A5017850039- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Mycorrhizal Fungi and Plant Interactions
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Fungal Biology and Applications
- Circular RNAs in diseases
- RNA Research and Splicing
- Coenzyme Q10 studies and effects
- Exercise and Physiological Responses
- Molecular Biology Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Autophagy in Disease and Therapy
- Receptor Mechanisms and Signaling
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Plant Pathogens and Fungal Diseases
- Plant nutrient uptake and metabolism
- Sports Performance and Training
- Caveolin-1 and cellular processes
- Fungal and yeast genetics research
- Cell Adhesion Molecules Research
- Adipokines, Inflammation, and Metabolic Diseases
University of Urbino
2015-2024
Marche Polytechnic University
2017
Istituti di Ricovero e Cura a Carattere Scientifico
2012
In the past few years, skeletal muscle has emerged as an important secretory organ producing soluble factors, called myokines, that exert either autocrine, paracrine or endocrine effects. Moreover, recent studies have shown releases microRNAs into bloodstream in response to physical exercise. These affect target cells, such hormones and cytokines. The mechanisms underlying microRNA secretion are poorly characterized at present. Here, we investigated whether tissue extracellular vesicles...
The role of epigenetics in endothelial cell senescence is a cutting-edge topic ageing research. However, little known the relative contribution to pro-senescence signal propagation provided by microRNAs shuttled extracellular vesicles (EVs) released from senescent cells. Analysis microRNA and DNA methylation profiles non-senescent (control) (SEN) human umbilical vein cells (HUVECs), profiling their cognate small EVs (sEVs) large demonstrated that SEN significantly greater sEV number than...
Real-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnostics. Although, the cycle-threshold (Ct) method present "gold standard", it far from being standard assay. Uniform reaction efficiency among samples most important assumption of this method. Nevertheless, some authors have reported may not be correct slight decrease about 4% could result an error up to 400% using Ct This caused...
Despite the development of combined modality treatments against liposarcoma in recent years, a significant proportion patients respond only modestly to such approaches, possibly contributing local or distant recurrence. Early detection recurrent metastatic disease could improve patient prognosis by triggering earlier clinical intervention. However, useful biomarkers for purposes are lacking. Using both plasma samples and cell lines, we demonstrate here that miR-25-3p miR-92a-3p secreted...
Abstract It is known that the MyoD family members (MyoD, Myf5, myogenin, and MRF4) play a pivotal role in complex mechanism of skeletal muscle cell differentiation. However, fragmentary information on transcription factor‐specific regulation available data their post‐transcriptional post‐translational behavior are still missing. In this work, we combined mRNA protein expression analysis with subcellular localization. Each myogenic regulator factor (MRF) revealed specific trend quantitative...
Extrasynaptic neurotransmission is an important short distance form of volume transmission (VT) and describes the extracellular diffusion transmitters modulators after synaptic spillover or extrasynaptic release in local circuit regions binding to activating mainly neuronal glial receptors neuroglial networks brain. Receptor-receptor interactions G protein-coupled receptor (GPCR) heteromers play a major role, on dendritic spines nerve terminals including glutamate synapses, integrative...
Type 2 diabetes (T2D) is an age-related chronic disease associated with metabolic dysregulation, inflammation, and activation of peripheral blood mononuclear cells (PBMC). The aim this study was to assess the effects a concurrent exercise training program on inflammatory status parameters T2D patients. Sixteen male patients (age range 55-70) were randomly assigned intervention group (n = 8), which underwent aerobic resistance (3 times week; 16 weeks), or control group, followed physicians'...
Abstract The therapeutic ability of Mesenchymal Stem/Stromal Cells to address osteoarthritis (OA) is mainly related the secretion biologically active factors, which can be found within their secreted Extracellular Vesicles including small (sEV). Aim this study was investigate effects sEV from adipose derived stromal cells (ADSC) on both chondrocytes and synoviocytes, in order gain insights into mechanisms modulating inflammatory/catabolic OA environment. sEV, obtained by a combined...
Abstract Creatine (Cr), one of the most popular nutritional supplements among athletes, has been recently shown to prevent cytotoxicity caused by different oxidative stressors in various mammalian cell lines, including C2C12 myoblasts, via a direct antioxidant activity. Here, effect Cr on differentiating capacity cells exposed H 2 O investigated. Differentiation into myotubes was monitored using morphological, ultrastructural, and molecular techniques. Treatment with (1 h) not only...
Abstract Results are presented that were obtained on the geographic traceability of white truffle Tuber magnatum Pico. Solid‐phase microextraction coupled to gas chromatography/mass spectrometry (SPME‐GC/MS) was employed characterize volatile profile T . produced in seven geographical areas Italy. The main components fraction identified using SPME‐GC/MS. Significant differences proportion constituents from truffles different detected. results suggest that, besides genetic factors,...
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mass membrane potential analysed Mitotracker Green JC-1 stains epifluorescence microscope. Expression of PGC1α, NRF1α, Tfam genes controlling biogenesis was studied real-time PCR. The...
Myogenic differentiation is triggered, among other situations, in response to muscle damage for regenerative purposes. It has been shown that during myogenic differentiation, myotubes release extracellular vesicles (EVs) which participate the signalling pattern of microenvironment. Here we investigated whether EVs released by exposed or not mild oxidative stress modulate behaviour targeted differentiating myoblasts and macrophages promote myogenesis. We found oxidatively challenged...
Growing evidence points to the effectiveness of flywheel (FW) based iso-inertial resistance training in improving physical performance capacities. However, molecular adaptations induced by FW exercises are largely unknown. Eight resistance-trained men performed 5 sets 10 maximal squats on a device. Muscle biopsies (fine needle aspiration technique) and blood samples were collected before (t0), 2h (t1) after exercise. Blood additionally drawn 24h (t2) 48h (t3). Paired t-tests revealed...
Extracellular vesicles (EVs) play a crucial role in the intercellular crosstalk. Mesenchymal stem cell-derived EVs (MSC-EVs), displaying promising therapeutic roles, contribute to strong rationale for developing as an alternative option. EV analysis still represents one of major issues be solved order translate use MSC-EV detection clinical settings. Even if flow cytometry (FC) has been largely applied studies, lack consensus on protocols FC generated controversy. Standard procedures, based...
Plant-derived nanovesicles (PDNVs) have become attractive alternatives to mammalian cell-derived extracellular vesicles (EVs) both as therapeutic approaches and drug-delivery vehicles. In this study, we isolated tomato fruit-derived NVs separated them by the iodixanol density gradient ultracentrifugation (DGUC) into twelve fractions. Three visible bands were observed at densities 1.064 ± 0.007 g/mL, 1.103 0.006 g/mL 1.122 0.012 g/mL. Crude PDNVs DGUC fractions characterized particle...
Exercise-released extracellular vesicles (EVs) are emerging as a novel class of exerkines that promotes systemic beneficial effects. However, slight differences in the applied exercise protocols terms mode, intensity and duration, well need for standardized EV isolation, make comparison studies literature extremely difficult. This work aims to investigate amount EV-associated miRNAs released circulation response different physical regimens. Healthy individuals were subjected protocols: acute...