A5010850019- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Malaria Research and Control
- Biochemical and Molecular Research
- Synthesis and Biological Evaluation
- Synthesis and biological activity
- Parasites and Host Interactions
- Plant Stress Responses and Tolerance
- HIV/AIDS drug development and treatment
- Phenothiazines and Benzothiazines Synthesis and Activities
- Lysosomal Storage Disorders Research
- Computational Drug Discovery Methods
- Insect symbiosis and bacterial influences
- Calcium signaling and nucleotide metabolism
- Parasite Biology and Host Interactions
- Plant nutrient uptake and metabolism
- Helminth infection and control
- Bioactive Compounds and Antitumor Agents
- Traditional and Medicinal Uses of Annonaceae
- Quinazolinone synthesis and applications
- Plant Molecular Biology Research
- Phytochemical compounds biological activities
- Insect Pest Control Strategies
- Mosquito-borne diseases and control
- Microbial Natural Products and Biosynthesis
Swiss Tropical and Public Health Institute
2016-2025
University of Basel
2016-2025
University Hospital of Geneva
2024
Centre universitaire de médecine générale et santé publique, Lausanne
2024
HES-SO University of Applied Sciences and Arts Western Switzerland
2024
University of Lausanne
2024
University of Geneva
2024
University of Bern
2003-2015
University of Glasgow
2011-2012
University of Al Jabal Al Gharbi
2012
Abstract Uptake and translocation of cationic nutrients play essential roles in physiological processes including plant growth, nutrition, signal transduction, development. Approximately 5% the Arabidopsis genome appears to encode membrane transport proteins. These proteins are classified 46 unique families containing approximately 880 members. In addition, several hundred putative transporters have not yet been assigned families. this paper, we analyzed phylogenetic relationships over 150...
Potassium is an important macronutrient and the most abundant cation in plants. Because soil mineral conditions can vary, plants must be able to adjust different nutrient availabilities. Here, we used Affymetrix Genechip microarrays identify genes responsive potassium (K(+)) deprivation roots of mature Arabidopsis (Arabidopsis thaliana) Unexpectedly, only a few were changed their expression level after 6, 48, 96 h K(+) starvation even though root content was reduced by approximately 60%....
Sodium (Na + ) is toxic to most plants, but the molecular mechanisms of plant Na uptake and distribution remain largely unknown. Here we analyze Arabidopsis lines disrupted in transporter AtHKT1 . expressed root stele leaf vasculature. athkt1 null plants exhibit lower levels are more salt resistant than wild‐type short‐term growth assays. In shoot tissues, however, disruption produces higher levels, / sos3 shoots ‐hypersensitive long‐term Thus controls root/shoot counteracts stress leaves by...
Theileria annulata and T. parva are closely related protozoan parasites that cause lymphoproliferative diseases of cattle. We sequenced the genome compared it with to understand mechanisms underlying transformation tropism. Despite high conservation gene sequences synteny, analysis reveals unequally expanded families species-specific genes. also identify divergent putative secreted polypeptides may reduce immune recognition, candidate regulators host-cell transformation, a Theileria-specific...
Plant HKT proteins comprise a family of cation transporters together with prokaryotic KtrB, TrkH, and KdpA transporter subunits fungal Trk proteins. These contain four loop domains in one polypeptide proposed distant homology to K + channel selectivity filters. Functional expression yeast Xenopus oocytes revealed that wheat HKT1 mediates Na -coupled transport. Arabidopsis AtHKT1, however, transports only eukaryotic systems. To understand the molecular basis this difference we constructed...
Motivation: Anchoring of proteins to the extracytosolic leaflet membranes via C-terminal attachment glycosylphosphatidylinositol (GPI) is ubiquitous and essential in eukaryotes. The signal for GPI-anchoring confined C-terminus target protein. In order identify anchoring signals silico, we have trained neural networks on known GPI-anchored proteins, systematically optimizing input parameters. Results: A Kohonen self-organizing map, GPI-SOM, was developed that predicts with high accuracy....
Drug resistance of pathogens is an increasing problem whose underlying mechanisms are not fully understood. Cellular uptake the major drugs against Trypanosoma brucei spp., causative agents sleeping sickness, thought to occur through unusual, so far unidentified adenosine transporter. Saccharomyces cerevisiae was used in a functional screen clone gene ( TbAT1 ) from that encodes nucleoside When expressed yeast, enabled and conferred susceptibility melaminophenyl arsenicals. Drug-resistant...
ABSTRACT Sleeping sickness, caused by Trypanosoma brucei spp., has become resurgent in sub-Saharan Africa. Moreover, there is an alarming increase treatment failures with melarsoprol, the principal agent used against late-stage sleeping sickness. In T. , uptake of melarsoprol as well diamidines thought to be mediated P2 aminopurine transporter, and loss function been implicated resistance these agents. The trypanosomal gene TbAT1 found encode a P2-type transporter when expressed yeast. Here...
The heartworm Dirofilaria immitis is an important parasite of dogs. Transmitted by mosquitoes in warmer climatic zones, it spreading across southern Europe and the Americas at alarming pace. There no vaccine, chemotherapy prone to complications. To learn more about this parasite, we have sequenced genomes D. its endosymbiont Wolbachia. We predict 10,179 protein coding genes 84.2 Mb nuclear genome, 823 0.9-Mb Wolbachia genome. genome harbors neither DNA transposons nor active...
Malaria and cryptosporidiosis, caused by apicomplexan parasites, remain major drivers of global child mortality. New drugs for the treatment malaria in particular, are high priority; however, there few chemically validated targets. The natural product cladosporin is active against blood- liver-stage Plasmodium falciparum Cryptosporidium parvum cell-culture studies. Target deconvolution P. has shown that inhibits lysyl-tRNA synthetase ( Pf KRS1). Here, we report identification a series...
Trypanosoma brucei drug transporters include the TbAT1/P2 aminopurine transporter and high-affinity pentamidine (HAPT1), but genetic identity of HAPT1 is unknown. We recently reported that loss T. aquaglyceroporin 2 (TbAQP2) caused melarsoprol/pentamidine cross-resistance (MPXR) in these parasites current study aims to delineate mechanism by which this occurs. The TbAQP2 loci isogenic pairs drug-susceptible MPXR strains subspecies were sequenced. Drug susceptibility profiles trypanosome...
Neglected tropical diseases cause significant morbidity and mortality are a source of poverty in endemic countries. Only few drugs available to treat such as leishmaniasis, Chagas’ disease, human African trypanosomiasis malaria. Since drug development is lengthy expensive, repurposing strategy offers an attractive fast-track approach speed up the process. A set 100 registered with repositioning potential for neglected was assembled tested vitro against four protozoan parasites associated...
Kinetoplastid parasites have caused human disease for millennia. Significant achievements been made toward developing new treatments leishmaniasis (particularly on the Indian subcontinent) and African trypanosomiasis (HAT). Moreover, sustained decrease in incidence of HAT has prospect elimination a tantalizing reality. Despite gains, no chemical or biological entities to treat kinetoplastid diseases registered more than three decades, work is needed discover safe effective therapies patients...
Millions who live in Latin America and sub-Saharan Africa are at risk of trypanosomatid infections, which cause Chagas disease human African trypanosomiasis (HAT). Improved HAT treatments available, but therapies rely on two nitroheterocycles, suffer from lengthy drug regimens safety concerns that frequent treatment discontinuation. We performed phenotypic screening against trypanosomes identified a class cyanotriazoles (CTs) with potent trypanocidal activity both vitro mouse models HAT....