A5017996864- Vector-Borne Animal Diseases
- Vector-borne infectious diseases
- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Herpesvirus Infections and Treatments
- Identification and Quantification in Food
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Probiotics and Fermented Foods
- Milk Quality and Mastitis in Dairy Cows
- Poxvirus research and outbreaks
- Animal Disease Management and Epidemiology
- Rabies epidemiology and control
- T-cell and Retrovirus Studies
- Food Safety and Hygiene
- Burkholderia infections and melioidosis
- Advanced Chemical Sensor Technologies
- Bacillus and Francisella bacterial research
- Escherichia coli research studies
- Insect and Pesticide Research
- Shoulder and Clavicle Injuries
- Veterinary Orthopedics and Neurology
- Reproductive tract infections research
- Helminth infection and control
- Infectious Disease Case Reports and Treatments
Universidade Estadual Paulista (Unesp)
2014-2024
Universidade de São Paulo
2021
Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia
2015
The origin of Vaccinia virus (VACV) outbreaks in Brazil remains unknown, but since the isolation VACV Mus musculus mice during a zoonotic outbreak affecting cattle and milkers, peridomestic rodents have been suggested to be link between cows wild animals. Considering that experimentally infected eliminate viral particles their feces, we investigated presence feces urine were captured forest areas surrounding milking farms central west region São Paulo State. For first time, this work reports...
Brazilian descendants of former Black-slave (quilombola) communities have been predisposed to several zoonotic diseases due social vulnerability, characterized by subsistence and close contact with livestock companion animals. Accordingly, the present study has assessed anti-Coxiella burnetii antibodies in 200 individuals 20 dogs from four quilombola located Paraná State, southern Brazil. Serum samples were tested indirect immunofluorescence assay (IFA) using in-house commercial diagnostic...
Foodborne diseases represent a major risk to public health worldwide. Pathogenic bacteria can live in the form of biofilm within food industry, providing permanent source contamination. The aim this study was evaluate influence types adhesion surfaces on Salmonella formation at eight different times, and analyze action time bacteriophage pool established biofilms. Most samples used were classified as weak producers, with serovars Enteritidis Heidelberg showing highest frequency formation....
This work demonstrates the application of an electronic nose (e-nose) for discrimination between authentic and adulterated honey. The developed e-nose is based on electrodes covered with ionogel (ionic liquid + gelatin Fe3O4 nanoparticle) films. Authentic honey samples were submitted to analysis, capacity sensors was evaluated using principal component analysis (PCA) average relative response data. From PCA biplot, it possible note two well-defined clusters no intersection observed. To...
Q fever and brucellosis are zoonoses that cause other systemic clinical signs in humans; their occurrences neglected the differential diagnosis for some diseases is disregarded. This study aimed to investigate seropositivity Coxiella burnetii Brucella spp. antibodies patients suspected of dengue from 38 municipalities state São Paulo, Brazil. The samples (n = 604) were obtained by convenience Adolfo Lutz Institute serum bank. Sera subjected an indirect immunofluorescence assay (IFA) using...
The performance of a commercial immunofluorescence assay (IFA commercial), an in-house in-house) and indirect enzyme-linked immunosorbent (ELISA) were evaluated in the detection antibodies anti-C. burnetii serum Q fever patients persons without disease. For study, seropositive seronegative samples for (n = 200) from bank Instituto Adolfo Lutz Brazil used. Commercial IFA was considered this study as gold standard diagnosing fever. demonstrated good agreement with test, showing high...
Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution. Despite vast information about circulating genotypes in Europe and North America, there is lack data regarding C. burnetii strains South America. Here, we show presence novel multispacer sequence typing (MST) two clusters detected Brazil Argentina that seem to be distant parenthood. Argentinian isolated from tick belongs new phylogenetic branch Brazilians may related MST 20 61. Multilocus variable number tandem...
Q fever is an important zoonosis, yet it often neglected and can present large outbreaks, as observed in the Netherlands. In past few years, cases of have been described Brazil; however, epidemiological situation ruminants, main reservoir pathogen, unknown this country. Our study aimed to estimate prevalence C. burnetii cattle sent slaughterhouses using immunofluorescence assay (IFA) quantitative real-time PCR (qPCR). From 1515 serum samples collected from nine slaughterhouses, 23.8%...
During a vaccinia virus (VACV) outbreak in São Paulo State, Brazil, blood samples were collected from cows, humans, other domestic animals, and wild mammals. Samples 3 dogs opossums positive for VACV by PCR. Results of gene sequencing yielded major questions regarding mammalian species acting as reservoirs VACV.
Abstract Coxiella burnetii is a zoonotic pathogen with worldwide distribution that responsible for Q fever in humans. It highly infectious bacterium can be transmitted from cattle to humans through the consumption of unpasteurized milk. We report molecular identification C. raw cow's milk being sold directly human Brazil without official inspection or pasteurization. One hundred and twelve samples were analysed by real‐time quantitative PCR (qPCR), was detected 3.57% (4/112) at concentration...
Outbreaks of Vaccinia virus (VACV) affecting cattle and humans have been reported in Brazil the last 15 years, but origin outbreaks remains unknown. Although VACV DNA already detected mice (Mus musculus), opossums (Didelphis albiventris) dogs during zoonotic outbreaks, no transmission to or from any these were Brazilian outbreaks. In this work, we assessed PCR positivity blood samples cows other domestic mammals, wild rodents areas with without infection reports. Our results show detection...
ABSTRACT: Bovine mastitis has a negative impact on milk production and can pose risks to public health. The present study aimed evaluate the quality of bovine from small farms in Botucatu/SP region. Somatic cell counts (SCC), identification pathogens involved mastitis, sensitivity antimicrobial profile staphylococci isolated were performed. presence enterotoxin encoding genes isolates obtained was investigated. Milk samples individual mammary quarters cows submitted California test (CMT)...
Three culture media (Brucella agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination isolating Brucella spp. One hundred lymph nodes from pigs (n = 50) wild boars with lymphadenitis collected slaughterhouses the State of São Paulo assessed on these three selective All samples negative media. On agar fungal (70 plates) Gram-positive bacterial (59 contaminants observed; CITA absence bacteria 15 plates was no or growth observed The results demonstrated...
A brucelose é uma importante zoonose causada por bactérias do gênero Brucella. O homem infectado pelo contato com as secreções reprodutivas como placenta, lóquios placentários, sêmen e penianas de animais infectados ou consumo leite derivados não pasteurizados. Com o objetivo pesquisar a presença da bactéria no leite, foram avaliadas, através técnica reação em cadeia polimerase (PCR), 30 amostras cru comercializadas clandestinamente na região Botucatu, São Paulo, bem 50 entregues laticínio,...
Canine brucellosis, due to Brucella canis, is a worldwide zoonosis that remains endemic in South America, including Brazil. Implementation of powerful whole-genome sequencing approaches allowed exploring the genus considered as monomorphic, with, date, more than 500 genomes available public databases. Nevertheless, with under-representation B. canis -only twenty complete or draft genomes-, lack knowledge about this species still considerable. This report describes comparative genomics-based...
We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit environment dairy farms.We collected 217 insect specimens from nine farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing search VP1 N genes for bovine coronavirus-BCoV, respectively. With a predominance Muscidae (152/217 = 70%) 11 families Diptera identified. Rotavirus A (RVA) betacoronavirus (BCoV) detected 14.7% (32/217) 4.6% (10/217) dipterans, Sequencing...