A5073025337- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- Vibrio bacteria research studies
- Hepatitis C virus research
- Virology and Viral Diseases
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- Complement system in diseases
- Blood groups and transfusion
- Long-Term Effects of COVID-19
- Hepatitis B Virus Studies
- S100 Proteins and Annexins
- Parvovirus B19 Infection Studies
- HIV Research and Treatment
- Insect symbiosis and bacterial influences
- RNA Interference and Gene Delivery
- vaccines and immunoinformatics approaches
- Memory and Neural Mechanisms
Institut Pasteur
2011-2025
Centre National de la Recherche Scientifique
2010-2025
Université Paris Cité
2021-2025
Institut de Virologie
2013-2021
Rockefeller University
2005-2010
Inserm
1997-2005
Sorbonne Université
2005
Pitié-Salpêtrière Hospital
2005
Washington University in St. Louis
2002
Institut Necker Enfants Malades
1999
There is now abundant evidence to substantiate an important role of hepatitis C virus (HCV) core protein in cellular gene expression as well the viral cycle. Thus subcellular localization this has implications. However, several studies have shown controversial results: HCV been, indeed, described cytoplasmic or nuclear depending on size genotype analyzed. We studied two different cell lines, one nonhepatic (CHO) and other hepatic (HepG2). Double immunofluorescence staining using a membrane...
Liver steatosis, which involves accumulation of intracytoplasmic lipid droplets, is characteristic hepatitis C virus (HCV) infection. By use an in vivo transgenic murine model, we demonstrate that hepatic overexpression HCV core protein interferes with the assembly and secretion triglyceride-rich very low density lipoproteins (VLDL). Core expression led to reduction microsomal triglyceride transfer (MTP) activity particle size nascent VLDL without affecting MTP disulfide isomerase. Hepatic...
Previous research suggests that patients with Alzheimer's disease (AD) are impaired on executive function early in the course of disease, but negative findings were reported. To evaluate performance tasks AD and to determine involvement memory outcome tasks. Thirty-six divided into two subgroups basis MMSE: very mild mild. The comparison 17 normal controls shows had deficits visuospatial short-term memory, episodic flexibility self-monitoring abilities, concept formation reasoning. showed...
Neurological manifestations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection represent a major issue in long disease. How SARS-CoV-2 gains access to the brain and how leads neurological symptoms are not clear because principal means viral entry by endocytosis, angiotensin-converting enzyme receptor, barely detectable brain. We report that human neuronal cells, nonpermissive through endocytic pathway, can be infected when cocultured with permissive epithelial cells....
A comprehensive understanding of the development and evolution human B cell responses induced by pathogen exposure will facilitate design next-generation vaccines. Here, we utilized a high-throughput single cloning technology to longitudinally track response yellow fever virus 17D (YFV-17D) vaccine. The early memory (MBC) was mediated both classical immunoglobulin M (IgM) (IgM + CD27 ) switched (swIg MBC populations; however, IgM MBCs waned rapidly, whereas swIg atypical IgD were stable over...
Humans display substantial interindividual clinical variability after SARS-CoV-2 infection1-3, the genetic and immunological basis of which has begun to be deciphered4. However, extent drivers population differences in immune responses remain unclear. Here we report single-cell RNA-sequencing data for peripheral blood mononuclear cells-from 222 healthy donors diverse ancestries-that were stimulated with or influenza A virus. We show that induces weaker, but more heterogeneous,...
Several lines of evidence suggest that hepatitis C virus (HCV) core protein may modulate cellular transduction signals and alter lipid metabolism. We have investigated the binding HCV to proteins by combining 2 yeast hybrid, confocal, surface plasmon resonance assays. Our results show direct viral apolipoprotein AII (apoAII) map interaction domain C-terminal protein. To investigate biological relevance between metabolism, we took advantage well-established increase in apoAII expression...
The yellow fever (YF) 17D vaccine is one of the most successful live attenuated vaccines available. A single immunization induces both long-lasting neutralizing antibody and YF-specific T cell responses. Surprisingly, mechanism for this robust immunity has not been addressed. In light several recent reports suggesting flavivirus interaction with dendritic cells (DCs), we investigated YF17D DCs importance in generating immunity. Our results show that can infect immature mature human DCs....
Abstract Influenza A virus (IAV) infection results in a highly contagious respiratory illness leading to substantial morbidity and occasionally death. In this report, we assessed the vivo physiological contribution of invariant NKT (iNKT) lymphocytes, subset lipid-reactive αβ T on host response viral pathogenesis using virulent, mouse-adapted, IAV H3N2 strain. Upon with lethal dose IAV, iNKT cells become activated lungs bronchoalveolar space rapidly anergic further restimulation. Relative...
ABSTRACT RNA viruses present an extraordinary threat to human health, given their sudden and unpredictable appearance, the potential for rapid spread among population, ability evolve resistance antiviral therapies. The recent emergence of chikungunya virus, Zika Ebola virus highlights struggles contain outbreaks. A significant hurdle is availability antivirals treat infected or protect at-risk populations. While several compounds show promise in vitro vivo , these outbreaks underscore need...
A problem in the search for an efficient vaccine against dengue virus is immunodominance of fusion loop epitope (FLE), a segment envelope protein E that buried at interface dimers coating mature viral particles. Anti-FLE antibodies are broadly cross-reactive but poorly neutralizing, displaying strong infection enhancing potential. FLE exposure takes place via dynamic 'breathing' virion surface. In contrast, targeting dimer (EDE), readily exposed over region conserved loop, very potent and...
Abstract Arthropod-borne viruses pose a major threat to global public health. Thus, innovative strategies for their control and prevention are urgently needed. Here, we exploit the natural capacity of generate defective viral genomes (DVGs) detriment. While DVGs have been described most viruses, identifying which, if any, can be used as therapeutic agents remains challenge. We present combined experimental evolution computational approach triage DVG sequence space pinpoint fittest deletions,...
Inborn and acquired deficits of type I interferon (IFN) immunity predispose to life-threatening COVID-19 pneumonia. We longitudinally profiled the B cell response mRNA vaccination in SARS-CoV-2 naive patients with inherited TLR7, IRF7, or IFNAR1 deficiency, as well young autoantibodies neutralizing IFNs due autoimmune polyendocrine syndrome type-1 (APS-1) older individuals age-associated IFNs. The receptor-binding domain spike protein (RBD)–specific memory all was quantitatively...
The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). positive-sense single-stranded RNA virus contains a single linear segment that serves as template for transcription and replication, leading to synthesis of positive negative-stranded viral (vRNA) in infected cells. Tools visualize vRNA directly cells are critical analyze replication cycle, screen therapeutic molecules, or study infections human tissue. Here, we report design,...
Abstract The yellow fever 17D vaccine (YF17D) is highly effective but frequently administered to individuals with pre-existing cross-reactive immunity, potentially impacting their immune responses. Here, we investigate the impact of flavivirus immunity induced by tick-borne encephalitis virus (TBEV) on response YF17D vaccination in 250 up 28 days post-vaccination (pv) and 22 sampled one-year pv. Our findings indicate that previous TBEV does not affect early IgM-driven neutralizing YF17D....
T follicular helper (Tfh) cells are the principal cell subset that provides help to B for potent antibody responses against various pathogens. In this study, we took advantage of live-attenuated yellow fever virus (YFV) vaccine strain, YF-17D, as a model system studying human antiviral immune in vivo following exposure an acute primary challenge under safe and highly controlled conditions, comprehensively analyse dynamics circulating Tfh (cTfh) cells.We tracked analysed response cTfh other...