A5042730426- Hormonal Regulation and Hypertension
- Adrenal and Paraganglionic Tumors
- Cell Image Analysis Techniques
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Telomeres, Telomerase, and Senescence
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Eicosanoids and Hypertension Pharmacology
- Developmental Biology and Gene Regulation
- Hedgehog Signaling Pathway Studies
- Pituitary Gland Disorders and Treatments
- Neuropeptides and Animal Physiology
- Genomic variations and chromosomal abnormalities
- Neurological Disorders and Treatments
- Dental Implant Techniques and Outcomes
- Cardiovascular Effects of Exercise
- Genetics, Aging, and Longevity in Model Organisms
- Parathyroid Disorders and Treatments
- Microbial Metabolism and Applications
- Congenital heart defects research
- Thermoregulation and physiological responses
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Chromosomal and Genetic Variations
- Atherosclerosis and Cardiovascular Diseases
Fraunhofer Institute for Toxicology and Experimental Medicine
2023-2024
University of Münster
2018-2022
Pain and Rehabilitation Medicine
2020-2022
Max Planck Institute for Molecular Biomedicine
2020-2021
University Hospital Münster
2019-2021
Medical University of Warsaw
2000-2015
University of Bern
2014
Philipps University of Marburg
1996
Three-dimensional (3D) culture systems have fueled hopes to bring about the next generation of more physiologically relevant high-throughput screens (HTS). However, current protocols yield either complex but highly heterogeneous aggregates (‘organoids’) or 3D structures with less physiological relevance (‘spheroids’). Here, we present a scalable, HTS-compatible workflow for automated generation, maintenance, and optical analysis human midbrain organoids in standard 96-well-plates. The...
Patients with diabetes develop endothelial dysfunction shortly after onset that progresses to vascular disease underlying the majority of diabetes-associated comorbidities. Increased lipid peroxidation, mitochondrial calcium overload, and are characteristics dysfunctional cells in diabetic patients. We here identified targeting peroxidation product 12(S)-hydroxyeicosatetraenoic acid-induced [12(S)-HETE-induced] activation intracellularly located cation channel transient receptor potential...
This study was aimed at summarizing our experience in the management of 1 444 patients with incidentally found adrenal tumors observed a single endocrinological centre. Hormonal determinations were performed all beginning observation period to detect subclinical hyperfunction. The imaging phenotype on CT and MRI analyzed for defining malignant potential tumors. Based results these examinations we diagnosed among cohort probably benign masses 87%, 10% (adrenal carcinoma - 9%), metastases 3%....
Summary The recent development of a goat SNP genotyping microarray enables genome‐wide association studies in this important livestock species. We investigated the genetic basis black and brown coat colour Valais Blacknecked Coppernecked goats. A analysis using 50 BeadChip genotypes 22 cases 23 controls allowed us to map locus for chromosome 8. TYRP 1 gene is located within associated chromosomal region, variants cause similar phenotypes different thus considered as strong positional...
Rationale: Capillary leakage frequently occurs during sepsis and after major surgery is associated with microvascular dysfunction adverse outcome. Procalcitonin a well-established biomarker in inflammation without known impact on vascular integrity. Objectives: We determined how procalcitonin induces endothelial hyperpermeability targeting protects barrier Methods: In prospective observational clinical study, levels were assessed 50 patients who underwent cardiac correlated to postoperative...
Cell fate determination during development often requires morphogen transport from producing to distant responding cells. Hedgehog (Hh) morphogens present a challenge this concept, as all Hhs are synthesized terminally lipidated molecules that form insoluble clusters at the surface of While several proposed Hh modes tie directly into these unusual properties, crucial step relay cells receptors on remote remains unresolved. Using wing in Drosophila melanogaster model, we show and direct...
Abstract Telomeres as the protective ends of linear chromosomes, are synthesized by enzyme telomerase (TERT). Critically short telomeres essentially contribute to aging-related diseases and associated with a broad spectrum disorders known telomeropathies. In cardiomyocytes, telomere length is strongly correlated cardiomyopathies but it remains ambiguous whether cause or result disease. this study, we employed an inducible CRISPRi human induced pluripotent stem cell (hiPSC) line silence TERT...
Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues ("organoids"), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, sectioning is method for immunohistochemical analysis. However, it removes cells from their native niche and can result loss 3D context during analyses. Automated workflows require parallel processing analysis hundreds to thousands samples, mechanically...
Summary Meiotic breakpoint panels for human chromosomes 2, 3, 4, 5, 6, 7, 8, 9, 10, 13, 14, 15, 17, 18, 20 and X were constructed from genotypes the CEPH reference families. Each recombinant chromosome included has a well‐supported with to defined quantitative criteria. The at both low‐ resolution, useful first‐pass localization, high‐resolution, more precise placement. availability of such will reduce number genotyping experiments necessary order new polymorphisms respect existing genetic...
Objective: The aim of the ongoing study is to analyze retinal arteriolar structure in patients with pheochromocytoma (Pheo), a form secondary hypertension characterized by excessive catecholamine secretion as compared well-matched essential (EHT).Design and method: We examined 24 (15f/9m) Pheo (mean age 46.3 ± 11.8 years) age, gender, body mass index, glycemic status blood pressure levels matched (46.7 12.0 (EHT). In all evaluation plasma free normetanephrine (NMN) metanephrine (MN)...
We report on a 38 year old woman of Portuguese origin with peripheral ascending polyneuropathy for several years. Her family history was positive hereditary systemic amyloidosis. A familial amyloidotic type I variant (Val→Met30) the transthyretin molecule (prealbumin) diagnosed. In following months, symptoms worsened, gait became stepping, and urinary incontinence increased. order to prevent disease …
Telomeres as the protective ends of linear chromosomes, are synthesized by enzyme telomerase (TERT). Critically short telomeres essentially contribute to aging related diseases and associated with a broad spectrum disorders known telomeropathies. In cardiomyocytes, telomere length is strongly correlated cardiomyopathies but it remains ambiguous whether cause or result disease. this study, we employed an inducible CRISPRi human induced pluripotent stem cell (hiPSC) line silence TERT...
Objective: To present clinical characteristics of pheochromocytoma-paraganglioma syndromes based on Polish Pheochromocytoma Registry. Design and methods: The study included 59 participants with 33 index cases from the There were 29 patients (pts) SDHB (15 M, 14F, mean age 35 ± 18 yr), 23 pts SDHD (13 10F, 34 12 yr) 7 SDHC mutations (4 3F, 44 16 yr). Results: For median diagnosis was 36 (95%CI 33–51) yr compared 28 25–39) for 41 21–99) (NS). difference in age-related penetrance disease...