A5014096950- Lipid Membrane Structure and Behavior
- Molecular Sensors and Ion Detection
- Cell death mechanisms and regulation
- Surfactants and Colloidal Systems
- Advanced Fluorescence Microscopy Techniques
- Protein Structure and Dynamics
- Cancer-related Molecular Pathways
- Spectroscopy and Chemometric Analyses
- Spectroscopy Techniques in Biomedical and Chemical Research
- Education, Psychology, and Social Research
- Regulation of Appetite and Obesity
- Synthesis and bioactivity of alkaloids
- Retinoids in leukemia and cellular processes
- DNA and Nucleic Acid Chemistry
- Mitochondrial Function and Pathology
- Analytical Chemistry and Chromatography
- Lymphatic System and Diseases
- RNA Interference and Gene Delivery
- Biochemical Analysis and Sensing Techniques
- Power Quality and Harmonics
- Sphingolipid Metabolism and Signaling
- Cardiovascular Disease and Adiposity
- High voltage insulation and dielectric phenomena
- Protein Interaction Studies and Fluorescence Analysis
- Spectroscopy and Quantum Chemical Studies
Charles University
2008-2021
Czech Academy of Sciences
2010-2013
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2008-2012
Czech Academy of Sciences, Institute of Molecular Genetics
2010-2012
Institute of Endocrinology
2008
Physical properties of oxidized phospholipid (OxPL) membranes consisting binary mixtures 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 10 mol % one two OxPLs, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) or 1-palmitoyl-2-(5′-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), were investigated experimentally computationally. Fluorescence solvent relaxation (SR) fluorescence correlation spectroscopy z-scan (FCS z-scan) show increased headgroup hydration mobility,...
Although there were experimental indications that phospholipid bilayers hydrated with D2O express different biophysical properties compared hydration by ordinary H2O, a molecular concept for this behavior difference was only recently proposed dynamics simulations study [T. Róg et al., J. Phys. Chem. B, 2009, 113, 2378–2387]. Here we attempt to verify those theoretical predictions fluorescence measurements on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and...
Abstract Later stages of secondary lymphedema are associated with the massive deposition adipose tissue (AT). The factors driving lymphedema-associated AT (LAT) expansion in humans remain rather elusive. We hypothesized that LAT could be based on alterations metabolic, adipogenic, immune and/or angiogenic qualities AT. samples were acquired from upper limbs 11 women unilateral breast cancer-related and healthy without lymphedema. Additional control group female cancer survivors was used to...
Membranes undergo severe changes under oxidative stress conditions due to the creation of oxidized phospholipid (OxPL) species, which possess molecular properties quite different from their parental lipid components. These OxPLs play crucial roles in various pathological disorders and occurrence is involved onset intrinsic apoptosis, a fundamental pathway programmed mammalian cell death. However, mechanisms by these lipids can exert apoptotic action via host membranes (e.g., altering...
Ghrelin is a gut peptide produced mainly by stomach, well known to induce appetite stimulatory actions. Obestatin, recently identified derived from preproghrelin, was initially described antagonize effect of ghrelin on food intake. The postprandial response obestatin and its relationship with in humans remains unknown. We therefore investigated the total ghrelin, acyl desacyl neuropeptide Y (NPY) high-carbohydrate breakfast (1 604 kJ) eight healthy women (age: 24.2+/-0.82 years; BMI...
Within the past decade single molecule techniques contributed significantly to understanding of mechanism DNA condensation induced by various kinds condensers. While observation molecules bigger than 100 kbp (kilobasepairs) can be achieved fluorescence microscopy, smaller visualized directly only via methods allowing higher resolution like atomic force microscopy (AFM), with drawback that observed particles interfere surface. Here, we introduce a robust utilization novel technique based on...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a death with selective antitumor activity. However, many primary tumors are TRAIL resistant. Previous studies reported that roscovitine, cyclin-dependent kinase inhibitor, sensitized various solid cancer cells to TRAIL. We show roscovitine and demonstrate synergistic cytotoxicity in hematologic malignant cell lines cells. Pretreatment of TRAIL-resistant leukemia induced enhanced cleavage death-inducing signaling complex-bound...
Two fluorescence spectroscopy concepts, correlation and time correlated single photon counting (TCSPC) are employed in lifetime (FLCS) - a relatively new technique with several experimental benefits. In FLCS experiments, pulsed excitation is used data stored special time-tagged time-resolved mode. Mathematical treatment of TCSPC decay patterns distinct fluorophores their mixture enables to calculate autocorrelation functions each the thus diffusion properties concentrations can be determined...