A5102891916- Inflammatory Bowel Disease
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Microscopic Colitis
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolites in Food Biotechnology
- Diet and metabolism studies
- Immunodeficiency and Autoimmune Disorders
- Effects of Radiation Exposure
- Thyroid Disorders and Treatments
- Dermatology and Skin Diseases
- Liver Disease Diagnosis and Treatment
- Cystic Fibrosis Research Advances
- NF-κB Signaling Pathways
- Thyroid Cancer Diagnosis and Treatment
- Ethics and bioethics in healthcare
- Cardiac Valve Diseases and Treatments
- Soil Moisture and Remote Sensing
- Organ Transplantation Techniques and Outcomes
- Digestive system and related health
- Immune Response and Inflammation
- Precipitation Measurement and Analysis
- Autoimmune and Inflammatory Disorders
Universitat Autònoma de Barcelona
2009-2024
Vall d'Hebron Hospital Universitari
2002-2024
Vall d'Hebron Institut de Recerca
2008-2024
Hebron University
1997-2023
Instituto de Salud Carlos III
2016-2022
Centro de Investigación Biomédica en Red
2015-2016
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2008-2015
Sorbonne Paris Cité
2014
Assistance Publique – Hôpitaux de Paris
2014
Centre d'Investigacions sobre Desertificació
2010
The intestinal microbiota consists of over 1000 species, which play key roles in gut physiology and homeostasis. Imbalances the composition this bacterial community can lead to transient dysfunctions chronic disease states. Understanding how manipulate ecosystem is thus essential for treating many disorders. In study, we took advantage recently developed tools deep sequencing phylogenetic clustering examine long-term effects exogenous transplantation combined with without an antibiotic...
Although incrimination of the intestinal microbiota in pathogenesis IBD is widely accepted, few data are available about role specific bacteria. Potentially, Faecalibacterium prausnitzii, bacteria with anti-inflammatory properties, might be deficient ulcerative colitis (UC).To quantify F. prausnitzii faecal UC patients remission and determine its relationship relapse.A cross-sectional study included 116 remission, 29 first-degree relatives 31 healthy controls. A subset eighteen patients,...
To identify factors predictive of early postoperative graft function, we analyzed 54 variables—including easily available clinical and laboratory data prospectively obtained from organ donors, transplant recipients surgical procedures in 168 consecutive liver transplantations. Early function was classified into three groups according to a scoring system ranging 3 9 based on peak serum ALT values, mean bile output lowest prothrombin activity measured during the 72 hr after transplant: group 1...
Summary Background Inulin and oligofructose promote selective growth of saccharolytic bacteria with low inflammatory potential. Objective To test the effect oligofructose‐enriched inulin in patients active ulcerative colitis. Design Prospective, randomized, placebo controlled pilot trial. Eligible had been previously remission mesalazine as maintenance therapy or no drug, presented a relapse mild to moderate activity. They were treated (3 g/day) randomly allocated receive either (12 g/day,...
Inulin stimulates intracolonic generation of butyrate and growth lactic acid bacteria. This study investigated whether inulin protects against colitis.Rats with dextran sodium sulfate colitis received either orally (1% in drinking water, or 400 mg/day) by enema. Matched groups vehicle. In addition, fecal water obtained from inulin-fed rats was administered enema to compared control rats. Finally, daily enemas (at 40 80 mmol/L) Inflammation assessed eicosanoid asssay rectal dialysates MPO...
Diarrhea-predominant irritable bowel syndrome (IBS-D) patients show altered epithelial permeability and mucosal micro-inflammation in both proximal distal regions of the intestine. The objective this study was to determine molecular events mechanisms clinical role upper small intestinal alterations.Clinical assessment a jejunal biopsy obtained IBS-D healthy subjects. Routine histology immunohistochemistry performed all participants assess number mast cells (MCs) intraepithelial lymphocytes....
BACKGROUND AND AIM Studies have identified abnormal characteristics of the gut microbiota in patients with active IBD, but whether changes are causal or secondary to inflammation remains uncertain. We investigated dynamics fecal ulcerative colitis (UC) during remission by genomic technology. PATIENTS METHODS Patients clinical and on stable maintenance mesalazine therapy were recruited (N = 33). Fecal samples collected at regular intervals over a period 1 yr. Sixteen who remained eight...
INTRODUCTION Severe diarrhea may complicate pelvic radiotherapy and force interruption of treatment. As there is no current clinical or experimental information on the role gut microbiota in this pathogenesis, we conducted a pilot observational study fecal patients receiving radiotherapy. METHODS The involved 10 who underwent 5 wk for abdominal tumors controls. Four samples were collected from each individual: before, during, at end, 2 after Following amplification bacterial 16S rRNA gene...
The interaction of commensal bacteria with the intestinal immune system is an essential factor in development inflammatory bowel disease (IBD). study isolated bacteria's effects on mucosal response might be relevant for a better understanding pathophysiological mechanisms IBD.We investigated responses to signals from Escherichia coli ATCC 35345 and probiotic Lactobacillus casei DN-114 001 Crohn's (CD) mucosa. Ileal specimens were obtained during surgery CD patients. Mucosal explants...
The influence of microbiota in human health is well known. Imbalances microbiome structure have been linked to several diseases. Modulation composition through probiotic therapy an attempt harness the beneficial effects commensal microbiota. Although there wide knowledge responses induced by gut microbiota, microbial factors that mediate these are not Gram-negative bacteria release outer membrane vesicles (OMVs) as a secretion mechanism factors, which important role intercellular...
Bacteria and their products stimulate inflammatory responses. The effects of different antimicrobial regimens (amoxicillin/clavulanic acid, tobramycin, imipenem, vancomycin, metronidazole) were investigated on the course experimental colitis induced by trinitrobenzenesulphonic acid (TNB) in rat. On day 7 21 after induction colitis, matched groups control antibiotic treated rats subjected to colonic dialysis measure eicosanoid release, killed for morphological assessment lesions (macro...
Commensal bacteria may participate in the pathogenesis of bowel inflammation. We studied role from rat colonic flora on transmural inflammation induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). First, bacterial translocation to wall after induction colitis was assessed microbiological and histological methods. Second, rats with a segment excluded fecal transit were prepared for recolonization preselected used test effects different species (eicosanoid release, tissue myeloperoxidase)...
The inflammatory activity of colonic mucosal lesions may be stimulated by intraluminal bacteria. Our aim was to investigate whether administration broad-spectrum antibiotics decreases in ulcerative colitis. To this end, we performed a randomized, 5-day study with either oral enterically coated amoxicillin-clavulanic acid (1 g + 250 mg, t.i.d.); i.v. methylprednisolone (40 mg/day) and placebo (t.i.d.); or both as above, 30 patients clinically active Before after 5 days treatment, intestinal...
Matrix metalloproteinases may play a role in tissue remodelling and destruction associated with inflammation. We investigated activity expression of matrix rat model colitis tested the therapeutic potential synthetic inhibitor (CGS-27023-A). Colitis was induced by dextran sulphate sodium (at 5% drinking water for 5 days) group eight rats, whereas matched control received plain water. Activity were measured colonic homogenates using zymography Western blot on days 3 after induction colitis....
Abstract Apoptosis of active T lymphocytes constitutes a maor control mechanism immune homeostasis and tolerance. In Crohn's disease, abnormal activation mucosal against enteric bacteria is the key event triggering intestinal inflammation. Resistance to apoptosis has been proposed as pathogenetic defect. We examined influence bacteria-mucosa interactions on lymphocytes. Ileal specimens were obtained at surgery from 12 patients with disease. Mucosal explants each specimen cultured...
Exopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many their roles not yet elucidated. We sequenced the genomes two isogenic strains Bifidobacterium animalis subsp. lactis that differ in EPS-producing phenotype. The original strain displays nonmucoid appearance, and mutant derived thereof has acquired mucoid sequence analysis revealed nonsynonymous mutation gene...
The present study was designed to investigate the prevalence of bacterial overgrowth in patients with exocrine pancreatic insufficiency by using hydrogen breath test glucose. Thus, 30 (in 15 due chronic pancreatitis and associated primary immunodeficiency), established quantifying trypsin output before after stimulation cerulein a duodenal perfusion technique, glucose performed administering 50 g H2 gas chromatography. positive six but only one immunodeficient (p < 0.05). Age, sex, etiology,...
Abstract. The main goal of the SMOS (Soil Moisture and Ocean Salinity) mission is to deliver global fields surface soil moisture sea salinity using L-band (1.4 GHz) radiometry. Within context Science preparation for SMOS, Valencia Anchor Station (VAS) experimental site, in Spain, was chosen be one test sites Europe Calibration/Validation (Cal/Val) activities. In this framework, paper presents an approach consisting accurately simulating a whole pixel by representing spatial temporal...