A5048790165- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Gut microbiota and health
- Folate and B Vitamins Research
- Gene expression and cancer classification
- Pancreatic function and diabetes
- Alzheimer's disease research and treatments
- Malaria Research and Control
- Adipokines, Inflammation, and Metabolic Diseases
- Mosquito-borne diseases and control
- Gastrointestinal motility and disorders
- Statistical Methods and Inference
- Nutritional Studies and Diet
- Metabolism and Genetic Disorders
- Biosensors and Analytical Detection
- Dementia and Cognitive Impairment Research
- Veterinary Pharmacology and Anesthesia
- Muscle metabolism and nutrition
- Nutrition and Health in Aging
- Lipid metabolism and biosynthesis
Nestlé (Switzerland)
2015-2024
Nestlé (United Kingdom)
2024
École Polytechnique Fédérale de Lausanne
2014-2022
Anaxomics (Spain)
2019
University of Oxford
2010-2018
Nestlé (France)
2018
Comprehensive, high throughput analysis of the plasma proteome has potential to enable holistic health state an individual. Based on our own experience and evaluation recent large-scale mass spectrometry (MS) based proteomic studies, we identified two outstanding challenges: slow delicate nano-flow liquid chromatography (LC) irreproducibility identification data-dependent acquisition (DDA). We determined optimal solution reducing these limitations with robust capillary-flow data-independent...
The overall impact of proteomics on clinical research and its translation has lagged behind expectations. One recognized caveat is the limited size (subject numbers) (pre)clinical studies performed at discovery stage, findings which fail to be replicated in larger verification/validation trials. Compromised study designs insufficient statistical power are consequences to-date still capacity mass spectrometry (MS)-based workflows handle large numbers samples a realistic time frame, while...
Altered proteome profiles have been reported in both postmortem brain tissues and body fluids of subjects with Alzheimer disease (AD), but their broad relationships AD pathology, amyloid tau-related neurodegeneration not yet fully explored. Using a robust automated MS-based proteomic biomarker discovery workflow, we measured cerebrospinal fluid (CSF) proteomes to explore association well-established markers core pathology. Cross-sectional analysis was performed on CSF collected from 120...
Our recent randomized, placebo-controlled study in Irritable Bowel Syndrome (IBS) patients with diarrhea or alternating bowel habits showed that the probiotic Bifidobacterium longum (BL) NCC3001 improves depression scores and decreases brain emotional reactivity. However, involved metabolic pathways remain unclear. This analysis aimed to investigate biochemical underlying beneficial effects of BL using metabolomic profiling. Patients received (1x 1010CFU, n=16) placebo (n=19) daily for 6...
Abstract Holistic human proteome maps are expected to complement comprehensive profile assessment of health and disease phenotypes. However, methodologies analyze proteomes in tissue or body fluid samples at relevant scale performance still limited clinical research. Their deployment demonstration large enough populations even sparser. In the present study, we have characterized compared plasma two independent cohorts obese overweight individuals using shotgun mass spectrometry (MS)-based...
The systems-level relationship between the proteomes of cerebrospinal fluid (CSF) and plasma has not been comprehensively described so far. Recently developed shotgun proteomic workflows allow for deeper characterization from body fluids in much larger sample size. We deployed state-of-the-art mass spectrometry-based proteomics paired CSF samples volunteered by 120 elders with without cognitive impairment to characterize examine compartmental proteome differences relationships both fluids....
Introduction Sprint interval training (SIT) can elicit similar or even higher skeletal muscle mitochondrial adaptations than moderate-intensity continuous (MICT) despite a lower volume. We have previously shown that this is at least partially driven by improved bioenergetics with increased Ca2+ uptake and pyruvate dehydrogenase (PDH) activation after single session of SIT but not MICT [1]. Olive leaf extract (OLE) natural compound triggers activates PDH in mouse [2]. Here we tested the...
Over the past decade, mass spectrometric performance has greatly improved in terms of sensitivity, dynamic range, and speed. By contrast, only limited progress been accomplished with regard to automation, throughput, robustness proteomic sample preparation process upstream spectrometry. The present work delivers an optimized analysis human plasma samples both small preclinical large clinical studies, enabled by development a highly automated quantitative workflow. Several iterative...
Purpose The nutritional intervention program “DiOGenes” focuses on how obesity can be prevented and treated from a dietary perspective. We generated differential plasma proteome profiles in the DiOGenes cohort to identify proteins associated with weight loss maintenance explore their relation body mass index, fat mass, insulin resistance, sensitivity. Experimental design Relative protein quantification was obtained at baseline after combined loss/maintenance phases using isobaric tagging...
Over the last two decades, EDTA-plasma has been used as preferred sample matrix for human blood proteomic profiling. Serum also employed widely. Only a few studies have assessed difference and relevance of proteome profiles obtained from plasma samples, such or lithium-heparin-plasma, serum. A more complete evaluation use EDTA-plasma, heparin-plasma, serum would greatly expand comprehensiveness shotgun proteomics samples. In this study, we evaluated heparin-plasma with respect to profile...
Large-scale proteomic studies have to deal with unwanted variability, especially when samples originate from different centers and multiple analytical batches are needed. Such variability is typically added throughout all the steps of a clinical research study, human biological sample collection storage, preparation, spectral data acquisition, peptide protein quantification. In order remove such diverse normalization performed. There been already several published reviews comparing methods...
Growth failure and delayed puberty are well known features of children adolescents with inflammatory bowel disease (IBD), in addition to the chronic course disease. Urinary metabonomics was applied order better understand metabolic changes between healthy IBD children.21 Pediatric patients (mean age 14.8 years, 8 males) were enrolled from Gastroenterology Outpatient Clinic over two years. Clinical biological data collected at baseline, 6, 12 months. 27 12.9 16 assessed baseline. Urine...
Constitutional thinness (CT) is a state of low but stable body weight (BMI ≤18 kg/m2). CT subjects have normal-range hormonal profiles and food intake exhibit resistance to gain despite living in the modern world's obesogenic environment.The goal this study identify molecular mechanisms underlying protective phenotype against gain.We conducted clinical overfeeding on 30 controls 20-25 kg/m2) matched for age sex. We performed integrative transcriptomic analyses white adipose muscle...
Soft (fuzzy) clustering techniques are often used in the study of high-dimensional datasets, such as microarray and other high-throughput bioinformatics data. The most widely method is fuzzy C-means (FCM) algorithm, but it can present difficulties when dealing with some datasets. A DifFUZZY, which utilises concepts from diffusion processes graphs applicable to a larger class problems than algorithms developed. Examples datasets (synthetic real) for this outperforms frequently presented,...
Aim: There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover co-factors. Results: We report development validation a method simultaneous detection 13 red blood cells. The method, validated cohort healthy human volunteers, shows high level accuracy reproducibility. Conclusion: This high-throughput protocol provides robust coverage central co-factors one...
Background:Cerebrospinal fluid (CSF) biomarkers of the beta-amyloid and microtubule associated protein tau metabolism have proven capacity to improve classification subjects developing Alzheimer's disease (AD). The blood plasma proteome was characterized further elaborate upon mechani sms involved identify proteins that may older adults an AD dementia. Objective:Identify describe expressions best classify with CSF-defined presence pathology cerebral amyloidosis. Methods:We performed a...
Glucose is the main secretagogue of pancreatic beta-cells. Uptake and metabolism nutrient stimulates beta-cell to release blood glucose lowering hormone insulin. This metabolic activation associated with a pronounced increase in mitochondrial respiration. stimulation also initiates number signal transduction pathways for coordinated regulation multiple biological processes required insulin secretion. Shotgun proteomics including TiO2 enrichment phosphorylated peptides followed by liquid...
Mitochondria play a central role in pancreatic β-cell nutrient sensing by coupling their metabolism to plasma membrane excitability and insulin granule exocytosis. Whether non-nutrient secretagogues stimulate mitochondria as part of the molecular mechanism promote secretion is not known. Here, we show that PKC signaling, which employed many secretagogues, augments mitochondrial respiration INS-1E (rat insulinoma cell line clone 1E) human β cells. The phorbol ester, 12-myristate 13-acetate,...
While insulin resistance (IR) is associated with specific metabolite signatures in adults, there have been few truly longitudinal studies healthy children, either to confirm which abnormalities are present, or determine whether they precede result from IR. Therefore, we investigated the association of serum metabolites IR childhood Earlybird cohort.The cohort a well-characterized children annual measurements age 5 16 years. For first time, analyses between individual and homeostatic model...
The production of reactive oxygen species (ROS) may promote immunosenescence if not counterbalanced by the antioxidant systems. Cell membranes, proteins, and nucleic acids become target ROS progressively lose their structure functions. This process could lead to an impairment immune response. However, little is known about capability cells elderly individuals dynamically counteract oxidative stress. Here, response main lymphocyte subsets induced stress in semisupercentenarians (CENT),...