A5068989093- Antibiotic Resistance in Bacteria
- Ruminant Nutrition and Digestive Physiology
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Reproductive Physiology in Livestock
- Polyomavirus and related diseases
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Biochemical and Molecular Research
- Full-Duplex Wireless Communications
- Acute Myeloid Leukemia Research
- Genetic and phenotypic traits in livestock
- Vibrio bacteria research studies
- Photodynamic Therapy Research Studies
- Lymphoma Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Synthesis and Biological Evaluation
- Growth Hormone and Insulin-like Growth Factors
- Animal health and immunology
- Antenna Design and Analysis
- Fungal Infections and Studies
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Retinoids in leukemia and cellular processes
- Chronic Lymphocytic Leukemia Research
University of Basel
2013-2025
Liverpool School of Tropical Medicine
2023-2025
University of Gothenburg
2018-2024
University of Zurich
2023
Swiss Tropical and Public Health Institute
2013-2017
Ludwig-Maximilians-Universität München
1977-2009
Semmelweis University
1986-1992
Technical University of Munich
1991
Trypanosoma brucei drug transporters include the TbAT1/P2 aminopurine transporter and high-affinity pentamidine (HAPT1), but genetic identity of HAPT1 is unknown. We recently reported that loss T. aquaglyceroporin 2 (TbAQP2) caused melarsoprol/pentamidine cross-resistance (MPXR) in these parasites current study aims to delineate mechanism by which this occurs. The TbAQP2 loci isogenic pairs drug-susceptible MPXR strains subspecies were sequenced. Drug susceptibility profiles trypanosome...
The predominant mechanism of drug resistance in African trypanosomes is decreased uptake due to loss-of-function mutations the genes for transporters that mediate import. role as determinants susceptibility well documented from laboratory-selected Trypanosoma brucei mutants. But clinical isolates, especially T. b. gambiense, are less amenable experimental investigation since they do not readily grow culture without prior adaptation. Here we analyze a selected panel 16 ssp. field isolates (i)...
Infections with Enterobacterales (E) are increasingly difficult to treat due antimicrobial resistance. After ceftriaxone replaced chloramphenicol (CHL) as empiric therapy for suspected sepsis in Malawi 2004, extended-spectrum beta-lactamase (ESBL)-E rapidly emerged. Concurrently, resistance CHL Escherichia coli and Klebsiella spp. decreased, raising the possibility of re-introduction. However, many phenotypically susceptible isolates still carry acetyltransferase (cat) genes. To understand...
Aquaglyceroporin-2 is a known determinant of melarsoprol-pentamidine cross-resistance in Trypanosoma brucei laboratory strains. Recently, chimerization at the AQP2-AQP3 tandem locus was described from cross-resistant gambiense isolates sleeping sickness patients Democratic Republic Congo. Here, we demonstrate that reintroduction wild-type AQP2 into one these fully restores drug susceptibility while expression chimeric AQP2/3 gene aqp2-aqp3 null T. b. does not. This proves cause isolates.
Immune escape contributes to viral persistence, yet little is known about human polyomaviruses. BK-polyomavirus (BKPyV) asymptomatically infects 90% of humans but causes premature allograft failure in kidney transplant patients. Despite virus-specific T cells and neutralizing antibodies, BKPyV persists kidneys evades immune control as evidenced by urinary shedding immunocompetent individuals. Here, we report that disrupts the mitochondrial network membrane potential when expressing 66aa-long...
Abstract Background Infections caused by multidrug-resistant gram-negative bacteria present a severe threat to global public health. The WHO defines drug-resistant Klebsiella pneumoniae as priority pathogen for which alternative treatments are needed given the limited treatment options and rapid acquisition of novel resistance mechanisms this species. Longitudinal descriptions genomic epidemiology can inform management strategies but data from sub-Saharan Africa lacking. Methods We...
Abstract Major antibiotic groups are losing effectiveness due to the uncontrollable spread of antimicrobial resistance (AMR) genes. Among these, β-lactam genes –encoding β-lactamases– stand as most common mechanism in Enterobacterales their frequent association with mobile genetic elements. In this context, novel approaches that counter AMR urgently needed. Collateral sensitivity (CS) occurs when acquisition one increases susceptibility another and can be exploited eliminate selectively....
Twelve second-lactation Deutsches Fleckvieh cows, (FV, a dual-purpose breed) and 12 German Black White (BW, dairy were used to evaluate similarities differences in the response prolonged-release formulation of sometribove (N-methionyl bovine somatotropin, bST). Cows milked twice daily fed basic diet (70% com silage, 30% alfalfa hay) ad libitum plus 1 kg concentrate. Additionally, concentrates supplements offered at rate for each 2.2 milk produced above kg/ d. Six cows breed treated s.c. with...
Summary Suramin is one of the first drugs developed in a medicinal chemistry program (Bayer, 1916), and it still treatment choice for hemolymphatic stage African sleeping sickness caused by Trypanosoma brucei rhodesiense . Cellular uptake suramin occurs endocytosis, reverse genetic studies with T b have linked downregulation endocytic pathway to resistance. Here we show that forward selection resistance spp. cultures fast, highly reproducible antigenic variation. Bloodstream‐form...
Background Endocrine therapy is standard of care maintenance treatment for estrogen receptor (ER) positive breast cancers and increasingly used in low-grade serous ovarian cancer. However, its therapeutic role the early setting ER high-grade cancer (HGSC) remains undefined. Methods A retrospective analysis was conducted on clinicopathological data from patients with newly diagnosed HGSC following completion adjuvant chemotherapy. Patients received either or without aromatase inhibitor...
Background: Endocrine therapy is the standard-of-care maintenance treatment for estrogen receptor (ER)-positive breast cancers and increasingly used in low-grade serous ovarian cancer. However, its therapeutic role early setting ER-positive high-grade cancer (HGSC) remains undefined. Methods: A retrospective analysis was conducted on clinicopathological data from patients with newly diagnosed HGSC following completion of adjuvant chemotherapy. Patients received either or without aromatase...
Background: The blaNDM carbapenemase gene provides Gram-negative bacteria the ability to hydrolyse almost all beta-lactam antibiotics. A 90% year on increase from 2022 2023 in prevalence of clinical isolates was identified a microbiology laboratory serving Merseyside, UK hospitals and GP practices 2023. We analysed disclose species plasmid diversity understand mechanism dissemination across Merseyside hospitals. Methods: Data regarding sample type, isolation date location samples collected...
Introduction. Resistance to the β -lactam/ -lactamase inhibitor (BL/BLI) combination antibiotic piperacillin/tazobactam (TZP) predominantly occurs via enzymes, also leading resistance third-generation cephalosporins (3GCs). However, if -lactamases inactive against 3GCs and inhibited by tazobactam are expressed at high levels, enzyme hyperproduction, surplus escapes inhibition inactivates piperacillin. Hypothesis/Gap statement. Understanding this mechanism is clinically relevant, as...
Two experiments were conducted to monitor hormonal changes during lactation in crossbred sows (PietrainX German Landrace). Sows fed twice daily without weighing the remaining food. Number of piglets was not standardized. Plasma concentrations growth hormone (GH), prolactin (PRL), insulin-like factor-1 (IGF-1), IGF-2, insulin (INS), triiodothyronine (T3), thyroxin (T4), free (FT4), non esterifíed fatty acids (NEFA) and glucose (GLUC) determined by RIA, EIA or enzymatically. In exp. A (n=5...
African trypanosomes, like all obligate parasitic protozoa, cannot synthesize purines de novo and import from their hosts to build nucleic acids. The purine salvage pathways of Trypanosoma brucei being redundant, none the involved enzymes is likely be essential. Nevertheless they can pharmacological interest due role in activation nucleobase or nucleoside analogues, which only become toxic when converted nucleotides. Aminopurine antimetabolites, particular, are potent trypanocides even...
Trypanosoma brucei rhodesiense is one of the causative agents human sleeping sickness, a fatal disease that transmitted by tsetse flies and restricted to Sub-Saharan Africa. Here we investigate two independent lines T. b. have been selected with drugs melarsoprol pentamidine over course 2 years, until they exhibited stable cross-resistance an unprecedented degree. We apply comparative genomics transcriptomics identify underlying mutations. Only few mutations become fixed during selection....
The rapid transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause the accelerating crisis. There are currently no experimental platforms for fast and cost-efficient screening genetic effects by conjugation, which prevents understanding targeting conjugation.
The prognostic value of cell differentiation antigens detected with the monoclonal antibodies VI series was studied in 242 cases acute nonlymphoblastic leukemia (ANLL) treated 7 cooperating centers. A significantly higher complete remission rate observed patients a expression CD-15 antigen by VIM-D5 antibody than those lower values. These significant differences were proved when comparing subgroups positivity blastic cells less 15 and much greater 15% (p 0.01) as well values 50% (median...
Zusammenfassung Bei 11 Holstein-Friesian-Kühen wurden im Blutplasma und in Homogenaten von Leber, Herz, Skelettmuskel, Niere, Milz, Uterus Euter die Aktivitäten GOT, LDH, CK, SDH, GlDH γ-GT sowie Isoenzyme der LDH bestimmt. Die Organenzymaktivitäten Kühe lagen bis auf wenige Ausnahmen über denen Bullen. Reihenfolge Organe nach HÖhe Enzymaktivitäten stimmte bei Kühen Bullen weitgehend überein. höchsten hatten GOT Leber Muskel, CK Skelett- Herzmuskel, SDH Niere Niere. Aus diesen Ergebnissen...
Zusammenfassung Enzymaktivitäten von GOT, LDH, CK, SDH, GIDH und γ-GT sowie die 5 Isoenzyme der LDH wurden im Blutplasma in Organhomogenaten (Leber, Niere, Herz, Milz, Skelettmuskel) 7 jungen Fleckviehbullen bestimmt eine Reihenfolge Organe bezüglich Aktivitätshöhe aufgestellt. scheinen beim Rind keine Organspezifität zu besitzen, während CK muskel- bzw. herzmuskelspezifisch SDH leberspezifisch ist. kann nach Ausschluß eines Nierenschadens ebenfalls als angesehen werden. Die...
One pertinent complication in bacterial infection is the growth of biofilms, that is, communities surface‐adhered bacteria resilient to antibiotics. Photodynamic inactivation (PDI) has been proposed as an alternative antibiotic treatment; however, novel techniques complementing standard efficacy measures are required. Herein, we present approach employing multiphoton microscopy complemented with Airyscan super‐resolution microscopy, visualize distribution curcumin Staphylococcus epidermidis...