A5013201567- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Cardiac Ischemia and Reperfusion
- Sirtuins and Resveratrol in Medicine
- Ion channel regulation and function
- Electrochemical sensors and biosensors
- Trace Elements in Health
- 3D Printing in Biomedical Research
- Phytochemicals and Antioxidant Activities
- Calcium signaling and nucleotide metabolism
- Microfluidic and Capillary Electrophoresis Applications
- Cell death mechanisms and regulation
- Diet and metabolism studies
- Polyamine Metabolism and Applications
- Autophagy in Disease and Therapy
- Physical Activity and Health
- Redox biology and oxidative stress
- Molecular Communication and Nanonetworks
Nestlé (Switzerland)
2015-2025
École Polytechnique Fédérale de Lausanne
2012-2023
Nestlé (France)
2016
University of Geneva
2009-2014
University of Padua
2001-2010
Medium-chain triglycerides have been used as part of a ketogenic diet effective in reducing epileptic episodes. The health benefits the derived medium-chain fatty acids (MCFAs) are thought to result from stimulation liver ketogenesis providing fuel for brain. We tested whether MCFAs direct effects on energy metabolism induced pluripotent stem cell-derived human astrocytes and neurons. Using single-cell imaging, we observed an acute pronounced reduction mitochondrial electrical potential...
Mitochondria capture and subsequently release Ca(2+) ions, thereby sensing shaping cellular signals. The uniporter MCU mediates uptake, whereas NCLX (mitochondrial Na/Ca exchanger) LETM1 (leucine zipper-EF-hand-containing transmembrane protein 1) were proposed to exchange against Na(+) or H(+), respectively. Here we study the role of these ion exchangers in mitochondrial extrusion Ca(2+)-metabolic coupling. Both proteins expressed HeLa cells mitochondria. rate efflux, measured with a...
Plant polyphenols exhibit a variety of potentially useful biochemical properties in vitro, but their evaluation and clinical exploitation vivo is hampered by limited bioavailability. Precursors exhibiting resistance to phase II metabolism during absorption are therefore desirable. We report here the synthesis as well stability solubility studies several ester derivatives quercetin (3,3',4',5,7-pentahydroxy flavone), most which comprise an aminoacyl group. To model transepithelial absorption,...
Background and Purpose Quinic acid (QA) is an abundant natural compound from plant sources which may improve metabolic health. However, little attention has been paid to its effects on pancreatic beta‐cell functions, contribute the control of health by lowering blood glucose. Strategies targeting signal transduction are a new approach for diabetes treatment. This study investigated efficacy QA stimulate function basic molecular machinery metabolism–secretion coupling. Experimental Approach...
Mitochondrial dysfunction is linked to numerous pathological states, in particular related metabolism, brain health and ageing. Nuclear encoded gene polymorphisms implicated mitochondrial functions can be analyzed the context of classical genome wide association studies. By contrast, DNA (mtDNA) variants are more challenging identify analyze for several reasons. First, contrary diploid nuclear genome, each cell carries hundred copies circular genome. Mutations therefore present only a subset...
Mitochondria-targeted compounds are needed to act on a variety of processes that take place in these subcellular organelles and have great pathophysiological relevance. In particular, redox-active molecules capable homing mitochondria provide tool intervene major cellular source reactive oxygen species the they induce, notably mitochondrial permeability transition cell death. We linked 3-OH quercetin (3,3',4',5,7-pentahydroxy flavone), model polyphenol, triphenylphosphonium moiety,...
Pancreatic β-cells secrete insulin to lower blood glucose, following a meal. Maintenance of β-cell function is essential preventing type 2 diabetes. In pancreatic β-cells, mitochondrial matrix calcium an activating signal for secretion. Recently, the molecular identity uniporter (MCU), transporter that mediates uptake, was revealed. Its role in transduction modulation clarified, opening new perspectives intervention. Here, we investigated effects Ca2+-targeted nutritional intervention...
Abstract Sustained ryanodine receptor (RyR) Ca 2+ leak is associated with pathological conditions such as heart failure or skeletal muscle weakness. We report that a single session of sprint interval training (SIT), but not moderate intensity continuous (MICT), triggers RyR1 protein oxidation and nitrosylation leading to calstabin1 dissociation in healthy human vitro SIT models (simulated S-SIT). This accompanied by decreased sarcoplasmic reticulum content, increased levels mitochondrial...
The mitochondrial permeability transition pore and Bax have both been proposed to be involved in the release of pro-apoptotic factors from mitochondria "intrinsic" pathway apoptosis. is widely thought a supramolecular complex including or interacting with Bax. Given relevance vivo, we verified whether influences formation and/or properties Ca(2+)/P(i)-induced by using mitochondriaisolated isogenic human colon cancer bax(+/-) bax(-/-) HCT116 cell lines. We used isolated types cells Bax(+)...
In type 1 diabetes, a renewable source of human pancreatic β cells, in particular from induced pluripotent stem cell (hiPSC) origin, would greatly benefit therapy. Earlier work showed that progenitors differentiated embryonic cells vitro can further mature to become glucose responsive following macroencapsulation and transplantation mice. Here we took similar approach optimizing the generation hiPSCs. This demonstrates hiPSCs endoderm be efficiently robustly generated under large-scale...
Mitochondria isolated from engineered mice lacking Cyclophilin D (CypD), a component of the Permeability Transition Pore (PTP) complex, can still undergo Ca2 + -dependent but Cyclosporin A-insensitive permeabilization inner membrane. Higher concentrations are required than for wild-type controls. The characteristics pore formed in this system were not known, and it has been proposed that they might differ substantially those normal PTP. To test hypothesis, we have characterized PTP isogenic...
In pancreatic β-cells, mitochondria generate signals that promote insulin granule exocytosis. Here we study how lysine acetylation of mitochondrial proteins mechanistically affects metabolism-secretion coupling in insulin-secreting cells. Using mass spectrometry-based proteomics, identified sites rat insulinoma cell line clone 1E cells lacking the deacetylase sirtuin-3 (SIRT3), several matrix are hyperacetylated. Disruption SIRT3 gene has a deleterious effect on energy metabolism and Ca2+...
Regulation of mitochondrial redox balance is emerging as a key event for cell signaling in both physiological and pathological conditions. However, the link between state modulation these conditions remains poorly defined. Here, we discovered that activation evolutionary conserved calcium uniporter (MCU) modulates state. By using mitochondria-targeted sensors genetic MCU-ablated models, provide evidence causality MCU net reduction (but not cytosolic) Redox redox-sensitive groups via...