A5078817940- Hedgehog Signaling Pathway Studies
- Iron Metabolism and Disorders
- Cystic Fibrosis Research Advances
- Developmental Biology and Gene Regulation
- Hemoglobinopathies and Related Disorders
- Trace Elements in Health
- Genomics and Chromatin Dynamics
- Advanced Proteomics Techniques and Applications
- Wnt/β-catenin signaling in development and cancer
- Hippo pathway signaling and YAP/TAZ
- Potassium and Related Disorders
- Protease and Inhibitor Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Erythrocyte Function and Pathophysiology
- Pneumonia and Respiratory Infections
- Congenital Ear and Nasal Anomalies
- Mass Spectrometry Techniques and Applications
- Signaling Pathways in Disease
- Epigenetics and DNA Methylation
- Mycobacterium research and diagnosis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Genetic and rare skin diseases.
- Advanced Biosensing Techniques and Applications
- Peptidase Inhibition and Analysis
- Biomedical Ethics and Regulation
German Cancer Research Center
2022-2024
Heidelberg University
2022-2024
Helmholtz Association of German Research Centres
2023
Technical University of Denmark
2019-2022
University of Münster
2018-2021
Abstract Hemojuvelin (HJV) is a glycosylphosphatidylinositol-anchored protein of the repulsive guidance molecule family acting as bone morphogenetic (BMP) coreceptor to induce hepatic iron regulatory hepcidin. Hepcidin causes ubiquitination and degradation sole known exporter ferroportin, thereby limiting availability. The detailed signaling mechanism HJV in vivo has yet be investigated. In current manuscript, we used an established model adeno-associated virus (AAV)-mediated liver-specific...
Cell fate determination during development often requires morphogen transport from producing to distant responding cells. Hedgehog (Hh) morphogens present a challenge this concept, as all Hhs are synthesized terminally lipidated molecules that form insoluble clusters at the surface of While several proposed Hh modes tie directly into these unusual properties, crucial step relay cells receptors on remote remains unresolved. Using wing in Drosophila melanogaster model, we show and direct...
ABSTRACT Metazoan Hedgehog (Hh) morphogens are essential regulators of growth and patterning at significant distances from their source, despite being produced as N-terminally palmitoylated C-terminally cholesteroylated proteins, which firmly tethers them to the outer plasma membrane leaflet producing cells limits spread. One mechanism overcome this limitation is proteolytic processing both lipidated terminal peptides, called shedding, but molecular target site requirements for effective Hh...
Erythropoietin (Epo) ensures survival and proliferation of colony-forming unit erythroid (CFU-E) progenitor cells their differentiation to hemoglobin-containing mature erythrocytes. A lack Epo-induced responses causes embryonic lethality, but mechanisms regulating the dynamic communication cellular alterations organismal level remain unresolved. By time-resolved transcriptomics proteomics, we show that Epo induces in CFU-E a gradual transition from signature proteins indicative for...
Hedgehog (Hh) morphogens are involved in embryonic development and stem cell biology and, if misregulated, can contribute to cancer. One important post-translational modification with profound impact on Hh biofunction is its C-terminal cholesteroylation during biosynthesis. The current hypothesis that the cholesterol moiety a decisive factor association outer plasma membrane leaflet of producing cells, cell-surface multimerization, transport signaling. Yet, it not decided whether directly...
Abstract Iron storage disease is associated with cardiovascular manifestations, including various forms of cardiac arrhythmias unknown origin. In this study, iron overload were investigated in human iPSC-derived cardiomyocytes (hiPSC-CM) and hiPSC-derived sinus node-like pacemaker cells. Among other effects, leads to an increase the plasma membrane-anchored protease TMPRSS6. TMPRSS6 cleaves auxiliary subunit KCNE1 N-terminally thus modulates function both I Ks (KCNQ1/KCNE1 current) f...
Pseudomonas aeruginosa (PA) is the key bacterial pathogen in adult cystic fibrosis (CF) lung disease. We investigated how PA changes response of primary bronchial epithelial cells (pBECs) to human rhinovirus (HRV) infections. Differentiated pBECs were infected with for 16 days and then HRV. performed a quantitative proteomic analysis quantified Cytokines viral RNA by cytometric bead array (CBA) qPCR. In total 6500 proteins detected. HRV infection led increased abundance 50 CF 44 healthy...
Pseudomonas aeruginosa (PA) is the dominating bacterial pathogen in cystic fibrosis (CF) lungs, whereas respiratory viruses can trigger exacerbations of CF lung disease. We studied vitro how PA and Human Rhinovirus (HRV) infection influence each other. Differentiated bronchial epithelial cells from patients healthy controls (HC) were repeatedly infected with a clinical isolate for 20 days then coinfected HRV. Whole proteome analysis was combined N-terminal enrichment by tandem mass tag...