A5012262545- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Mass Spectrometry Techniques and Applications
- Diet, Metabolism, and Disease
- Fatty Acid Research and Health
- Consumer Attitudes and Food Labeling
- Gut microbiota and health
- Circadian rhythm and melatonin
- Diet and metabolism studies
- Phytoestrogen effects and research
- Microbial Metabolic Engineering and Bioproduction
- Meat and Animal Product Quality
- Nutritional Studies and Diet
- Muscle metabolism and nutrition
- Legume Nitrogen Fixing Symbiosis
- Analytical Chemistry and Chromatography
- Birth, Development, and Health
- Probiotics and Fermented Foods
- Nutrition and Health in Aging
- Metabolism and Genetic Disorders
- Porphyrin Metabolism and Disorders
- Nutrition, Genetics, and Disease
- Advanced Proteomics Techniques and Applications
- Dietary Effects on Health
- Dye analysis and toxicity
Nestlé (Switzerland)
2015-2025
École Polytechnique Fédérale de Lausanne
2016-2019
Blood plasma has gained protagonism in lipidomics studies due to its availability, uncomplicated collection and preparation, informative readout of physiological status. At the same time, it is also technically challenging analyze complex lipid composition affected by many factors, which can hamper throughput and/or coverage. To tackle these issues, we developed a comprehensive, high throughput, quantitative mass spectrometry-based shotgun platform for blood analyses. The main hallmarks this...
The microbiome has been demonstrated to play an integral role in the maintenance of many aspects health that are also associated with aging. In order identify areas potential exploration and intervention, we simultaneously characterized age-related alterations gut microbiome, muscle physiology serum proteomic lipidomic profiles aged rats define integrated signature aging phenotype. We demonstrate skews composition particular by altering Sutterella Barneseilla ratio, alters metabolic...
Significance While increasing evidence associates the disruption of circadian rhythms with pathologic conditions, including obesity, type 2 diabetes, and nonalcoholic fatty liver diseases (NAFLD), involved mechanisms are still poorly described. Here, we show that, in both humans mice, pathogenesis NAFLD is associated clock combined perturbations growth hormone sex pathways. However, while this condition protects mice from development fibrosis insulin resistance, it correlates increased...
Abstract Background Sunflower seeds ( Helianthus annuus ) display an attractive source for the rapidly increasing market of plant-based human nutrition. Of particular interest are press cakes seeds, cheap residuals from sunflower oil manufacturing that offer sustainability and economic benefits. Admittedly, seed milk, derived therefrom, suffers limited nutritional value, undesired flavor, presence indigestible sugars. specific relevance is absence vitamin B 12 . This required development...
We present a high-throughput, nontargeted lipidomics approach using liquid chromatography coupled to high-resolution mass spectrometry for quantitative analysis of nonesterified fatty acids. applied this method screen wide range acids from medium-chain very long-chain (8 24 carbon atoms) in human plasma samples. The enables us chromatographically separate branched-chain species their straight-chain isomers as well biologically important ω-3 and ω-6 polyunsaturated used 51 acid demonstrate...
Abstract The field of lipidomics has evolved vastly since its creation 15 years ago. Advancements in mass spectrometry have allowed for the identification hundreds intact lipids and lipid mediators. However, because release fatty acids from phospholipid membrane brain caused by ischemia, identifying neurolipidome been challenging. Microwave fixation shown to reduce ischemia‐induced several Therefore, this study aimed develop a method combining high‐resolution tandem ( MS / ), high‐energy...
The gut microbiome and lipid metabolism are both recognized as essential components in the maintenance of metabolic health. mechanisms involved multifactorial (especially for microbiome) poorly defined. A strategic approach to investigate complexity microbial influence on would facilitate determination relevant molecular microbiome-targeted therapeutics. E. coli is associated with obesity syndrome we used this association conjunction gnotobiotic models impact metabolism. To address...
Rationale Long‐chain fatty acyl‐coenzyme As (FA‐CoAs) are important bioactive molecules, playing key roles in biosynthesis of acids, membrane trafficking and signal transduction. Development sensitive analytical methods for profiling theses lipid species various tissues is critical to understand their biological activity. A high‐pressure liquid chromatography/tandem mass spectrometry method has been developed the quantitative analysis screening long‐chain FACoAs liver, brain, muscle adipose...
BackgroundMicronutrient supplementation has been extensively explored as a strategy to improve health and reduce risk of chronic diseases. Fat-soluble vitamins like A E with their antioxidant properties mechanistic interactions lipoproteins, have potentially key impact on lipid metabolism lipidemia.ObjectiveThe micronutrients requires further investigation including characterization plasma lipidome following any cause-effect circulating lipids.DesignIn this study, we elucidate the effect...
Abstract Through evolution, most of the living species have acquired a time keeping system to anticipate daily changes caused by rotation Earth. In all systems this pacemaker is based on molecular transcriptional/translational negative feedback loop able generate rhythmic gene expression with period close 24 hours. Recent evidences suggest that post-transcriptional regulations activated mostly systemic cues play fundamental role in process, fine tuning and linking it animal physiology. Among...
Abstract Background and objectives The presence of endocrine disruptors in food is creating concern, consequently, efficient methods for their identification are needed. In vitro bioassays have been developed to study properties pure chemicals as well mixtures. Because relative ease execution low cost, application such techniques whole foods tempting. This raises a number technical challenges. To address them, was commissioned contract laboratory offering estrogenicity testing products....
The idea that previously unknown hazards can be readily revealed in complex mixtures such as foods is a seductive one, giving rise to the hope data from effect-based assays of food products collected market surveys suitable quality basis for data-driven decision-making. To study this, we undertook comparative oestrogenicity blinded cereal samples, both number external testing laboratories and our own facility. results clearly showed little variance activities 9 samples when using single...