A5055280434- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- Pediatric Hepatobiliary Diseases and Treatments
- Pancreatitis Pathology and Treatment
- Diagnosis and treatment of tuberculosis
- Drug Transport and Resistance Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- RNA modifications and cancer
- Neonatal Health and Biochemistry
- Iron Metabolism and Disorders
- Microfluidic and Capillary Electrophoresis Applications
- Biliary and Gastrointestinal Fistulas
- Superconductivity in MgB2 and Alloys
- Phagocytosis and Immune Regulation
- Erythrocyte Function and Pathophysiology
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Antibiotic Resistance in Bacteria
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Cancer-related gene regulation
- Microfluidic and Bio-sensing Technologies
- Hydrogen Storage and Materials
- Gastrointestinal disorders and treatments
Instituto Politécnico Nacional
2013-2024
Center for Research and Advanced Studies of the National Polytechnic Institute
2015-2024
National Institute of Genomic Medicine
2015
Universidad Autónoma de la Ciudad de México
2005-2013
Centro de Investigación en Materiales Avanzados
1990-2006
Hospital General de México
1998
University of California, Berkeley
1990
Universidad de Alcalá
1990
University of Virginia
1988
Universidad del Centro de México
1981
In this paper, we attempted to define the role of phagocytosis in virulence Entamoeba histolytica. We have isolated, from a highly phagocytic and virulent strain, clone deficient phagocytosis. Trophozoites wild-type strain HM1:IMSS were fed with Escherichia coli CR34-Thy- grown on 5-bromo,2'-deoxyuridine. The trophozoites that had incorporated base analog through bacteria killed by irradiation 310 nm light. survivors, presumably defective phagocytosis, until log phase submitted two more...
Here, we present evidence that a cysteine protease (EhCP112) and protein with an adherence domain (EhADH112) form the Entamoeba histolytica 112 kDa adhesin. Immunoelectron microscopy immunofluorescence assays using monoclonal antibodies (mAbAdh) revealed that, during phagocytosis, adhesin is translocated from plasma membrane to phagocytic vacuoles. mAbAdh inhibited 54% adherence, 41% 35% 62% destruction of MDCK cell monolayers by live trophozoites their extracts respectively. We cloned 3587...
The cellular bases of the powerful cytolytic activity human protozoan parasite Entamoeba histolytica were explored by studying effect virulent strain HM1:IMSS on epithelial monolayers MDCK cells using a combination time-lapse microcinematography and transmission scanning electron microscopy. Early alterations cell membranes detected measuring changes in transepithelial electrical resistance mounted Ussing chambers. aggressive mechanism E. trophozoites was found to be complex, multifactorial...
Journal Article Isolation and Characterization of Phagocytosis- Virulence-Deficient Mutants Entamoeba histolytica Get access Mario Alberto Rodríguez, Rodríguez From the Department Genetics Molecular Biology, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, México, D.F. México Search for other works by this author on: Oxford Academic PubMed Google Scholar Esther Orozco Please address requests reprints to Dr. Orozco, Apartado Postal 14-740, 07000 D. F. The...
Entamoeba histolytica, the protozoan responsible for human amoebiasis, causes between 30,000 and 100,000 deaths per year worldwide. Amoebiasis is characterized by intestinal epithelial damage provoking severe diarrhea. However, molecular mechanisms which this are poorly understood. Here, we studied initial interactions E. histolytica EhCPADH112 virulence complex MDCK Caco-2 cells. By confocal microscopy, discovered that after contact with trophozoites or trophozoite extracts (TE), proteins...
The endosomal sorting complex required for transport (ESCRT) orchestrates cell membrane-remodeling mechanisms in eukaryotes, including endocytosis. However, ESCRT functions phagocytosis(ingestion of ≥ 250 nm particles), has been poorly studied. In macrophages and amoebae, phagocytosis is nutrition attack to other microorganisms cells.In Entamoeba histolytica, the voracious protozoan responsible human amoebiasis, phagocytosisis a land mark virulence. Here, we have investigated role ESCRT-III...
Journal Article Binding and Internalization of Rat Colonic Mucins by the Galactose/N-Acetyl-n-Galactosamine Adherence Lectin Entamoeba histolytica Get access Khrisendath Chadee, Chadee Search for other works this author on: Oxford Academic PubMed Google Scholar Michael L. Johnson, Johnson Esther Orozco, Orozco William A. Petri, Jr., Jr. Jonathan I. Ravdin Please address requests reprints to Dr. Ravdin, Box 419, School Medicine, University Virginia, Charlottesville, Virginia 22908. The...
During intestinal invasion, Entamoeba histolytica opens tight junctions (TJs) reflected by transepithelial electrical resistance (TEER) dropping. To explore the molecular mechanisms underlying this, we studied in vitro and vivo damage produced recombinant E. cysteine protease (rEhCP112) on TJ functions proteins. rEhCP112 reduced TEER Caco-2 cells a dose- time-dependent manner; EhCP112-overexpressing trophozoites provoked major epithelial injury compared to control trophozoites. penetrated...
EhCP112 is an Entamoeba histolytica protease that together with the EhADH112 protein forms EhCPADH complex involved in trophozoite virulence. Here, we produced recombinant and studied its relationships extracellular matrix components target cells. A DNA fragment containing pro-peptide mature enzyme was expressed bacteria as active (rEhCP112), whereas full gene signal peptide, a non-active protein. The only not expressed. rEhCP112 purified by affinity columns digested azocasein had strong...
Here, we investigated the role of EhVps32 protein (a member endosomal-sorting complex required for transport) in endocytosis Entamoeba histolytica, a professional phagocyte. Confocal microscopy, TEM and cell fractionation revealed cytoplasmic vesicles also located adjacent to plasma membrane. Between 5 30 min phagocytosis, was detected on some erythrocytes-containing phagosomes acidic nature, at 60 it returned appeared images membranous structures vicinity ingested erythrocytes. EhVps32,...
Entamoeba histolytica, the highly phagocytic protozoan causative of human amoebiasis lacks machinery to synthesize cholesterol. Here, we investigated presence NPC1 and NPC2 proteins in this parasite, which are involved cholesterol trafficking mammals. Bioinformatics analysis revealed one Ehnpc1 two Ehnpc2 genes. EhNPC1 appeared as a transmembrane protein both EhNPC2 peripheral membrane proteins. Molecular docking predicted that bind interact with each other. Genes were identified...
Entamoeba histolytica causes amoebiasis, an infection that kills 100,000 individuals each year. Metronidazole and its derivatives are currently used against this protozoan, but these drugs present adverse effects on human health. Here, we investigated the effect of resveratrol (a natural compound) E. trophozoites viability, as well influence parasite virulence. Trophozoites growth was arrested by 72 μM IC50 determined 220 at 48 h. Cells appeared smaller, rounded in clusters, with...
We present here the cloning and characterization of EhPgp1 multidrug resistance gene promoter isolated from Entamoeba histolytica drug-resistant mutant clone C2. The lacks typical TATA box transcriptional initiation sequences described for other E. promoters. major transcription site was located at ATG start codon. core within first 244 base pairs showed a higher chloramphenicol acetyltransferase expression in transfected trophozoites C2 than those sensitive A. Gel shift assays revealed...
This paper reports the identification of Ehp53, a p53-like Entamoeba histolytica protein, which binds to human p53 DNA consensus sequence (oli-p53). Monoclonal antibodies against (Ab-1 and Ab-2) recognized single 53 kDa spot in two-dimensional gels inhibited formation complexes produced by E. nuclear extracts oli-p53. Additionally, gene promoter sequences with high homology oli-p53 formed proteins that were abolished Ehp53 protein levels increased UV-irradiated trophozoites. was also...