A5085079187- Cardiac Ischemia and Reperfusion
- Cardiac Arrest and Resuscitation
- Anesthesia and Neurotoxicity Research
- Sepsis Diagnosis and Treatment
- Mechanical Circulatory Support Devices
- Cardiac, Anesthesia and Surgical Outcomes
- Spine and Intervertebral Disc Pathology
- Immune Response and Inflammation
- Medical Practices and Rehabilitation
- Pelvic and Acetabular Injuries
- Trauma and Emergency Care Studies
- Spinal Fractures and Fixation Techniques
- Cardiac and Coronary Surgery Techniques
- Medical and Health Sciences Research
- Cell Adhesion Molecules Research
- Anesthesia and Sedative Agents
- Analytical Chemistry and Sensors
- Thermal Regulation in Medicine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Traumatic Brain Injury and Neurovascular Disturbances
- Intensive Care Unit Cognitive Disorders
- PARP inhibition in cancer therapy
- Gout, Hyperuricemia, Uric Acid
- Restraint-Related Deaths
- EEG and Brain-Computer Interfaces
University of Rostock
2006-2019
Klinikum Südstadt Rostock
2016-2019
Novem (Netherlands)
2017
University Hospital Münster
2016
Queen Mary University of London
2014
William Harvey Research Institute
2014
Remote ischemic preconditioning (RIPC) is reported to reduce biomarkers of and reperfusion injury in patients undergoing cardiac surgery, but uncertainty about clinical outcomes remains.We conducted a prospective, double-blind, multicenter, randomized, controlled trial involving adults who were scheduled for elective surgery requiring cardiopulmonary bypass under total anesthesia with intravenous propofol. The compared upper-limb RIPC sham intervention. primary end point was composite death,...
Abstract Background Ischemic myocardial damage accompanying coronary artery bypass graft surgery remains a clinical challenge. We investigated whether xenon anesthesia could limit in patients, as has been reported for animal ischemia models. Methods In 17 university hospitals France, Germany, Italy, and The Netherlands, low-risk elective, on-pump patients were randomized to receive xenon, sevoflurane, or propofol-based total intravenous maintenance. primary outcome was the cardiac troponin I...
Background Remote ischemic preconditioning ( RIPC ) has been suggested to protect against certain forms of organ injury after cardiac surgery. Previously, we reported the main results RIPHeart (Remote Ischemic Preconditioning for Heart Surgery) Study, a multicenter trial randomizing 1403 surgery patients receiving either or sham‐ . Methods and Results In this follow‐up paper, present 1‐year composite primary end point its individual components (all‐cause mortality, myocardial infarction,...
Objective: The fibrin-derived peptide Bβ15–42 has been shown to reduce infarct size in rodent models of ischemia-reperfusion injury. To increase its potential for translation into the clinic, we studied effects pigs, whose coronary anatomy is similar that humans. In addition, evaluated pharmacokinetics and safety several species, including Design: Animal study phase I trial. Setting: University hospital contract research laboratories. Subjects: Pigs/healthy volunteers. Interventions: Male...
The aim of our study was the identification genetic variants associated with postoperative complications after cardiac surgery. We conducted a prospective, double-blind, multicenter, randomized trial (RIPHeart). performed genome-wide association (GWAS) in 1170 patients both genders (871 males, 299 females) from RIPHeart-Study cohort. Patients undergoing non-emergent surgery were included. Primary endpoint comprises binary composite complication rate covering atrial fibrillation, delirium,...
The fibrin-derived peptide Bbeta15-42 (also called FX06) has been shown to reduce myocardial infarct size following ischemia/reperfusion. Hemorrhagic shock (HS) followed by volume resuscitation represents a similar scenario, whereby whole organism is vulnerable reperfusion injury.We subjected male farm-bred landrace pigs ( approximately 30 kg) HS withdrawing blood mean arterial pressure of 40 mm Hg for 60 minutes. Pigs were then resuscitated with shed and crystalloids minutes, at this time,...
Toll-like receptor 2 (TLR2) inhibition by function blocking antibodies (ABs) is associated with enhanced preservation of endothelial cell during vascular disease. In the present study, we investigated capacity TLR2-blocking ABs to modulate angiogenic response cells in vitro and vivo.Incubation mono- or polyclonal anti-TLR2 resulted increased tube formation, sprouting, migration compared controls. a mouse model hindlimb ischemia, using TLR2-deficient AB-treated wild-type mice new capillary...
Procalcitonin is used as a diagnostic tool for the identification and risk stratification of septic patients. plasma concentrations tightly correlate with severity ongoing inflammatory reaction can rise up to 10,000-fold. Impairment endothelial cell function plays an important role in pathogenesis hypotension disturbed organ perfusion during sepsis. We investigated possible effects procalcitonin itself on viability.Human cells were exposed 0.01 100 ng/mL permeability using transwells,...
Pharmacological protection from myocardial reperfusion injury, despite plenty of approaches, has still not been realized in humans. We studied the putative infarct size (IS)-sparing capacity poly(ADP-ribose)polymerase inhibitor, INO-1001, and focused on cardiac functional recovery during reperfusion. Male farm-bred Landrace pigs were subjected to 1-h left anterior descending coronary artery occlusion followed by 3 h (control). Infarct was determined triphenyltetrazolium chloride/Evans blue...
<b>Background:</b> Oxygen radicals have been implicated as important mediators in the early pathogenesis of acute pancreatitis, but mechanism by which they produce pancreatic tissue injury remains unclear. We have, therefore, investigated effects oxygen on isolated rat acinar cells to ultrastructure, cytosolic Ca<sup>2+</sup> concentration and energy metabolism. <b>Methods:</b> Acinar were exposed an radical-generating system consisting xanthine oxidase,...
Objectives: The fibrin-derived peptide Bβ 15–42 (FX06) has been proven to attenuate ischemia/reperfusion injury. We tested the hypothesis that improves survival rate and neurocognitive recovery after cardiopulmonary resuscitation. Design: Pig mouse model of Setting: Two university hospitals. Subjects: Pigs mice. Interventions: ( n = 16) were subjected 8-minute cardiac arrest. Successful resuscitated pigs 12) randomized either 3 mg/kg followed by a continuous infusion 1 mg/kg/hr for 5 hours...
Cardiac arrest (CA) followed by cardiopulmonary resuscitation (CPR) is associated with poor survival rate and neurofunctional outcome. Toll-like receptor 2 (TLR2) plays an important role in conditions of sterile inflammation such as reperfusion injury. Recent data demonstrated beneficial effects the administration TLR2-blocking antibodies ischemia/reperfusion In this study we investigated TLR2 for outcome after CA/CPR mice.Female TLR2-deficient (TLR2(-/-)) wild type (WT) mice were subjected...
Toll-like receptors (TLRs) play a crucial role in early host defense against microorganisms. receptor 2 (TLR2) polymorphisms have prevalence of 10%; functional defects TLR2 are associated with higher susceptibility toward gram-positive bacteria, and deficiency has been an impaired adrenal stress response. In the present study, we compared endogenous corticosterone production wild-type (WT) TLR2-deficient mice analyzed survival after hydrocortisone therapy during sepsis induced by cecal...
Introduction . Current treatment strategies for osteoporotic vertebral compression fractures (VCFs) focus on cement-associated solutions. Complications associated with cement application are leakage, embolism, adjacent fractures, and compromise in bony healing. This study comprises a validated VCF model sheep order to (1) evaluate new cementless fracture fixation technique using titanium mesh implants (TMIs) (2) demonstrate the healing capabilities VCFs. Methods Twelve 5-year-old Merino...
Cardiac arrest (CA) followed by cardiopulmonary resuscitation (CPR) is associated with high mortality and poor neurological outcome. We compared the effects of pravastatin simvastatin on survival neurofunction in a murine model CA/CPR. Pravastatin, hydrophilic statin, increased during 28-day follow-up period. This therapy was improved pulmonary function, reduced edema, endothelial cell function vitro. In contrast, lipophilic did not modulate but edema impaired function. Although may display...
This study analyses the effect of eptotermin α application into fractured vertebrae. It is hypothesized that capable to enhance bony healing osteoporotic spine. In 10 Merino sheep osteoporosis induction was performed by ovariectomy, corticosteroid therapy and calcium/phosphorus/vitamin D-deficient diet; followed standardized creation lumbar vertebral compression fractures (VCFs) type A3.1 consecutive fracture reduction/fixation using expandable mesh cages. Randomly, intravertebral (G1) or no...
A side-by-side comparison of the decarboxylation efficacy two pump-driven venovenous extracorporeal lung assist devices, i.e ., a first prototype new miniaturized ambulatory membrane ventilator, I-lung versus commercial system iLA-activve for more than period 72 hours in large animal model. Fifteen German Landrace pigs were anesthetized and underwent mechanical hypoventilation to induce severe hypercapnia. Decarboxylation was accomplished by either or via double lumen catheter jugular vein....