A5000853147- Epigenetics and DNA Methylation
- Pain Mechanisms and Treatments
- Neurological diseases and metabolism
- Dietary Effects on Health
- Family Support in Illness
- Botulinum Toxin and Related Neurological Disorders
- Pain Management and Opioid Use
- Identity, Memory, and Therapy
- Immune responses and vaccinations
- Genomics and Rare Diseases
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Genetics and Neurodevelopmental Disorders
- Bone health and osteoporosis research
- HIV/AIDS Research and Interventions
- Nuclear Receptors and Signaling
- Telomeres, Telomerase, and Senescence
- GDF15 and Related Biomarkers
- Gene expression and cancer classification
- Biological Research and Disease Studies
- interferon and immune responses
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Genetics, Bioinformatics, and Biomedical Research
- Artificial Intelligence in Healthcare
University of Bologna
2020-2025
March of Dimes
2021
Extreme longevity is the paradigm of healthy aging as individuals who reached extreme decades human life avoided or largely postponed all major age-related diseases. In this study, we sequenced at high coverage (90X) whole genome 81 semi-supercentenarians and supercentenarians [105+/110+] (mean age: 106.6 ± 1.6) 36 unrelated geographically matched controls age 68.0 5.9) recruited in Italy. The results showed that 105+/110+ are characterized by a peculiar genetic background associated with...
The aim of the present study was to provide a comprehensive characterization whole genome DNA methylation patterns in replicative and ionizing irradiation- or doxorubicin-induced premature senescence, exhaustively exploring epigenetic modifications three different human cell types: somatic diploid skin fibroblasts bone marrow- adipose-derived mesenchymal stem cells. With CpG-wise differential analysis, signatures were identified: (a) type- treatment-specific signature; (b) type-specific...
Perilipin 2 (PLIN2) is a lipid droplet (LD)-coating protein playing important roles in homeostasis and suppression of lipotoxicity different tissues cell types. Recently, role for PLIN2 supporting mitochondrial function has emerged. dysregulation involved many metabolic disorders age-related diseases. However, the exact consequences are not yet completely understood. In this study, we knocked down (KD) primary human dermal fibroblasts (hDFs) from young (mean age 29 years) old 71 healthy...
Epigenetic clocks were initially developed to track chronological age, but accumulating evidence indicates that they can also predict biological age. They are usually based on the analysis of DNA methylation by genome-wide methods, targeted approaches, assessment a small number CpG sites, advisable in several settings. In this study, we epigenetic clock purposely optimized for measurement The includes six genomic regions mapping ELOVL2, NHLRC1, AIM2, EDARADD, SIRT7 and TFAP2E genes, selected...
BACKGROUND. Predicting Immune-effector Cell Associated Neurotoxicity Syndrome (ICANS) in patients infused with Chimeric Antigen Receptor T cells (CAR-T) is still a conundrum. This complication, thought to be consequent CAR-T cell activation, arises few days after infusion, when circulating are scarce and specific cell-derived biomarkers lacking.
Abstract Background Individuals with type 2 diabetes (T2D) face an increased mortality risk, not fully captured by canonical risk factors. Biological age estimation through DNA methylation (DNAm), i.e . the epigenetic clocks, is emerging as a possible tool to improve stratification for multiple outcomes. However, whether these tools predict independently of factors in subjects T2D unknown. Methods Among cohort 568 patients followed 16.8 years, we selected subgroup 50 subjects, 27 survived...
<p dir="ltr">The aim of this work was to describe the DNA methylation signature and identify genes associated with neuropathic pain in type 2 diabetes mellitus.</p><p dir="ltr">We analyzed two independent diabetic neuropathy cohorts: PROPGER consisting 72 painful 67 painless patients recruited at German Diabetes Center Düsseldorf (DE), PROPENG comprising 27 65 University Manchester (UK). Genome-wide data generated using Illumina Infinium Methylation EPIC v1.0 BeadChip. We...
<p dir="ltr">The aim of this work was to describe the DNA methylation signature and identify genes associated with neuropathic pain in type 2 diabetes mellitus.</p><p dir="ltr">We analyzed two independent diabetic neuropathy cohorts: PROPGER consisting 72 painful 67 painless patients recruited at German Diabetes Center Düsseldorf (DE), PROPENG comprising 27 65 University Manchester (UK). Genome-wide data generated using Illumina Infinium Methylation EPIC v1.0 BeadChip. We...
The aim of this work was to describe the DNA methylation signature and identify genes associated with neuropathic pain in type 2 diabetes mellitus. We analyzed two independent diabetic neuropathy cohorts: PROPGER consisting 72 painful 67 painless patients recruited at German Diabetes Center Düsseldorf (DE), PROPENG comprising 27 65 University Manchester (UK). Genome-wide data generated using Illumina Infinium Methylation EPIC v1.0 BeadChip. used four different selection criteria promising...
Abstract About one out of two diabetic patients develop neuropathy (DN), these 20% experience neuropathic pain (NP) leading to individual, social, and health-economic burden. Risk factors for NP are largely unknown; however, premature aging was recently associated with several chronic disorders. DNA methylation-based biological age (DNAm) is disease risk, morbidity, mortality in different clinical settings. The purpose this work study, the first time, whether involved development a huge...
Abstract Recent literature shows that loss of replicative ability and acquisition a proinflammatory secretory phenotype in senescent cells is coupled with the build-in nucleic acids cytoplasm. Its implication human age-related diseases under scrutiny. In endothelial (ECs), we assessed accumulation intracellular during vitro senescence after exposure to high glucose concentrations, which mimic an vivo condition hyperglycemia. We showed induces senescent-like features ECs, including telomere...
Subcutaneous adipocytes are crucial for mammary gland epithelial development during pregnancy. Our and others’ previous data have suggested that adipo-epithelial transdifferentiation could play a key role in the alveolar development. In this study, we tested whether occurs vitro. Data show that, under appropriate co-culture conditions with organoids (MEOs), mature lose their phenotype acquire an one. Interestingly, even absence of MEOs, extracellular matrix diffusible growth factors able to...
Healthcare workers (HCWs) are a high-risk group for hepatitis B virus (HBV) infection. Notably, about 5–10% of the general population does not respond to HBV vaccination. In this study, we aimed investigate DNA methylation (DNAm) in order estimate biological age cells from HCW both sexes, either responder (R) or non-responder (NR), We used genome-wide data calculate set biomarkers collected 41 Rs and 30 NRs between 22 62 years old. Unresponsiveness vaccination was associated with accelerated...
Advanced age is the major risk factor for idiopathic Parkinson's disease (PD), but to date biological relationship between PD and ageing remains elusive. Here we describe rationale design of H2020 funded project "PROPAG-AGEING", whose aim characterize contribution process development. We summarize current evidences that support existence a continuum justify use Geroscience approach study PD. focus in particular on role inflammaging, chronic, low-grade inflammation characteristic elderly...
Chronic pain prevalence is high worldwide and increases at older ages. Signs of premature aging have been associated with chronic pain, but few studies investigated biomarkers in pain-related conditions. A set DNA methylation (DNAm)-based estimates age, called "epigenetic clocks", proposed as biological measures age-related adverse processes, morbidity mortality. The aim this study was to determine if different phenotypes show alterations DNAm age. We considered our analysis 3 cohorts for...
Abstract Objective Atypical femur fractures (AFFs) are rare fragility originating at the lateral cortex of femur, affecting subtrochanteric or diaphyseal area thebone with a transverse morphology. Occurrence AFF is specifically associated small number monogenic congenital metabolic bone disorders, such as hypophosphatasia, and long-term treatment antiresorptiondrugs. The exact pathogenesis these remains poorly understood and, except for cases diagnosed HPP other AFF-causing diseases, it not...
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Background/Objectives: HBV infections can lead to serious liver complications that have fatal consequences. In 2022, around 1.1 million individuals died from HBV-related cirrhosis and hepatocellular carcinoma. Vaccines allow us save more than 2.5 lives each year; however, up 10% of vaccinated may not develop sufficient protective antibody levels. The aim this study was investigate the epigenetic drift in response vaccine isolated B cells. Methods: Epigenetic measured by counting rare DNA...
Abstract Alzheimer’s disease (AD) is characterized by specific alterations of brain DNA methylation (DNAm) patterns. Age and sex, two major risk factors for AD, are also known to largely affect the epigenetic profiles in brain, but their contribution AD-associated DNAm changes has been poorly investigated. In this study we considered publicly available datasets 4 regions (temporal, frontal, entorhinal cortex cerebellum) from healthy adult subjects AD patients, performed a meta-analysis...