A5034535415- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- S100 Proteins and Annexins
- Quantum Dots Synthesis And Properties
- Nanoparticle-Based Drug Delivery
- Advanced Nanomaterials in Catalysis
- Ion Channels and Receptors
- Calcium signaling and nucleotide metabolism
- Antimicrobial Peptides and Activities
- Mass Spectrometry Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Force Microscopy Techniques and Applications
- Luminescence and Fluorescent Materials
- Advanced Battery Materials and Technologies
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- Neuropeptides and Animal Physiology
- Protein Kinase Regulation and GTPase Signaling
- Sepsis Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Connexins and lens biology
- Cardiac, Anesthesia and Surgical Outcomes
University of Münster
2018-2023
Institute of Molecular Biology
2021-2023
Blackberry (United States)
2018
Universidade Federal de Pernambuco
2013-2017
Universidade Federal de Mato Grosso
2010
Mechanisms keeping leukocytes distant of local inflammatory processes in a resting state despite systemic release triggers are pivotal requirement for avoidance overwhelming inflammation but ill defined. Dimers the alarmin S100A8/S100A9 activate Toll-like receptor-4 (TLR4) extracellular calcium concentrations induce S100A8/S100A9-tetramers preventing TLR4-binding and limiting their activity. So far, only antimicrobial functions released (calprotectin) described. It is demonstrated that...
Rationale: Capillary leakage frequently occurs during sepsis and after major surgery is associated with microvascular dysfunction adverse outcome. Procalcitonin a well-established biomarker in inflammation without known impact on vascular integrity. Objectives: We determined how procalcitonin induces endothelial hyperpermeability targeting protects barrier Methods: In prospective observational clinical study, levels were assessed 50 patients who underwent cardiac correlated to postoperative...
The use of Quantum Dots (QDs) as fluorescent probes for understanding biological functions has emerged an advantageous alternative over application conventional dyes. Intracellular delivery QDs is currently a specific field research. When are tracking target in live cells, they mostly applied extracellular membrane labeling. In order to study intracellular molecules and structures it necessary deliver free into the cell cytosol. this work, we adapted freeze thaw method encapsulate water...
Cyclodextrin vesicles decorated with luminescent Ir(<sc>iii</sc>)-complexes are used as contrast agents for cell imaging.
The protein-mediated formation of membrane contacts is a crucial event in many cellular processes ranging from the establishment organelle to docking vesicles target membrane. Annexins are Ca2+ regulated membrane-binding proteins implicated providing such contacts; however, molecular basis bridging by annexins not fully understood. We addressed this central question using annexin A2 (AnxA2) that functions secretory vesicle exocytosis possibly bridges. By quantitatively analyzing contact...
Abstract Cholesterol is an essential component of cellular membranes regulating the structural integrity and fluidity biological bilayers processes such as signal transduction membrane trafficking. However, tools to investigate role dynamics cholesterol in live cells are still scarce often show limited applicability. To address this, we previously developed a class imidazolium-based analogs, CHIMs. Here confirm that CHIM integration characteristics largely mimic those cholesterol....
The transport of membrane impermeable compounds into cells is a prerequisite for the efficient cellular delivery hydrophilic and amphiphilic drugs. Transport cell's cytosolic compartment should ideally be controllable it involve biologically compatible degradable vehicles. Addressing these challenges, nanocontainers based on cyclodextrin amphiphiles that are stabilized by biodegradable peptide shell developed their potential to deliver fluorescently labeled cargo human analyzed. Host-guest...
Upon proinflammatory challenges, endothelial cell surface presentation of the leukocyte receptor P-selectin, together with stabilizing co-factor CD63, is needed for capture and mediated via demand-driven exocytosis from Weibel-Palade bodies that fuse plasma membrane. We report neutrophil recruitment to activated endothelium significantly reduced in mice deficient endolysosomal cation channel TPC2 human primary cells pharmacological block. observe less CD63 signal whole-mount stainings...
The specific transport of amphiphilic compounds such as fluorescently labeled phospholipids into cells is a prerequisite for the analysis highly dynamic cellular processes involving these molecules, e.g., intracellular distribution and metabolism phospholipids. However, delivery remains challenge it should not affect physiological integrity morphology cell membrane. To address this, polymer nanocontainers based on redox-responsive cyclodextrin (CD) amphiphiles are prepared, their potential...
Abstract A dicationic imidazolium salt is described and investigated towards its application for gene transfer. The polar head group the long alkyl chains in backbone contribute to a lipid‐like behavior, while an ammonium provides ability crucial electrostatic interaction transfection process. Detailed biophysical studies regarding impact on biological membrane models propensity of vesicle fusion are presented. Fluorescence spectroscopy, atomic force microscopy confocal fluorescence show...
Annexin A2 (AnxA2) is a cytosolic Ca2+ regulated membrane binding protein that can induce lipid domain formation and plays role in exocytosis endocytosis. To better understand the mode of annexin-membrane interaction, we analyzed membrane-bound AnxA2 assemblies by employing novel 3-armed chemical crosslinker specific mutant proteins. Our data show forms crosslinkable oligomers upon to membranes containing negatively charged phospholipids. mutants with amino acid substitutions residues...
Lipid mimetics are pivotal tools for lipid biology research. Herein, a bifunctional cholesterol mimetic was designed and investigated its potential to enable concurrent exploration of cellular distributions protein interactions.
The dissipative quartz crystal microbalance technique is a simple and label-free approach to measure simultaneously the mass uptake viscoelastic properties of absorbed/immobilized on sensor surfaces, allowing measurements interaction proteins with solid-supported such as lipid bilayers, in real-time high sensitivity. Annexins are highly conserved group phospholipid-binding that interact reversibly negatively charged headgroups via coordination calcium ions. Here, we describe protocol was...
The dissipative quartz crystal microbalance technique is a simple and label-free approach to measure simultaneously the mass uptake viscoelastic properties of absorbed/immobilized on sensor surfaces, allowing measurements interaction proteins with solid-supported such as lipid bilayers, in real-time high sensitivity. Annexins are highly conserved group phospholipid-binding that interact reversibly negatively charged headgroups via coordination calcium ions. Here, we describe protocol was...
Liposomes have been used to deliver DNA, drugs and, more recently, nanoparticles such as quantum dots, into living cells. Their electrostatic interaction with cell's surface (negatively charged) can lead membrane destabilization and/or fusion, facilitating intracellular release of those compounds. Nevertheless, cationic lipids modify cells homeostasis, depending on their concentration. In this study, we observed that the DOTAP lipid concentrations influence red blood (RBCs) homeostasis. We...
Dual-Responsive Nanocontainers In article number 2100694, Bart Jan Ravoo, Volker Gerke, and co-workers design nanocontainers based on cyclodextrin amphiphiles that are stabilized by a peptide shell redox-responsive crosslinker. These dual-responsive represent an efficient, biodegradable, tunable system for overcoming the plasma membrane as natural barrier membrane-impermeable cargo. The was conceived Sergej Kudruk composed Nina Knubel at University of Münster.
Abstract In response to pro-inflammatory challenges including pathogenic attack and tissue damage, the endothelial cell surface is rearranged present leukocyte-engaging receptors. The initial contact needed for leukocyte tethering rolling mediated via adhesion demand-driven exocytosis of Weibel-Palade bodies (WPB) that contain receptor P-selectin together with stabilizing co-factor CD63. We found diminished expression endolysosomal non-selective cation channel TPC2 or inhibition...