A5046371134- RNA modifications and cancer
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Genetic Associations and Epidemiology
- Genetics, Aging, and Longevity in Model Organisms
- Neurogenetic and Muscular Disorders Research
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Folate and B Vitamins Research
- Genomics and Rare Diseases
- Diet and metabolism studies
- Circadian rhythm and melatonin
- Education, sociology, and vocational training
- Dysphagia Assessment and Management
- Human Rights and Immigration
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Digestive system and related health
- Bacteriophages and microbial interactions
- Medication Adherence and Compliance
- Legume Nitrogen Fixing Symbiosis
University of Lausanne
2013-2025
Nestlé (Switzerland)
2015-2024
Nestlé (France)
2021
École Polytechnique Fédérale de Lausanne
2008-2020
Nestlé (United Kingdom)
2019
Nestlé (Brazil)
2019
Bibliothèque et Archives nationales du Québec
2018
University of Geneva
2013-2014
University of Bern
2007-2008
Centre National de la Recherche Scientifique
2006
The causes of impaired skeletal muscle mass and strength during aging are well-studied in healthy populations. Less is known on pathological age-related wasting weakness termed sarcopenia, which directly impacts physical autonomy survival. Here, we compare genome-wide transcriptional changes sarcopenia versus age-matched controls biopsies from 119 older men Singapore, Hertfordshire UK Jamaica. Individuals with reproducibly demonstrate a prominent signature mitochondrial bioenergetic...
Diurnal oscillations of gene expression are a hallmark rhythmic physiology across most living organisms. Such controlled by the interplay between circadian clock and feeding rhythms. Although mRNA accumulation has been extensively studied, comparatively less is known about their transcription translation. Here, we quantified simultaneously temporal transcription, accumulation, translation mouse liver mRNAs under physiological light-dark conditions ad libitum or night-restricted in WT brain...
Although adverse early life experiences have been found to increase lifetime risk develop violent behaviors, the neurobiological mechanisms underlying these long-term effects remain unclear. We present a novel animal model for pathological aggression induced by peripubertal exposure stress with face, construct and predictive validity. show that male rats submitted fear-induction during period exhibit high sustained rates of increased at adulthood, even against unthreatening individuals,...
Abstract Endosomal nucleic acid sensing by Toll-like receptors (TLRs) is central to antimicrobial immunity and several autoimmune conditions such as systemic lupus erythematosus (SLE). The innate immune adaptor TASL mediates, via the interaction with SLC15A4, activation of IRF5 downstream human TLR7, TLR8 TLR9, but pathophysiological functions this axis remain unexplored. Here we show that SLC15A4 deficiency results in a selective block TLR7/9-induced activation, while loss leads strong...
Endogenous retroviruses (ERVs) undergo de novo DNA methylation during the first few days of mammalian embryogenesis, although factors that control targeting this process are largely unknown. We asked whether KAP1 (KRAB-associated protein 1) is involved in mechanism because its previously defined role maintaining silencing ERVs through histone methyltransferase ESET and H3 lysine 9 trimethylation. Here, we demonstrate introduced ERV sequences sufficient to direct rapid a flanked promoter...
During hematopoiesis, lineage- and stage-specific transcription factors work in concert with chromatin modifiers to direct the differentiation of all blood cells. We explored role KRAB-containing zinc finger proteins (KRAB-ZFPs) their cofactor KAP1 this process. In mice, hematopoietic-restricted deletion Kap1 resulted severe hypoproliferative anemia. Kap1-deleted erythroblasts failed induce mitophagy-associated genes retained mitochondria. This was due persistent expression microRNAs...
Temporal control of physiology requires the interplay between gene networks involved in daily timekeeping and tissue function across different organs. How circadian clock interweaves with tissue-specific transcriptional programs is poorly understood. Here, we dissected temporal regulation at multiple regulatory layers by examining mouse tissues an intact or disrupted over time. Integrated analysis uncovered two distinct modes underlying rhythms: oscillations transcription factor (TF)...
Rationale: Human milk oligosaccharides (HMOs) vary among mothers and genetic factors contribute to this variability. We assessed changes in HMO concentrations during the first year of lactation relationship with FUT2 Secretor group FUT3 Lewis defining polymorphisms. Methods: Milk samples were collected from lactating participating LIFE Child cohort Leipzig, Germany. The 24 HMOs at 3 months (N = 156), 6 122), 12 28) measured using liquid chromatography. Concentrations compared all time-points...
Extreme longevity is the paradigm of healthy aging as individuals who reached extreme decades human life avoided or largely postponed all major age-related diseases. In this study, we sequenced at high coverage (90X) whole genome 81 semi-supercentenarians and supercentenarians [105+/110+] (mean age: 106.6 ± 1.6) 36 unrelated geographically matched controls age 68.0 5.9) recruited in Italy. The results showed that 105+/110+ are characterized by a peculiar genetic background associated with...
Our recent randomized, placebo-controlled study in Irritable Bowel Syndrome (IBS) patients with diarrhea or alternating bowel habits showed that the probiotic Bifidobacterium longum (BL) NCC3001 improves depression scores and decreases brain emotional reactivity. However, involved metabolic pathways remain unclear. This analysis aimed to investigate biochemical underlying beneficial effects of BL using metabolomic profiling. Patients received (1x 1010CFU, n=16) placebo (n=19) daily for 6...
Drug resistance threatens the effective control of infections, including parasitic diseases such as leishmaniases.Neutrophils are essential players in antimicrobial control, but their role drug-resistant infections is poorly understood.Here, we evaluated human neutrophil response to clinical parasite strains having distinct natural drug susceptibility.We found that Leishmania antimony significantly altered expression genes, some them transcribed by specific subsets.Infection with parasites...
CUG-BP1 [CUG-binding protein 1 also called CELF (CUG-BP1 and ETR3 like factors) 1] is a human RNA-binding that has been implicated in the control of splicing mRNA translation. The Xenopus homologue [EDEN-BP (embryo deadenylation element-binding protein)] required for rapid certain maternal mRNAs just after fertilization. A variety sequence elements have described as target sites these two proteins but their binding specificity still controversial. Using SELEX (systematic evolution ligand by...
In spinal muscular atrophy (SMA), the leading genetic cause of early childhood death, survival motor neuron 1 gene ( SMN1 ) is deleted or inactivated. The nearly identical SMN2 has a silent mutation that impairs utilization exon 7 and production functional protein. It been hypothesized therapies boosting inclusion might prevent cure SMA. Exon can be stimulated in cell culture by oligonucleotides intracellularly expressed RNAs, but evidence for an vivo improvement SMA symptoms lacking. Here,...
Hyperhomocysteinemia is a risk factor for cognitive decline and dementia, including Alzheimer disease (AD). Homocysteine (Hcy) sulfur-containing amino acid metabolite of the methionine pathway. The interrelated methionine, purine, thymidylate cycles constitute one-carbon metabolism that plays critical role in synthesis DNA, neurotransmitters, phospholipids, myelin. In this study, we tested hypothesis metabolites beyond Hcy are relevant to function cerebrospinal fluid (CSF) measures AD...
Mitochondrial dysfunction is linked to numerous pathological states, in particular related metabolism, brain health and ageing. Nuclear encoded gene polymorphisms implicated mitochondrial functions can be analyzed the context of classical genome wide association studies. By contrast, DNA (mtDNA) variants are more challenging identify analyze for several reasons. First, contrary diploid nuclear genome, each cell carries hundred copies circular genome. Mutations therefore present only a subset...
Objective: The ApoE (apolipoprotein) allele epsilon 4 is a major genetic risk factor for Alzheimer disease, cardiovascular disorders, and stroke, indicating that it significantly impacts cerebral vascular systems. However, very little known about how APOE genotype affects brain endothelial cells, which form network of tight junctions to regulate communication between the circulating blood factors. Approach Results: Here, we present novel model dysfunction using isogenic human induced...
Abstract Environmental pseudomonads colonize various niches including insect and plant environments. When invading these environments, bacteria are confronted with the resident microbiota. To oppose closely related strains, they rely on narrow-spectrum weaponry such as tailocins, i.e., phage tail-like particles. Little is known about receptors for tailocins especially among phylogenetically species. Here, we studied interaction between an R-tailocin from Pseudomonas protegens CHA0 a targeted...
While methylated cytosines are known to play important roles in eukaryotes, their significance bacteria remains poorly understood especially when they added on genomes by DNA methyltransferases that not parts of restriction-modification systems. The newly named ScmA protein Caulobacter crescentus is one these solitary cytosine methyltransferases. Here, we show it methylates YGCCGGCR motifs introducing thousands m5C bases the C. genome. We also find wild-type cells easily outcompete ΔscmA...
Spinal muscular atrophy (SMA) is a lethal hereditary disease caused by homozygous deletion/inactivation of the survival motoneuron 1 (SMN1) gene. The nearby SMN2 gene, despite its identical coding capacity, only an incomplete substitute, because single nucleotide difference impairs inclusion seventh exon in messenger RNA (mRNA). This splicing defect can be corrected (transiently) specially designed oligonucleotides. Here we have developed more permanent correction strategy based on...
Translation depends on messenger RNA (mRNA)-specific initiation, elongation, and termination rates. While translation elongation is well studied in bacteria yeast, less known higher eukaryotes. Here we combined ribosome transfer (tRNA) profiling to investigate the relations between rates, (aminoacyl-) tRNA levels, codon usage mammals. We modeled codon-specific dwell times from profiling, considering pair interactions sites. In mouse liver, model revealed site- that differed those as pairs of...
Abstract Background The cline of human genetic diversity observable across Europe is recapitulated at a micro-geographic scale by variation within the Italian population. Besides resulting from extensive gene flow, this might be ascribable also to local adaptations diverse ecological contexts evolved people who anciently spread along Peninsula. Dissecting evolutionary history ancestors present-day Italians may thus improve understanding demographic and biological processes that contributed...