A5030676250- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis and Biological Evaluation
- Fullerene Chemistry and Applications
- Garlic and Onion Studies
- Trypanosoma species research and implications
- Crystallography and molecular interactions
- Pharmacological Effects of Natural Compounds
- Metal complexes synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Free Radicals and Antioxidants
- Bioactive Compounds and Antitumor Agents
- Insect Pest Control Strategies
- Medical Malpractice and Liability Issues
- Patient Safety and Medication Errors
- Vitamin C and Antioxidants Research
- Analytical chemistry methods development
- Ion Channels and Receptors
- Plant Toxicity and Pharmacological Properties
- Botanical Research and Chemistry
- Therapeutic Uses of Natural Elements
- Electrochemical Analysis and Applications
- Sesquiterpenes and Asteraceae Studies
Universidade Federal de Minas Gerais
2012-2020
Fundação Hospitalar do Estado de Minas Gerais
2017
Universidade Federal de Ouro Preto
2010-2013
Antimony resistance complicates the treatment of infections caused by parasite Leishmania.Using next generation sequencing, we sequenced genome four independent Leishmania guyanensis antimony-resistant (SbR) mutants and found different chromosomal alterations including aneuploidy, intrachromosomal gene amplification deletion. A segment covering 30 genes on chromosome 19 was amplified intrachromosomally in three mutants. The coding for multidrug associated protein involved antimony also...
Pentavalent antimonial drugs such as meglumine antimoniate (Glucantime [Glu; Sanofi-Aventis, São Paulo, Brazil]) produce severe side effects, including cardiotoxicity and hepatotoxicity, during the treatment of leishmaniasis. We evaluated role residual Sb(III) in hepatotoxicity antimoniate, well protective effect antioxidant ascorbic acid (AA) chemotherapy a murine model visceral BALB/c mice infected with Leishmania infantum were treated intraperitoneally at 80 mg Sb/kg/day commercial (Glu)...
Two novel trivalent antimony(III) and bismuth(III) complexes with the nitrogen-donor heterocyclic ligand dipyrido[3,2-a:2',3'-c]phenazine (dppz) were synthesized characterized as [Sb(dppz)Cl3]∙H2O∙CH3OH [Bi(dppz)Cl3]. The crystal structure of Sb(III) complex was determined by X-ray crystallography. These evaluated for their activity against promastigote form Sb(III)-sensitive –resistant Leishmania infantum chagasi amazonensis strains. Both more effective than dppz alone in inhibiting growth...
ATP-binding cassette (ABC) transporters have been associated with drug resistance in various diseases. The MRPA gene, a transporter of ABCC subfamily, is involved the by sequestering metal-thiol conjugates intracellular vesicles Leishmania parasite. In this study, we performed molecular characterization transporter, analysis P-glycoprotein (Pgp) and aquaglyceroporin-1 (AQP1) expression, determination antimony level antimony-susceptible -resistant lines L. (V.) guyanensis, (L.) amazonensis,...
Abstract Antimonial drugs have been used for a century in the therapy of parasitic disease leishmaniasis. Even though pentavalent antimonials are still first-line drugs, they exhibit several limitations, including severe side effects, need daily parenteral administration and drug resistance. The molecular structure antimonials, their metabolism mechanism action, being investigated. Previous studies suggest that antimony acts as prodrug which is converted to active more toxic trivalent...
Visceral leishmaniasis (VL) is a systemic parasitic disease that leads to high rates of morbidity and mortality in humans worldwide. There great need develop new drugs novel strategies make chemotherapy for this more efficacious well tolerated. Recent reports on the immunomodulatory effects low toxicity spherical carbon nanostructure fullerol led us investigate vitro vivo antileishmanial activity free encapsulated forms liposomes. When assayed against intramacrophagic Leishmania amastigotes,...
Trivalent antimonial drugs, including tartar emetic (TA), are known to induce important cardiotoxicity observed by electrocardiographic abnormalities. Liposome encapsulation was found reduce the overall acute toxicity of TA. The present work investigated cardiovascular parameters alterations rats submitted treatment with free and encapsulated TA in long-circulating liposomes. Liposomes were made using lipids DSPC, DSPE-PEG cholesterol. signals, electrocardiogram (ECG) arterial blood pressure...
The growing resistance of leishmaniasis to first-line drugs like antimonials in some regions limits the control this parasitic disease. precise mechanisms involved Leishmania antimony are still subject debate. reduction intracellular SbIII accumulation is a common change observed both laboratory-selected and field isolated resistant strains, but exact transport pathways have not yet been elucidated. In order functionally characterize routes responsible for resistance, we performed systematic...
Arsenic and antimony compounds are used to treat endemic diseases, such as cancer, leishmaniasis, schistosomiasis, in spite of their toxicity. Several studies seeking the development characterization nanocarrier systems liposomes being carried out with aim developing new drug delivery minimizing toxicity these drugs. However, lack reference methods quantify semimetals within a liposomal matrix hinders QC formulations. Therefore, validation an analytical method for arsenic quantification by...