A5087837931- Amoebic Infections and Treatments
- Iron Metabolism and Disorders
- Parasitic Infections and Diagnostics
- Diagnosis and treatment of tuberculosis
- Pediatric Hepatobiliary Diseases and Treatments
- Pancreatitis Pathology and Treatment
- Tuberculosis Research and Epidemiology
- Heme Oxygenase-1 and Carbon Monoxide
Universidad Autónoma de la Ciudad de México
2024
Universidad Autónoma de Guerrero
2018
Instituto Politécnico Nacional
2017
Center for Research and Advanced Studies of the National Polytechnic Institute
2017
During intestinal invasion, Entamoeba histolytica opens tight junctions (TJs) reflected by transepithelial electrical resistance (TEER) dropping. To explore the molecular mechanisms underlying this, we studied in vitro and vivo damage produced recombinant E. cysteine protease (rEhCP112) on TJ functions proteins. rEhCP112 reduced TEER Caco-2 cells a dose- time-dependent manner; EhCP112-overexpressing trophozoites provoked major epithelial injury compared to control trophozoites. penetrated...
Entamoeba histolytica trophozoites adhere to epithelium at the cell–cell contact and perturb tight junctions disturbing transepithelial electrical resistance. Behind are adherens (AJs) that reinforce them desmosomes (DSMs) maintain integrity. The damage produced AJs DMSs by this parasite is unknown. Here, we studied effect of trophozoites, EhCPADH complex, EhCP112 recombinant enzyme (rEhCP112) on AJ DSM proteins. We found degraded β-cat, E-cad, Dsp l/ll, Dsg-2 with participation EhCP112....
In Entamoeba histolytica, the EhADH adhesin together with EhCP112 cysteine protease, form a 124 kDa complex named EhCPADH. This participates in trophozoite adherence, phagocytosis and cytolysis of target cells. EhCPADH are both involved on epithelium damage, by opening tight junctions (TJ) reaching other intercellular junctions. is scaffold protein belonging to ALIX family that contains Bro1 domain, expresses at plasma membrane, endosomes cytoplasm trophozoites, also secreted medium....
Entamoeba histolytica, the causative agent of amebiasis, is third leading cause death among parasitic diseases globally. Its life cycle includes encystation, which has been mostly studied in invadens, responsible reptilian amebiasis. However, molecular mechanisms underlying this process are not fully understood. Therefore, we focused on identification and characterization Myb proteins, that regulate expression encystation-related genes various protozoan parasites. Through bioinformatic...
Entamoeba histolytica, the causative agent of amebiasis, is third leading cause death among parasitic diseases globally. Its life cycle includes encystation, which has been mostly studied in invadens, responsible for reptilian amebiasis. However, molecular mechanisms underlying this process are not fully understood. Therefore, we focused on identification and characterization Myb proteins, regulate expression encystation-related genes various protozoan parasites. Through bioinformatic...
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is a protozoan parasite that belongs to the Amoebozoa supergroup whose study related nucleocytoplasmic transport of proteins through nucleus poorly studied. In this work, we have performed
Entamoeba histolytica is the causative agent of human amoebiasis. Infection initiated by interaction pathogen with intestinal epithelial cells. This leads to disruption intercellular structures such as tight junctions (TJ). We recently showed that E. complex EhCPADH112, formed EhADH112 adhesin and EhCP112 cysteine protease, participates during invasion. To elucidate particular role in this process, we overexpressed adhesion molecule MDCK Expression was proven RT‐PCR western blot assays....
Entamoeba histolytica, the protozoan responsible for amoebiasis, is third leading cause of death among parasitic diseases. Encystation studied in invadens, reptilian amoebiasis. In E. gene expression differentially modulated during trophozoite differentiation, such as those encoding MYB-DBD-containing proteins. The proteins are transcription factors that regulate encystation-related genes different protozoa parasites. this work, we searched invadens MYB-domain-containing and identified 47...