A5023262714- Malaria Research and Control
- Mosquito-borne diseases and control
- Hemoglobinopathies and Related Disorders
- Research on Leishmaniasis Studies
- Invertebrate Immune Response Mechanisms
- Parasites and Host Interactions
- Antimicrobial Resistance in Staphylococcus
- Vector-borne infectious diseases
- Neonatal Health and Biochemistry
- Global Maternal and Child Health
- Antibiotic Use and Resistance
- Viral Infections and Outbreaks Research
- Viral Infections and Immunology Research
- Parasitic Diseases Research and Treatment
- Peripheral Neuropathies and Disorders
- Pharmacological Effects and Toxicity Studies
- Hepatitis B Virus Studies
- Infectious Encephalopathies and Encephalitis
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Methemoglobinemia and Tumor Lysis Syndrome
- Bacterial Identification and Susceptibility Testing
- Drug-Induced Hepatotoxicity and Protection
Radboud University Medical Center
2011-2022
Radboud University Nijmegen
2011-2022
Rijnstate Hospital
2020-2021
University Medical Center
2014-2018
A detailed understanding of the human infectious reservoir is essential for improving malaria transmission-reducing interventions. Here we report a multi-regional assessment population-wide transmission potential based on 1209 mosquito feeding assays in endemic areas Burkina Faso and Kenya. Across both sites, identified 39 individuals. In high endemicity settings, individuals were identifiable by research-grade microscopy (92.6%; 25/27), whilst one three lowest setting was detected molecular...
Volunteers immunized under chloroquine chemoprophylaxis with Plasmodium falciparum sporozoites (CPS) develop complete, long-lasting protection against homologous sporozoite challenge. Chloroquine affects neither nor liver-stages, but kills only asexual forms in erythrocytes once released from the liver into circulation. Consequently, CPS immunization exposes host to antigens both preerythrocytic and blood stages, induced immunity might target either of these stages. We therefore explored...
Controlled human malaria infection with sporozoites is a standardized and powerful tool for evaluation of vaccine drug efficacy but so far only applied by exposure to bites Plasmodium falciparum (Pf)-infected mosquitoes. We assessed in an open label Phase 1 trial, after intradermal injection respectively 2,500, 10,000, or 25,000 aseptic, purified, vialed, cryopreserved Pf (PfSPZ) three groups ( N = 6/group) healthy Dutch volunteers. Infection was safe parasitemia developed 15 18 volunteers...
Artemisinin combination therapy effectively clears asexual malaria parasites and immature gametocytes but does not prevent posttreatment transmission. Ivermectin (IVM) may reduce transmission by killing mosquitoes that take blood meals from IVM-treated humans.In this double-blind, placebo-controlled trial, 120 asymptomatic Plasmodium falciparum parasite carriers were randomized to receive artemether-lumefantrine (AL) plus placebo or AL a single repeated dose (200 µg/kg) of ivermectin...
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Immunization of volunteers under chloroquine prophylaxis by bites Plasmodium falciparum sporozoite (PfSPZ)–infected mosquitoes induces > 90% protection against controlled human malaria infection (CHMI). We studied intradermal immunization with cryopreserved, infectious PfSPZ in taking (PfSPZ chemoprophylaxis vaccine [CVac]). Vaccine groups 1 and 3 received 3× monthly immunizations 7.5 × 10 4 PfSPZ. Control 2 normal saline. Groups underwent CHMI (#1) mosquito bite 60 days after the third...
Immunization of healthy volunteers during receipt chemoprophylaxis with Plasmodium falciparum sporozoites (CPS-immunization) induces sterile protection from malaria. Antibody responses have long been known to contribute naturally acquired immunity against malaria, but their association after whole sporozoite immunization is not well established. We therefore studied the induction and kinetics malaria parasite antigen-specific antibodies memory B-cells (MBCs) CPS-immunization correlation...
Immunization of healthy volunteers with chloroquine ChemoProphylaxis and Sporozoites (CPS-CQ) efficiently reproducibly induces dose-dependent long-lasting protection against homologous Plasmodium falciparum challenge. Here, we studied whether can be replaced by mefloquine, which is the only other licensed anti-malarial chemoprophylactic drug that does not affect pre-erythrocytic stages, exposure to considered essential for induction CPS immunization. In a double blind randomized controlled...
Abstract Background Patterns of decreasing malaria transmission intensity make presumptive treatment an unjustifiable approach in many African settings. The controlled use anti-malarials after laboratory confirmed diagnosis is preferable low endemic areas. Diagnosis may be facilitated by rapid diagnostic tests (RDTs). In this study, the impact a government policy change, comprising provision RDTs and advice to restrict anti-malarial RDT-positive individuals, was assessed describing behaviour...
We assessed the impact of exposure to Plasmodium falciparum on parasite kinetics, clinical symptoms, and functional immunity after controlled human malaria infection (CHMI) in 2 cohorts with different levels previous malarial exposure.
A 23-year-old healthy male volunteer took part in a clinical trial which the chloroquine chemoprophylaxis and received three intradermal doses at four-week intervals of aseptic, purified Plasmodium falciparum sporozoites to induce protective immunity against malaria. Fifty-nine days after last administration 32 dose underwent controlled human malaria infection (CHMI) by bites five P. falciparum-infected mosquitoes. Eleven post-CHMI thick blood smear was positive (6 falciparum/μL blood)...
Background Primaquine (PQ) actively clears mature Plasmodium falciparum gametocytes but in glucose-6-phosphate dehydrogenase deficient (G6PDd) individuals can cause hemolysis. We assessed the safety of low-dose PQ combination with artemether-lumefantrine (AL) or dihydroartemisinin-piperaquine (DP) G6PDd African males asymptomatic P. malaria. Methods and findings In Burkina Faso, adult were randomized to treatment AL alone (n = 10) at 0.25 20) 0.40 mg/kg dosage; G6PD-normal received plus PQ....
Background: The Fleming Fund is a UK Overseas Development Aid programme aiming to improve Antimicrobial Resistance (AMR) surveillance in low-and middle-income countries Africa and Asia. has placed Country Grants over 20 countries, support implementation of the component country AMR National Action Plans. provide funding technical assistance develop governance systems, capacity for bacteriology diagnostics, reporting data analysis. In rolling out programme, we have accumulated substantial...
Primaquine (PQ) is an antimalarial drug with the potential to reduce malaria transmission due its capacity clear mature Plasmodium falciparum gametocytes in human host. However, large-scale roll-out of PQ has be counterbalanced by additional risk drug-induced hemolysis individuals suffering from Glucose-6-phospate dehydrogenase (G6PD) deficiency, a genetic condition determined polymorphisms on X-linked G6PD gene. Most studies deficiency and PQ-associated focused A- variant, combination two...
This case of a pregnant refugee from Eritrea supports the evidence that chloroquine prophylaxis is suitable for suppressing relapses P. vivax until radical cure with primaquine possible.