A5026786600- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Electrospun Nanofibers in Biomedical Applications
- Oral microbiology and periodontitis research
- Autophagy in Disease and Therapy
- Laser Applications in Dentistry and Medicine
- Bone and Dental Protein Studies
- Wound Healing and Treatments
- Dental Implant Techniques and Outcomes
- Immune Response and Inflammation
- Graphene and Nanomaterials Applications
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Neonatal Respiratory Health Research
- RNA Interference and Gene Delivery
- Cannabis and Cannabinoid Research
- Oral and gingival health research
- Inflammasome and immune disorders
- Vitamin C and Antioxidants Research
- Endodontics and Root Canal Treatments
- 3D Printing in Biomedical Research
University of Chieti-Pescara
2016-2025
Manchester Metropolitan University
2016
The role of bone tissue engineering in the field regenerative medicine has been a main research topic over past few years. There much interest use three-dimensional (3D) engineered scaffolds (PLA) complexed with human gingival mesenchymal stem cells (hGMSCs) as new therapeutic strategy to improve regeneration. These devices can mimic more favorable endogenous microenvironment for vivo by providing 3D substrates which are able support cell survival, proliferation and differentiation. present...
Bone regeneration represents still a challenge, in particular for calvarium defects. Recently, the development of biomaterials with addiction stem cells is giving promising results treatment bone In particular, it was demonstrated that scaffolds enriched mesenchymal (MSCs) and/or their derivatives, such as conditioned medium (CM) and extracellular vesicles (EVs), may improve regeneration. Moreover, given deep link between osteogenesis angiogenesis, successful approach must also take into...
Abstract Background Multiple sclerosis is a demyelinating disease mostly of autoimmune origin that affects and damages the central nervous system, leading to disabling condition. The aim present study was investigate whether administration mesenchymal stem cells from human periodontal ligament (hPDLSCs) could ameliorate multiple progression by exerting neuroprotective effects in an experimental model encephalomyelitis (EAE). Methods EAE induced immunization with myelin oligodendroglial...
Abstract Manipulation of stem cells or cells-derived secretome has emerged as a novel alternative therapeutic option for multiple sclerosis (MS). Here we show that human periodontal ligament (hPDLSCs)-derived conditioned medium (hPDLSCs-CM) and purified exosomes/microvesicles (hPDLSCs-EMVs) obtained from Relapsing Remitting (RR)-MS patients healthy donors block experimental autoimmune encephalomyelitis (EAE), mouse model MS, by inducing anti-inflammatory immunosuppressive effects in spinal...
Mesenchymal stem cells (MSCs) have emerged as a promising tool for the treatment of several neurodegenerative disorders, including Alzheimer’s disease (AD). The main neuropathological hallmarks AD are senile plaques, composed amyloid beta (Aβ), and neurofibrillary tangles, formed by hyperphosphorylated tau. However, current therapies shown limited efficacy. In this study, we evaluated whether pre-treatment with cannabidiol (CBD), at 5 μM concentration, modulated transcriptional profile MSCs...
Unresolved inflammation and tissue destruction are underlying mechanisms of periodontitis, which is linked to dysregulated polymorphonuclear neutrophil (PMN) functions. Lipoxin A4 (LXA4) a specialized proresolving lipid mediator (SPM) that dampens excessive inflammation, promotes resolution, protects from leukocyte-mediated damage. Human periodontal ligament stem cells (hPDLSCs) represent key players during regeneration may contribute resolution inflammation; thus, they promising tool in...
Curcumin, primary component of the spice turmeric extracted from rhizomes Curcuma longa, represents major anti-oxidant and anti-inflammatory substance found in turmeric, acting thought various mechanisms not completely understood. Curcumin modulates cytokines, growth factors, transcription inflammatory molecules cell signaling pathways. During restorative dentistry practice, free resin monomers 2-hydroxyethyl methacrylate (HEMA) propagate through dentin micro-channel pulp into bloodstream...
Bone tissue regeneration strategies require approaches that provide an osteogenic and angiogenic microenvironment able to drive the bone growth. Recently, development of 3D printing biomaterials, including poly(lactide) (3D-PLA), enriched with mesenchymal stem cells (MSCs) and/or their derivatives, such as extracellular vesicles (EVs) has been achieving promising results. In this study, in vitro results showed increased expression markers, RUNX2, VEGFA, OPN COL1A1 living construct...
Purpose: The combination of oral derived stem cells and 3-D scaffolds is considered advantageous in bone repair. In particular, collagen membranes possess ideal biological properties can support infiltration proliferation osteoblasts, promoting regeneration. Our study aimed to develop a new biocompatible osteogenic construct composed commercially available membrane (Evolution [Evo]), human periodontal-ligament (hPDLSCs) enriched with extracellular vesicles (EVs), or polyethylenimine...
<p>The present study was aimed at investigating whether human Periodontal Ligament Stem Cells (hPDLSCs) were capable of sensing and reacting to lipopolysaccharide from <em>Porphyromonas</em> <em>gingivalis</em> (LPS-G) which is widely recognized as a major pathogen in the development progression periodontitis. At this purpose hPDLCs stimulated with 5 μg/mL LPS-G various times expression toll-like receptor 4 (TLR4) evaluated. Toll-like receptors (TLRs) play an...
Bone tissue engineering is one of the main branches regenerative medicine. In this field, use a scaffold, which supported bone development, in combination with mesenchymal stem cells (MSCs), has promised better outcomes for regeneration. particular, human gingival (hGMSCs) may present advantages compared to other MSCs, including easier isolation. However, MSCs’ secretome attracted much attention its potential regeneration, such as conditioned medium (CM) that contains different soluble...
Recent research has widely investigated the anti-inflammatory effects of mesenchymal stem cells and their secretory products, termed secretome, in treatment multiple sclerosis (MS). The present study examined capacity conditioned medium (CM) from human periodontal ligament (hPLSCs) under hypoxia (H-hPDLSCs-CM) to suppress experimental autoimmune encephalomyelitis (EAE), a murine model MS. To induce EAE, female C57BL/6 mice were immunized with myelin oligodendroglial glycoprotein peptide35-55...
Background . Neural crest-derived mesenchymal stem cells (MSCs) from human oral tissues possess immunomodulatory and regenerative properties are emerging as a potential therapeutic tool to treat diverse diseases, such multiple sclerosis, myocardial infarction, connective tissue damages. In addition cell-surface antigens, dental MSCs express embryonic cell markers neural crest originate the ectoderm layer. vitro passages may eventually modify these marker expressions other stemness...
Bone tissue engineering is based on bone grafting to repair defects. graft substitutes can contribute the addition of mesenchymal stem cells (MSCs) in order enhance rate and quality defect regeneration. The cell secretome contains many growth factors chemokines, which could affect cellular characteristics behavior. Conditioned medium (CM) be used regeneration avoiding several problems linked direct use MSCs. In this study, we investigated effect human periodontal ligament (hPDLSCs) their CM...
Research in recent years has largely explored the immunomodulatory effects of mesenchymal stem cells (MSCs) and their secretory products, called "secretome," treatment neuroinflammatory diseases. Here, we examined whether such immunosuppressive might be elicited due to inflammasome inactivation. To this end, treated experimental autoimmune encephalomyelitis (EAE) mice model multiple sclerosis (MS) with conditioned medium or purified exosomes/microvesicles (EMVs) obtained from...
Human Gingival Mesenchymal Stem Cells (hGMSC) are multipotential cells that can expand and differentiate in culture under specific standardized conditions. In the present study, we have investigated whether vitro pre-treatment of hGMCs with Cannabidiol (CBD) influence their expression profile, improving therapeutic potential this cell culture. Following CBD treatment (5μM) for 24 h, gene analysis through Next Generation Sequencing (NGS) has revealed several genes differentially expressed...
Spinal cord injury (SCI) is a neurological disorder that arises from primary acute mechanical lesion, followed by pathophysiological cascade of events leads to further spinal tissue damage. Several preclinical and clinical studies have highlighted the ability stem cell therapy improve long-term functional recovery in SCI. Previously, we demonstrated moringin (MOR) treatment accelerates differentiation process mesenchymal cells inducing an early up-regulation neural development associated...