Surfactants and Colloidal Systems
- Vibrio bacteria research studies
- Pharmacological Effects of Natural Compounds
Charles University
2019-2025
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2019-2022
Abstract Finding effective antibiotics against multi-resistant strains of bacteria has been a challenging race. Linker-Evolved-Group-Optimized-Lipophosphonoxins (LEGO-LPPOs) are small modular synthetic antibacterial compounds targeting the cytoplasmic membrane. Here we focused on understanding reasons for variable efficacy selected LEGO-LPPOs ( LEGO-1 , LEGO-2 LEGO-3 and LEGO-4 ) differing in hydrophobic linker module structure length. LEGO-1–4 permeabilized membrane Staphylococcus aureus...
Abstract Colicin production in Escherichia coli ( E . ) strains represents an important trait with regard to microbial survival and competition the complex intestinal environment. A novel colicin type, Z (26.3 kDa), was described as a product of original producer, extraintestinal B1356 strain, isolated from anorectal abscess 17 years-old man. The 4,007 bp plasmid (pColZ) completely sequenced activity cza immunity czi genes were identified. are transcribed opposite directions encode for 237...
We developed modular antimicrobial compounds capable of inducing cytoplasmic membrane perforation. This structure–activity relationship study focuses on the role length their alkenyl hydrophobic module.
We introduce and study a multicomponent lipid film mimicking composition of the human lung surfactant. It consists phospholipids with various headgroups tail saturation. Furthermore, it includes cholesterol oxidized lipids. Langmuir trough fluorescence microscopy experiments are combined fully atomistic molecular dynamics simulations. The considered mixtures form complex interfacial films properties modulated by lateral compression. Cholesterol laterally condenses, lipids expand films; both...
Colicin U is a protein produced by the bacterium Shigella boydii (serovars 1 and 8). It exerts antibacterial activity against strains of enterobacterial genera Escherichia Here, we report that colicin forms voltage-dependent pores in planar lipid membranes; its single-pore conductance was found to be about 22 pS M KCl at pH 6 under 80 mV asolectin bilayers. In agreement with high degree homology between their C-terminal domains, shares some pore characteristics related colicins A B. are...
Fonticins are phage tail-like bacteriocins produced by the Gram-negative bacterium Pragia fontium from family Budviciaceae. This produces contractile-type particles that adsorb on surface of sensitive bacteria and penetrate cell wall, probably during contraction, in a way similar to type VI secretion system. We characterized pore-forming activity fonticins using both living cells vitro model membranes. Using potassium leakage assay, we show able permeabilize cells. On black lipid membranes,...
Abstract Linker-Evolved-Group-Optimized-Lipophosphonoxins (LEGO-LPPO) are small synthetic modular peptidomimetics with promising antimicrobial activity. The LEGO-LPPO mechanism of antibacterial action has been determined to be the depolarization and disruption bacterial membranes. Their nature is advantageous for fine tuning their biological properties. In order optimize structure even further, it important understand interaction membranes at molecular level. this work, we present synthesis...
<title>Abstract</title> Finding effective antibiotics against multi-resistant strains of bacteria has been a challenging race. LEGO-lipophosphonoxins (LEGO-LPPOs) are small modular synthetic antibacterial compounds targeting the cytoplasmic membrane. Here we focused on understanding reasons for variable efficacy selected LEGO-LPPOs (LEGO-1, LEGO-2, LEGO-3, and LEGO-4) differing in hydrophobic linker module structure length. LEGO-1-4 permeabilized membrane <italic>Staphylococcus...