A5031175225- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Amoebic Infections and Treatments
- DNA Repair Mechanisms
- Parasites and Host Interactions
- Insect Resistance and Genetics
- Complement system in diseases
- HIV-related health complications and treatments
- Biochemical and Molecular Research
- Drug Transport and Resistance Mechanisms
- CRISPR and Genetic Engineering
- HIV/AIDS drug development and treatment
- Malaria Research and Control
- Hematological disorders and diagnostics
Wellcome Centre for Molecular Parasitology
2017
University of Glasgow
2017
Centre hospitalier universitaire de Québec
2014-2016
Centre hospitalier de l'Université Laval
2014-2016
Transmission represents a population bottleneck in the Plasmodium life cycle and key intervention target of ongoing efforts to eradicate malaria. Sexual differentiation is essential for this process, as only sexual parasites, called gametocytes, are infective mosquito vector. Gametocyte production rates vary depending on environmental conditions, but external stimuli remain obscure. Here, we show that host-derived lipid lysophosphatidylcholine (LysoPC) controls P. falciparum cell fate by...
<ns4:p><ns4:italic>Leishmania</ns4:italic>has a plastic genome, and drug pressure can select for gene copy number variation (CNV). CNVs apply either to whole chromosomes, leading aneuploidy, or specific genomic regions. For the latter, amplification of chromosomal regions occurs at level homologous direct inverted repeated sequences extrachromosomal circular linear amplified DNAs. This ability of<ns4:italic>Leishmania</ns4:italic>to respond by has led development screens such as Cos-Seq,...
Antimony resistance complicates the treatment of infections caused by parasite Leishmania.Using next generation sequencing, we sequenced genome four independent Leishmania guyanensis antimony-resistant (SbR) mutants and found different chromosomal alterations including aneuploidy, intrachromosomal gene amplification deletion. A segment covering 30 genes on chromosome 19 was amplified intrachromosomally in three mutants. The coding for multidrug associated protein involved antimony also...
The parasite Leishmania often relies on gene rearrangements to survive stressful environments. However, safeguarding a minimum level of genome integrity is important for cell survival. We hypothesized that maintenance genomic in would imply leading role the MRE11 and RAD50 proteins considering their DNA repair, chromosomal organization protection chromosomes ends other organisms. Attempts generate null mutants wild-type background failed we provide evidence this essential. Remarkably,...
Extrachromosomal DNA amplification is frequent in the protozoan parasite Leishmania selected for drug resistance. The extrachromosomal amplified either circular or linear, and formed at level of direct inverted homologous repeated sequences that abound genome. RAD51 recombinase plays an important role amplicons formation, but mechanism by which linear are unknown. We hypothesized infantum repair protein MRE11 required following rearrangements repeats. purified LiMRE11 showed both binding...