A5038330912- Lipid Membrane Structure and Behavior
- Molecular Sensors and Ion Detection
- Orbital Angular Momentum in Optics
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Biosensors and Analytical Detection
- Analytical Chemistry and Chromatography
- Electrohydrodynamics and Fluid Dynamics
- Magnetism in coordination complexes
- Surfactants and Colloidal Systems
- Mass Spectrometry Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Advanced Chemical Sensor Technologies
- RNA Interference and Gene Delivery
- Analytical Methods in Pharmaceuticals
- Nanoplatforms for cancer theranostics
- Lanthanide and Transition Metal Complexes
- Advanced Proteomics Techniques and Applications
- Photoreceptor and optogenetics research
- Advanced biosensing and bioanalysis techniques
- Biomedical Research and Pathophysiology
- Lysosomal Storage Disorders Research
- Antibiotics Pharmacokinetics and Efficacy
- Laser Applications in Dentistry and Medicine
- Electrochemical Analysis and Applications
Imperial College London
2007-2019
Bristol Robotics Laboratory
2019
University of Bristol
2017
Liverpool John Moores University
2015-2016
Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge their physical state and structure. However, mapping viscosity diffusion in complex composition is currently a non-trivial technical challenge. We report fluorescence lifetime spectroscopy imaging (FLIM) meso-substituted BODIPY molecular rotor localised leaflet model various compositions. prepare large giant unilamellar vesicles (LUVs GUVs) containing phosphatidylcholine (PC) lipids...
Small-molecule amphiphilic species such as many drug molecules frequently exhibit low-to-negligible aqueous solubility, and generally have no identified transport proteins assisting their distribution, yet are able to rapidly penetrate significant distances into patient tissue even cross the blood-brain barrier. Previous work has a mechanism of translocation driven by acid-catalysed lipid hydrolysis biological membranes, process which is catalysed presence cationic molecules. In this study,...
A major trend in analytical chemistry is the miniaturization of laboratory instrumentation. We present a portable high-performance liquid chromatography system for field based chemical analyses.
This paper demonstrates spatially selective sampling of the plasma membrane by implementation time-multiplexed holographic optical tweezers for Smart Droplet Microtools (SDMs). High speed (>1000fps) dynamical hologram generation was computed on graphics processing unit a standard display card and controlled user friendly LabView interface. Time multiplexed binary holograms were displayed in real time mirrored to ferroelectric Spatial Light Modulator. SDMs manufactured with both liquid cores...
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We present a rapid and robust technique for the sampling of membrane-associated proteins from surface single, live cell their subsequent deposition onto solid-supported lipid bilayer. As proof principle, this method has been used to extract green fluorescent protein (EGFP) labelled K-ras located at inner leaflet plasma membrane colon carcinoma cells transfer them an S-layer supported bilayer system. The is non-destructive, meaning that both are intact after operation, offering potential...
Cationic amphiphilic drugs (CADs), such as haloperidol, are able to translocate through biological membrane bilayers via a chemically activated degradation of the phospholipid fabric membrane, which may constitute third type drug translocation process. We present evidence that this effect is dependent on formulation. The choice counterion shown influence rate bilayer hydrolysis, leading either promotion reaction or drug's precipitation condensation
We demonstrate controllable poration within ≈1 µm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates.This technique will allow new experiments in single-cell biology, particularly neuroscience.As our understanding the fundamental mechanistic processes underpinning biology expands, so does need for high-precision tools to dissection heterogeneity and stochastic that dominate at single-and sub-cellular level.Here, we highly...
Combining tightly focused laser beams with surface-treated microtools enables injection and surface biopsies on individual live cells, minimal disruption to their normal function.
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