A5011964221- Malaria Research and Control
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Computational Drug Discovery Methods
- HIV/AIDS drug development and treatment
- Parasites and Host Interactions
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
- Drug Transport and Resistance Mechanisms
- HIV Research and Treatment
- Biochemical and Molecular Research
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Evolution and Genetic Dynamics
- Drug-Induced Hepatotoxicity and Protection
- Insect Resistance and Genetics
- Parasite Biology and Host Interactions
- Insect symbiosis and bacterial influences
- Vector-borne infectious diseases
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Parasitic Diseases Research and Treatment
- Tuberculosis Research and Epidemiology
- Complement system in diseases
Broad Institute
2016-2025
Harvard University
2016-2025
Institute of Health Research, Epidemiological Surveillance and Training
2023
Boston University
1984-2022
Cheikh Anta Diop University
2005-2022
Hôpital Aristide Le Dantec
2022
Harvard University Press
1983-2020
ORCID
2020
Ministère de la Santé Publique
2020
Massachusetts Institute of Technology
1977-2017
The malaria parasite Plasmodium falciparum contains at least two genes related to the mammalian multiple drug resistance genes, and one of P. is expressed a higher level present in copy number strain that resistant drugs than sensitive drugs.
The RTS,S/AS01 vaccine targets the circumsporozoite protein of Plasmodium falciparum and has partial protective efficacy against clinical severe malaria disease in infants children. We investigated whether was specific to certain parasite genotypes at locus.We used polymerase chain reaction-based next-generation sequencing DNA extracted from samples 4985 participants survey polymorphisms. evaluated effect that polymorphic positions haplotypic regions within had on first episodes 1 year after...
Transmission represents a population bottleneck in the Plasmodium life cycle and key intervention target of ongoing efforts to eradicate malaria. Sexual differentiation is essential for this process, as only sexual parasites, called gametocytes, are infective mosquito vector. Gametocyte production rates vary depending on environmental conditions, but external stimuli remain obscure. Here, we show that host-derived lipid lysophosphatidylcholine (LysoPC) controls P. falciparum cell fate by...
Chemogenetic characterization through in vitro evolution combined with whole-genome analysis can identify antimalarial drug targets and drug-resistance genes. We performed a genome of 262
A detailed understanding of the human infectious reservoir is essential for improving malaria transmission-reducing interventions. Here we report a multi-regional assessment population-wide transmission potential based on 1209 mosquito feeding assays in endemic areas Burkina Faso and Kenya. Across both sites, identified 39 individuals. In high endemicity settings, individuals were identifiable by research-grade microscopy (92.6%; 25/27), whilst one three lowest setting was detected molecular...
Significance Traditional methods for estimating malaria transmission based on mosquito sampling are not standardized and unavailable in many countries sub-Saharan Africa. Such studies especially difficult to implement when is low, low the goal of elimination. Malaria-control efforts Senegal have resulted changes population genomics evidenced by increased allele sharing among parasite genomes, often including genomic identity between independently sampled parasites. Fitting an epidemiological...
ABSTRACT Plasmodium vivax causes heavy burdens of disease across malarious regions worldwide. Mature P. asexual and transmissive gametocyte stages occur in the blood circulation, it is often assumed that accumulation/sequestration tissues not an important phase their development. Here, we present a systematic study stage distributions infected nonhuman primate (NHP) malaria models as well from human infections. In comparative analysis transcriptomes falciparum blood-stage parasites, found...
Abstract Background Single nucleotide polymorphism (SNP) genotyping provides the means to develop a practical, rapid, inexpensive assay that will uniquely identify any Plasmodium falciparum parasite using small amount of DNA. Such an could be used distinguish recrudescence from re-infection in drug trials, monitor frequency and distribution specific parasites patient population undergoing treatment or vaccine challenge, for tracking samples determining purity isolates laboratory during...
Genetic variability of Plasmodium falciparum underlies its transmission success and thwarts efforts to control disease caused by this parasite. variation in antigenic, drug resistance, pathogenesis determinants is abundant, consistent with an ancient origin P. , whereas DNA at silent (synonymous) sites coding sequences appears virtually absent, a recent the To resolve paradox, we analyzed introns demonstrated that these are deficient single-nucleotide polymorphisms, as synonymous regions....
Discovering novel genes involved in immune evasion and drug resistance the human malaria parasite, Plasmodium falciparum, is of critical importance to global health. Such knowledge may assist development new effective vaccines appropriate use antimalarial drugs. By performing a full-genome scan allelic variability 14 field laboratory strains P. we comprehensively identified ≈500 evolving at higher than neutral rates. The majority most variable have paralogs within falciparum genome be...
Kinetoplast DNA (kDNA) was isolated from various species of the protozoic parasite Leishmania and analyzed by nucleic acid hybridization to detect species-related heterogeneity kDNA. Purified L. mexicana braziliensis displayed no homology in studies. These results confirmed that rapid kDNA sequence change evolution is occurring New World suggested such could be used as a specific probe for identification using whole organisms. This work further demonstrates species-specific feasible on...
We report the DNA and primary amino acid sequences of Streptomyces plicatus enzyme endo-b-N-acetylglucosaminidase H. Peptide sequence information was derived from isolated culture medium using a combination mass spectrometric methods conventional techniques, including Edman degradation carboxypeptidase Y digestion.The determined by analysis Endo-@-N-acetylglucosaminidase H gene cloned into Escherichia coli plasmid pBR322 (Robbins, P. W.
The human malaria parasite Plasmodium falciparum has a complex and multi-stage life cycle that requires extensive precise gene regulation to allow invasion hijacking of host cells, transmission, immune escape. To date, the regulatory elements orchestrating these critical processes remain largely unknown. Yet it is becoming increasingly clear long non-coding RNAs (lncRNAs) could represent missing layer across broad range organisms.To investigate capacity lncRNA in P. falciparum, we harvested...
Mosquitoes in the Anopheles gambiae complex show rapid ecological and behavioral diversification, traits that promote malaria transmission complicate vector control efforts. A high-density, genome-wide mosquito SNP-genotyping array allowed mapping of genomic differentiation between populations species exhibit varying levels reproductive isolation. Regions near centromeres or within polymorphic inversions exhibited greatest genetic divergence, but divergence was also observed elsewhere...
ABSTRACT New therapeutic agents for the treatment of malaria, world's most deadly parasitic disease, are urgently needed. Malaria afflicts 300 to 500 million people and results in 1 2 deaths annually, more than 85% all malaria-related mortality involves young children pregnant women sub-Saharan Africa. The emergence multidrug-resistant parasites, especially Plasmodium falciparum , has eroded efficacy almost currently available agents. discovery new drugs, including drugs with novel cellular...
A number of recent malaria studies have used identity by descent (IBD) to study epidemiological processes relevant control. In this paper, a software package, hmmIBD, is introduced for estimating pairwise IBD between haploid genomes, such as those the parasite, sampled from one or two populations. Source code freely available.The performance hmmIBD was verified using simulated data and benchmarked against an existing method detecting within Code all tests available. The utility across...