A5044626058- Mosquito-borne diseases and control
- Malaria Research and Control
- RNA and protein synthesis mechanisms
- Pesticide Residue Analysis and Safety
- Drug Solubulity and Delivery Systems
- Thermal and Kinetic Analysis
- Amino Acid Enzymes and Metabolism
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- Viral Infections and Vectors
- Protein Structure and Dynamics
- Chemical Safety and Risk Management
- Insect symbiosis and bacterial influences
- HIV Research and Treatment
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Parasites and Host Interactions
- Orthopedic Infections and Treatments
- Trypanosoma species research and implications
- Pharmacological Effects of Natural Compounds
- Pharmaceutical and Antibiotic Environmental Impacts
- Analytical Methods in Pharmaceuticals
- Biopolymer Synthesis and Applications
- Advanced Drug Delivery Systems
- Water Quality Monitoring and Analysis
National Institute of Environmental Health Sciences
2024
Centre National de la Recherche Scientifique
2016-2023
Université Paris Cité
2021-2023
Institut Pasteur
2017-2023
Moderna Therapeutics (United States)
2022
Gandhi Medical College & Hospital
2022
International Centre for Genetic Engineering and Biotechnology
2009-2019
Institut de Virologie
2017
International Centre for Genetic Engineering and Biotechnology
2011-2014
Pacific Northwest National Laboratory
1995
Malaria and cryptosporidiosis, caused by apicomplexan parasites, remain major drivers of global child mortality. New drugs for the treatment malaria in particular, are high priority; however, there few chemically validated targets. The natural product cladosporin is active against blood- liver-stage Plasmodium falciparum Cryptosporidium parvum cell-culture studies. Target deconvolution P. has shown that inhibits lysyl-tRNA synthetase ( Pf KRS1). Here, we report identification a series...
A problem in the search for an efficient vaccine against dengue virus is immunodominance of fusion loop epitope (FLE), a segment envelope protein E that buried at interface dimers coating mature viral particles. Anti-FLE antibodies are broadly cross-reactive but poorly neutralizing, displaying strong infection enhancing potential. FLE exposure takes place via dynamic 'breathing' virion surface. In contrast, targeting dimer (EDE), readily exposed over region conserved loop, very potent and...
The human monoclonal antibody C10 exhibits extraordinary cross-reactivity, potently neutralizing Zika virus (ZIKV) and the four serotypes of dengue (DENV1-DENV4). Here we describe a comparative structure-function analysis bound to envelope (E) protein dimers five viruses it neutralizes. We demonstrate that Fab has high affinity for ZIKV DENV1 but not DENV2, DENV3, DENV4. further show interaction with latter requires an E conformational landscape limits binding only one three independent...
The Plasmodium falciparum protein translation enzymes aminoacyl-tRNA synthetases (aaRSs) are an emergent family of drug targets. aaRS ensemble catalyses transfer amino acids to cognate tRNAs, thus providing charged tRNAs for ribosomal consumption. P. proteome expression relies on a total 36 aaRSs the three translationally independent compartments cytoplasm, apicoplast and mitochondria. In present study, we show that, this set 36, single genomic copy mitochondrial phenylalanyl-tRNA synthetase...
Accuracy of aminoacylation is dependent on maintaining fidelity during attachment amino acids to cognate tRNAs. Cis- and trans-editing protein factors impose quality control translation 8 36 Plasmodium falciparum aminoacyl-tRNA synthetase (aaRS) assemblies contain canonical putative editing modules. Based expression localization profiles these aaRSs, we propose an asymmetric distribution between the parasite cytoplasm its apicoplast editing-domain containing aaRSs. We also show that single...
Thioredoxins are vital components of Plasmodium proteome and act as both reducing agents protein disulfide reductases. The malaria parasite P. falciparum thioredoxin-2 (PfTrx-2) is part the multi-protein complex embedded within parasitophorous vacuolar membrane (PVM) which purportedly directs secretion. We have characterized structural enzymatic features PfTrx-2, we show that PfTrx-2 adopts a canonical thioredoxin fold but with significant differences in its N-terminus. Our confocal...
Helminth parasites are an assemblage of two major phyla nematodes (also known as roundworms) and platyhelminths called flatworms). These a human health burden, infections caused by helminths considered under neglected tropical diseases (NTDs). typified limited clinical treatment options threat drug resistance. Aminoacyl-tRNA synthetases (aaRSs) vital enzymes that decode genetic information enable protein translation. The specific inhibition pathogen aaRSs bores well for development next...
Specific activation of amino acids by aminoacyl-tRNA synthetases (aaRSs) is essential for maintaining fidelity during protein translation. Here, we present crystal structure malaria parasite Plasmodium falciparum tryptophanyl-tRNA synthetase (Pf-WRS) catalytic domain (AAD) at 2.6 Å resolution in complex with L-tryptophan. Confocal microscopy-based localization data suggest cytoplasmic residency this protein. Pf-WRS has an unusual N-terminal extension AlaX-like (AXD) along linker regions...
Formulation, Optimization and Evaluation of Atorvastatin Calcium Loaded Microemulsion calcium is a HMG-CoA inhibitor having an Antihyperlipidemic effect. It belongs to the II class BCS classification hence formulating microemulsion will increase its solubility/ dissolution thus improves oral bioavailability. formulation prepared by water titration method using Labrafil M1944CS, Cremophor RH 40 Transcutol HP as oil, surfactant co-surfactant respectively. A single isotropic region, which...
Abstract Malaria symptoms are driven by periodic multiplication cycles of Plasmodium parasites in human red blood corpuscles (RBCs). infection still accounts for ~600,000 annual deaths and hence discovery both new drug targets drugs remains vital. In the present study, we have investigated malaria parasite enzyme diadenosine tetraphosphate (Ap4A) hydrolase that regulates levels signalling molecules like Ap4A hydrolyzing them to ATP AMP. We tracked spatial distribution parasitic infected RBCs...
Parasitic diseases caused by eukaryotic pathogens impose significant health and economic burden worldwide. The level of research funding available for many parasitic is insufficient in relation to their adverse social impact. In this article, we discuss that extant 3D structural data on protein–inhibitor complexes can be harnessed accelerate drug discovery against related pathogens. Assessment sequence conservation within drug/inhibitor-binding residues enzyme–inhibitor leveraged predict...
A post-screening classification assay for tetracycline compounds has been developed and integrated into the previously reported optimized PremiTest methodology. The new post-antimicrobial screening is based on a metal ion chelation using calcium sodium chloride shown to be specific towards class. both cost-effective complementary procedures that have beta-lactam sulfonamide compounds. validation study was conducted in accordance with 2002/657/EC (Commission Decision). method rugged...
All enveloped viruses enter cells by fusing their envelope with a target cell membrane while avoiding premature fusion membranes of the producer cell-the latter being particularly important for that bud at internal membranes. Flaviviruses in endoplasmic reticulum, are transported through TGN to reach external milieu, and other via receptor-mediated endocytosis. The trigger is acidic environment early endosomes, which has similar pH cell. viral particles therefore become activated react...