Abstract Rapid antibiotic susceptibility tests (ASTs) are an increasingly important part of clinical care as antimicrobial resistance (AMR) becomes more common in bacterial infections. Here, we use the spatial distribution fluorescently labelled ribosomes to detect intracellular changes associated with E. coli cells using a convolutional neural network (CNN). By ribosome-targeting probes, one fluorescence image provides data for cell segmentation and phenotyping. Using 60,382 from...

We demonstrate the rapid capture, enrichment, and identification of bacterial pathogens using Adaptive Channel Bacterial Capture (ACBC) devices. Using controlled tuning device backpressure in polydimethylsiloxane (PDMS) devices, we enable formation capture regions capable trapping bacteria from low cell density samples with near 100% efficiency. The technical demands to prepare such devices are much lower compared conventional methods for can be achieved simple benchtop fabrication methods....

Abstract Polycyclic aromatic hydrocarbons (PAHs) are considered priority hazardous substances due to their carcinogenic activity and risk public health. Strict regulations in place limiting release into the environment, but enforcement is hampered by a lack of adequate field-testing procedure, instead relying on sending samples centralised analytical facilities. Reliably monitoring levels PAHs field challenge, owing field-deployable methods able separate, identify, quantify complex mixtures...

A major trend in analytical chemistry is the miniaturization of laboratory instrumentation. We present a portable high-performance liquid chromatography system for field based chemical analyses.

Abstract Antibiotic resistance is an urgent global health challenge, necessitating rapid diagnostic tools to combat its threat. This study uses citizen science and image feature analysis profile the cellular features associated with antibiotic in Escherichia coli . Between February April 2023, we conducted Infection Inspection project, which 5273 volunteers made 1,045,199 classifications of single-cell images from five E. strains, labelling them as antibiotic-sensitive or...

Abstract Rapid antibiotic susceptibility tests (ASTs) are an increasingly important part of clinical care as antimicrobial resistance (AMR) becomes more common in bacterial infections. Here, we use the spatial distribution fluorescently labelled ribosomes to detect intracellular changes associated with single E. coli cells using a convolutional neural network (CNN). By ribosome-targeting probes, fluorescence cell image provides data for segmentation and phenotyping. Using 50,722 images from...

Abstract We present a simple, facile method to micropattern planar metal electrodes defined by the geometry of microfluidic channel network template. By introducing aqueous solutions into reversibly adhered PDMS devices desiccation instead flow, we are able produce difficult pattern “dead end” or discontinuous features with ease. characterize fabricated using this and perform electrical lysis mammalian cancer cells demonstrate their use as part an antibody capture assay for GFP. Cell in...

Surgery for hand trauma accounts a significant proportion of the plastic surgery training curriculum. The aim this study was to create standardized simulation module fracture fixation with Kirschner wire (K-wire) techniques residents framework that universally hones their skill and prepares them first encounter in clinical setting.A step-ladder approach 6 levels difficulty on 3-dimensional (3D) printed ex vivo biomimetics employed cohort 20 (n = 20). Assessment skills using score system...

Abstract We demonstrate the rapid capture, enrichment, and identification of bacterial pathogens using Adaptive Channel Bacterial Capture (ACBC) devices. Using controlled tuning device backpressure in polydimethylsiloxane (PDMS) devices, we enable formation capture regions capable trapping bacteria from low cell density samples with near 100% efficiency. The technical demands to prepare such devices are much lower compared conventional methods for can be achieved simple benchtop fabrication...

Microsurgical training involves practice in ex vivo models during the early learning curve, and poor instrument handling by inexperienced microsurgeons can cause damage to microsurgical instrumentation or clamps, which is particularly costly. To address this, we demonstrate development, design, manufacturing, application of 3 different types 3-dimensional printed microvascular clamps an simulation model. This report provides evidence a low-cost easily accessible device that facilitates...

Summary Mallet finger deformity is a common and debilitating injury of the fingertip, accounting for 10% all tendon ligament injuries. It involves disruption terminal extensor mechanism distal phalanx. Patients can experience significant pain swelling fingertip have morbidity without treatment. Nonoperative treatment using joint immobilization with splints mainstay management. Traditionally, prefabricated thermoplastic been utilized; however, issues comfort skin complications such as...

Great strides toward routine single-cell analyses have been made over the last decade, particularly in field of transcriptomics. For proteomics, amplification is not currently possible and has necessitated development ultrasensitive platforms capable performing such on single cells. These are improving terms throughput multiplexability but still fall short relation to more established methods as fluorescence microscopy. However, microscopy rely intensity a proxy for protein abundance...

Summary: The deep inferior epigastric perforator (DIEP) flap has become the free of choice for autologous breast reconstruction. However, anastomoses DIEP pedicles to internal mammary vessels in chest wall are difficult due restricted access and depth vessels. Successful performance such demanding procedures necessitates advanced requirements microsurgical training models. current chicken thigh model been used acquire skills, allowing early learning curve trainees practice repeatedly...

One of the main drivers within field bottom-up synthetic biology is to develop artificial chemical machines, perhaps even living systems, that have programmable functionality. Numerous toolkits exist generate giant unilamellar vesicle-based cells. However, methods able quantitatively measure their molecular constituents upon formation an underdeveloped area. We report cell quality control (AC/QC) protocol using a microfluidic-based single-molecule approach, enabling absolute quantification...

Summary Patient preparation for hand surgery often necessitates skin via the use of an assistant to hold arm be operated on in mid-air while disinfectant is applied. This study introduces a three-dimensional printed stand that decreases dead time during preparation, also enabling more efficient assistant. The devices were customized anatomy patients and then successfully used having general or regional anesthesia. A practical, reusable, effective has been developed applied both adult...

The expansion of the global economy in last century has been a wide enabler for rapid economic growth and technological development. It can however be said that this interwoven fabric rendered most modern-day societies closely interdependent to one another, rendering them able function normally only under very delicate set conditions. ongoing COVID-19 pandemic shown how extraordinary surges patient morbidity mortality result healthcare crises which manifest severe economic, social political...

Performing microvascular anastomoses can be challenging when oedematous fluids and blood continuously flood/compromise the field of view. Intermittent irrigation suctioning disturb workflow, requires an assistant, increase risk arterial thrombosis, from vessels being drawn into suction drains. We developed patented a novel, 3D-printed background device with microfluidic capabilities, to provide autonomous, continuous optimise operator autonomy efficiency. then tested this in rat femoral...

Often cellular behavior and responses are analyzed at the population level where of many cells pooled together as an average result masking rich single cell within a complex population. Single protein detection quantification technologies have made remarkable impact in recent years. Here we describe practical flexible analysis platform based on addressable droplet microarrays. This study describes how absolute copy numbers target proteins may be measured with resolution. The tumor suppressor...

Abstract Antibiotic resistance is an urgent global health challenge, necessitating rapid diagnostic tools to combat its escalating threat. This study introduces innovative approaches for expedited bacterial antimicrobial profiling, addressing the critical need swift clinical responses. Between February and April 2023, we conducted Infection Inspection project, a citizen science initiative in which public could participate advancing susceptibility testing method based on single-cell images of...

Counting Proteins In Single Cells With Addressable Droplet Microarrays - Video. JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch our articles. is the world-leading producer provider of science videos with mission improve research, journals, education. Millions scientists, educators students at thousands universities, colleges, hospitals biopharmaceutical companies worldwide use for their teaching learning.

The microsurgical anastomosis is integral to the success of autologous-free tissue transfer. Successful performance this procedure relies strongly on operator dexterity, which can be made more challenging when blood and edematous fluids obscure field view. Workflow impeded by intermittent irrigation suctioning, necessitating presence an assistant, with risk arterial thrombosis, from vessels being drawn into suction drains. To negate these current disadvantages minimize barrier entry...

Summary We demonstrate the design, manufacture, and deployment of first custom-made 3-dimensional (3D)-printed hand retractor for pollicization procedure. Radiological images patient’s were taken preoperatively to measure anatomical dimensions guide design device in a patient-precise manner. The 3D-printed, sterilizable, was autoclaved successfully used on patient that underwent procedure our unit. radiolucency fluency enabled by ability exchange between different positions demonstrated...