The antioxidant activity of flavonoids is believed to increase when they are coordinated with transition metal ions. However, the literature on this subject contradictory and outcome seems largely depend experimental conditions. In order understand contribution coordination type interaction between a flavonoid ion, in study new complex Cu (II) naringin was synthesized characterized by FT-IR, UV-VIS, mass spectrometry (ESI-MS/MS), elemental analysis 1H-NMR. results these analyses indicate...

Abstract Background It has been speculated that the biostimulatory effect of Low Level Laser Therapy could cause undesirable enhancement tumor growth in neoplastic diseases. The aim present study is to analyze behavior melanoma cells (B16F10) vitro and vivo development mice after laser irradiation. Methods We performed a controlled on B16F10 investigate cell viability cycle changes by Tripan Blue, MTT quest histogram tests at 24, 48 72 h post mouse model (male Balb C, n = 21) was used volume...

This study aimed to evaluate the systemic inflammatory response and cardiovascular changes induced by experimental periodontitis in rats. Experimental was placing a cotton ligature around cervix of both sides mandibular first molars maxillary second each male rat. Sham-operated rats had removed immediately after procedure. Seven, 14 or 28 days procedure, effects acetylcholine, sodium nitroprusside phenylephrine were evaluated on blood pressure, aortic rings isolated perfused mesenteric bed....

Brazilian descendants of former Black-slave (quilombola) communities have been predisposed to several zoonotic diseases due social vulnerability, characterized by subsistence and close contact with livestock companion animals. Accordingly, the present study has assessed anti-Coxiella burnetii antibodies in 200 individuals 20 dogs from four quilombola located Paraná State, southern Brazil. Serum samples were tested indirect immunofluorescence assay (IFA) using in-house commercial diagnostic...

O objetivo do presente estudo é analisar o perfil sociodemográfico e a produção técnico-científica dos bolsistas de produtividade Conselho Nacional Desenvolvimento Científico Tecnológico, na área Educação Física, no período 2017 2020. Para tal, obteve-se lista pesquisadores da Física com bolsa através uma consulta à página eletrônica CNPq aos seus respectivos currículos lattes Plataforma Lattes CNPq. A contabilização dados registrados nos todos os ocorreu auxílio software scriptLattes v8.10....

Abstract This study aimed to assess the efficacy of a new formulation containing melatonin precursors in patients with sleep disorders. Thirty-six individuals were divided into two groups: one receiving and other placebo, for 30 days. Sleep quality was evaluated using Pittsburgh Quality Index (PSQI) questionnaire before after intervention. It showed significant improvements quality, including reduced time fall asleep, fewer nighttime awakenings, higher overall satisfaction, group...

Although quilombola individuals and their dogs may be exposed to hemoparasites such as A. phagocytophilum, B. microti, E. chaffeensis, no study date has been conducted in these populations. The aim of this was investigate the presence antibodies against Anaplasma Babesia Ehrlichia chaffeensis humans from communities Brazil. Serum samples were collected four rural analyzed using indirect immunofluorescence assays. results revealed antibody levels 8% for 3% 1% 60%, 50%, 65%, respectively,...

Protein energy malnutrition (PEM) is a syndrome that often results in immunodeficiency coupled with pancytopenia. Hemopoietic tissue requires high nutrient supply and the proliferation, differentiation maturation of cells occur constant balanced manner, sensitive to demands specific cell lineages dependent on stem population. In present study, we evaluated effect PEM some aspects hemopoiesis, analyzing cycle bone marrow percentage progenitor marrow. Two-month-old male Swiss mice (N = 7-9 per...

Impaired cell metabolism and increased death in fibroblast cells are physiological features of chronic tendinopathy. Although several studies have shown that low-level laser therapy (LLLT) at certain parameters has a biostimulatory effect on cells, it remains uncertain if LLLT effects depend the state.High-metabolic immortal culture primary human keloid were used this study. Trypan blue exclusion 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test to determine viability...

Brazilian quilombos are rural semi-isolated remnant communities of former black slaves and their descendants who traditionally maintained themselves through archaic subsistence livestock agriculture practices historically lacked specific public health policies. Although such individuals dogs may be exposed to zoonotic pathogens as Toxoplasma gondii, no study date has assessed these human-animal populations together. Populations in four different southern Brazil were evaluated. Overall,...

Apocynin (APO), curcumin (CUR) and vanillin (VAN) are o-methyl catechols widely studied due their antioxidant antitumour properties. The effect of treatment with these on tamoxifen (TAM)-induced cytotoxicity in normal tumour cells was studied. TAM red blood (RBC) performed by haemoglobin or K(+)release polymorphonuclear leukocytes (PMNs) trypan blue dye exclusion method. Cytotoxic activity assessed human chronic myeloid leukemia (K562) cell line...

Statins induces cell cycle arrest, apoptosis, reduction of angiogenic factors, inhibition the endothelial growth factor, impairing tissue adhesion and attenuation resistance mechanisms. The aim this study was evaluate anti-tumoral activity simvastatin in a B16F10 melanoma-mouse model. Melanoma cells were treated with different concentrations assessed by viability methods. (5 × 104) implanted two month old C57Bl6/J mice. Around 7 days after injection, oral treatments started mg/kg/day, p.o.)....

Oxysterols are cholesterol oxygenated derivatives which possess several biological actions. Among oxysterols, 7-ketocholesterol (7KC) is known to induce cell death. Here, we hypothesized that 7KC cytotoxicity could be applied in cancer therapeutics. was incorporated into a lipid core nanoemulsion. As cellular model the murine melanoma line B16F10 used. The nanoparticle (7KCLDE) uptake tumor cells displaced by increasing amounts of low-density-lipoproteins (LDL) suggesting...

The gut has shown to have a pivotal role on the pathophysiology of metabolic disease. Food stimulation distal intestinal segments promotes enterohormones secretion influencing insulin metabolism. In diabetic rats, oral potential change epithelium behavior. This macromolecule positive effects laboratorial parameters and decreases hypertrophy. study aims test if can influence weight in obese non-diabetic rats.Twelve weeks old Wistar rats were divided 3 groups: control (CTRL) standard chow...

Curcumin is a natural compound presenting important antitumour activity. However, due to its low aqueous solubility, instability at physiological pH, and oral bioavailability, clinical use limited. Bovine serum albumin (BSA) nanoparticles have been used as drug carriers improve the properties. In this work, curcumin-loaded BSA were developed in vitro cytotoxicity over murine melanoma cells vivo activity model assessed. Nanoparticles presented 150 nm, polydispersity index of 0.16, negative...

AIM:To investigate the effects of bariatric surgery on metabolic parameters, incretin hormone secretion, and duodenal ileal mucosal gene expression. METHODS:Nine patients with type 2 diabetes mellitus (T2DM), chronic serum hyperglycemia for more than years, a body mass index (BMI) 30-35 kg/m underwent sleeve gastrectomy transit bipartition between May 2011 December 2011.Blood samples were collected pre 3, 6 12 mo postsurgery.Duodenal mucosa pre-and 3 postsurgery.Pre-and postoperative blood...

Abstract Amniotic fluid is a complex mixture composed of water, salts and different cells types derived from embryo exfoliation. Some these present similar characteristics to mesenchymal stem as adherent properties, typical surface antigens differentiation capacity. These are called amniotic fluid‐derived ( AFMSC s) easily obtained by amniocentesis, propagated in culture differentiated several cell with specific inductions. In this study, we observe the ability simvastatin, 3‐ HMG ‐CoA...

Background and Objective It has been demonstrated that periodontitis induces systemic inflammation, which may impair endothelial function leading to increased cardiovascular risk. The aim of this study was evaluate the effect simvastatin on inflammatory markers dysfunction induced by periodontitis. Material Methods Wistar rats were subjected ligature‐induced experimental Eight days after procedure, ligature sham groups randomly assigned receive or vehicle once a day until 14th day, when...