A5033216871- Gastrointestinal motility and disorders
- Gut microbiota and health
- Dermatology and Skin Diseases
- Helicobacter pylori-related gastroenterology studies
- Diet and metabolism studies
- Eosinophilic Esophagitis
- Barrier Structure and Function Studies
- Celiac Disease Research and Management
- Digestive system and related health
- Transgenic Plants and Applications
- Biliary and Gastrointestinal Fistulas
- Gastrointestinal disorders and treatments
- Stress Responses and Cortisol
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- Congenital gastrointestinal and neural anomalies
- Microbial Metabolites in Food Biotechnology
- Infant Health and Development
- Food Allergy and Anaphylaxis Research
- Omental and Epiploic Conditions
- Music Therapy and Health
- 14-3-3 protein interactions
- IL-33, ST2, and ILC Pathways
- Medical research and treatments
- Cannabis and Cannabinoid Research
Vall d'Hebron Institut de Recerca
2010-2025
Universitat Autònoma de Barcelona
2010-2025
Vall d'Hebron Hospital Universitari
2012-2025
McMaster University
2014-2022
Population Health Research Institute
2015-2022
Juravinski Hospital
2018
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2010-2017
Université de Sherbrooke
2016
Fecal microbiota transplants from patients with irritable bowel syndrome and anxiety alter gut function behavior in germ-free mice.
<h3>Objective</h3> Recently, the authors demonstrated altered gene expression in jejunal mucosa of diarrhoea-predominant irritable bowel syndrome patients (IBS-D); specifically, showed that genes related to mast cells and intercellular apical junction complex (AJC) were expressed differently than healthy subjects. The aim here was determine whether these alterations are associated with structural abnormalities AJC their relationship cell activation IBS-D clinical manifestations....
The gut microbiota has been implicated in chronic pain disorders, including irritable bowel syndrome (IBS), yet specific pathophysiological mechanisms remain unclear. We showed that decreasing intake of fermentable carbohydrates improved abdominal patients with IBS, and this was accompanied by changes the decreased urinary histamine concentrations. Here, we used germ-free mice colonized fecal from IBS to investigate role bacteria neuroactive mediator visceral hypersensitivity. Germ-free who...
Diarrhea-predominant irritable bowel syndrome (IBS-D) patients show altered epithelial permeability and mucosal micro-inflammation in both proximal distal regions of the intestine. The objective this study was to determine molecular events mechanisms clinical role upper small intestinal alterations.Clinical assessment a jejunal biopsy obtained IBS-D healthy subjects. Routine histology immunohistochemistry performed all participants assess number mast cells (MCs) intraepithelial lymphocytes....
<h3>Objective</h3> Micro-RNAs (miRNAs) play a crucial role in controlling intestinal epithelial barrier function partly by modulating the expression of tight junction (TJ) proteins. We have previously shown differential messenger RNA (mRNA) correlated with ultrastructural abnormalities patients diarrhoea-predominant IBS (IBS-D). However, participation miRNAs these mRNA-associated findings remains to be established. Our aims were (1) identify differentially expressed small bowel mucosa IBS-D...
<h3>Background and aims</h3> Altered intestinal barrier is associated with immune activation clinical symptoms in diarrhoea-predominant IBS (IBS-D). Increased mucosal antigen load may induce specific responses; however, local antibody production its contribution to aetiopathogenesis remain undefined. This study evaluated the role of humoral activity IBS-D. <h3>Methods</h3> A single jejunal biopsy, luminal content blood were obtained from healthy volunteers (H; n=30) IBS-D (n=49; Rome III...
Summary Background Intestinal immune activation is involved in irritable bowel syndrome ( IBS ) pathophysiology. While most dietary approaches involve food avoidance, there are fewer indications on supplementation. Palmithoylethanolamide, structurally related to the endocannabinoid anandamide, and polydatin compounds which act synergistically reduce mast cell activation. Aim To assess effect count efficacy of palmithoylethanolamide/polydatin patients with . Methods We conducted a pilot,...
The interface between the intestinal lumen and mucosa is location where majority of ingested immunogenic particles face scrutiny vast gastrointestinal immune system. Upon regular physiological conditions, microflora epithelial barrier are well prepared to process daily a huge amount food-derived antigens non-immunogenic particles. Similarly, they ready prevent environmental toxins microbial penetrate further interact with mucosal-associated These functions promote development proper...
Abstract Background Intestinal epithelial dysfunction is a common pathophysiologic feature in irritable bowel syndrome (IBS) patients and might be the link to its clinical manifestations. We previously showed that chronic psychosocial stress induces jejunal barrier dysfunction; however, whether this response gender‐specific thus explain enhanced female susceptibility IBS remains unknown. Methods responses acute were compared age‐matched groups of healthy women men ( n = 10 each) experiencing...
Background and goal Diarrhoea‐predominant irritable bowel syndrome (IBS‐D) exhibits intestinal innate immune mucosal mast cell (MC) activation. MC stabilisers have been shown to improve IBS symptoms but the mechanism is unclear. Our primary aim was investigate effect of oral disodium cromoglycate (DSCG) on jejunal activation specific signalling pathways in IBS‐D, secondarily, its potential clinical benefit. Study Mucosal (by ultrastructural changes, tryptase release gene expression) protein...
Digestive disorders of gut-brain interaction (DGBI) are very common, predominant in females, and usually associated with intestinal barrier dysfunction, dysbiosis, stress. We previously found that females have increased susceptibility to dysfunction response acute However, whether this is changes the small bowel microbiota remains unknown. evaluated stress better understand stress-induced dysfunction. Jejunal biopsies were obtained at baseline 90 min after cold pain or sham Autonomic (blood...
Abstract Corticotropin-releasing factor (CRF) has been identified in intestinal mucosal eosinophils and associated with psychological stress gut dysfunction. Irritable bowel syndrome (IBS) is commonly characterized by altered motility, immune activation, increased barrier permeability along heightened susceptibility to psychosocial stress. Despite intensive research, the role of stress-associated dysfunction remains uncertain. In this study, we evaluated eosinophil activation profile CRF...
To determine the effect of peripheral CRF on intestinal barrier function in diarrhea-predominant IBS (IBS-D). Irritable bowel syndrome (IBS) pathophysiology has been linked to life stress, epithelial dysfunction, and mast cell activation. Corticotropin-releasing factor (CRF) is a major mediator stress responses gastrointestinal tract, yet its role mucosal remains largely unknown.Intestinal response sequential i.v. 5-mL saline solution (placebo) (100 μg) was evaluated 21 IBS-D 17 healthy...
Intestinal transit assessment in mice using existing methods requires long recording periods or euthanization of animals to localize a tracer. We have developed novel vivo method assess gastrointestinal (GI) based on clinically used 'shapes study'.Mice (n = 70) were gavaged with 5 steel beads and barium 3 h before, another dose 10 min before imaging. Mice fluoroscoped for 20-60 s, then most them euthanized the GI tract removed confirm localization fluoroscopically. The postmortem recordings...
Disturbed intestinal epithelial barrier and mucosal micro-inflammation characterize irritable bowel syndrome (IBS). Despite intensive research demonstrating ovarian hormones modulation of IBS severity, there is still limited knowledge on the mechanisms underlying female predominance in this disorder. Our aim was to identify molecular pathways involved dysfunction diarrhea-predominant (IBS-D) patients. Total RNA protein were obtained from jejunal biopsies healthy controls IBS-D patients...
Irritable bowel syndrome (IBS) is a disorder of brain-gut interaction characterised by abdominal pain and changes in habits. In the diarrhoea subtype (IBS-D), altered epithelial barrier mucosal immune activation are associated with clinical manifestations. We aimed to further evaluate plasma cells integrity gain understanding IBS-D pathophysiology. One jejunal biopsy one stool sample were obtained from healthy controls patients. Gastrointestinal symptoms, stress, depression scores recorded....
Liver injury has been widely described in patients with Coronavirus disease 2019 (COVID-19). We aimed to study the effect of liver biochemistry alterations, previous disease, and value elastography on hard clinical outcomes COVID-19 patients. conducted a single-center prospective observational 370 consecutive admitted for polymerase chain reaction (PCR)-confirmed pneumonia. Clinical laboratory data were collected at baseline parameters events recorded during follow-up. Transient [with...
Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder linked to intestinal barrier dysfunction and life stress. We have previously reported that female sex per se determines an increased susceptibility after cold pain stress (CPS). aimed identify sex-related molecular differences in response CPS healthy subjects understand the origin of bias predominance IBS. In 13 males 21 females, two consecutive jejunal biopsies were obtained using Watson’s capsule, at baseline, ninety...