Salmonella enterica serovar Enteritidis-lysing bacteriophages isolated from poultry or human sewage sources were used to reduce Enteritidis in vitro and experimentally infected chicks. Cocktails of 4 different obtained commercial broiler houses (CB4Ø) 45 a municipal wastewater treatment plant (WT45Ø) evaluated. In experiment 1, an crop assay was conducted with selected bacteriophage concentrations (105 109 pfu/mL) determine ability the simulated environment. Following 2 h at 37°C, CB4Ø WT45Ø...

Foodborne diseases represent a major risk to public health worldwide. Pathogenic bacteria can live in the form of biofilm within food industry, providing permanent source contamination. The aim this study was evaluate influence types adhesion surfaces on Salmonella formation at eight different times, and analyze action time bacteriophage pool established biofilms. Most samples used were classified as weak producers, with serovars Enteritidis Heidelberg showing highest frequency formation....

The effect of a commercial organic acid (OA) product on BW loss (BWL) during feed withdrawal and transportation, carcass yield, meat quality was evaluated in broiler chickens. Two experiments were conducted Brazil. Commercial houses paired as control groups receiving regular water treated OA the water. Treated birds had reduction BWL 37 g experiment 1 32.2 2. In 2, no differences observed yield between groups. Estimation cost benefit suggested 1:16 ratio by using OA. 3, Mexico, significant...

The expression of immune response in the form leukocytic infiltrate by CD3+, CD4+, and CD8+ cells epithelium intestinal lamina propria chicks was studied present work means immunohistochemical reaction. were treated with Lactobacillus spp. or cecal microflora (CM) experimentally challenged not Salmonella enterica serovar Enteritidis. 320 birds utilized divided into 4 groups containing 80 each submitted to treatments reuteri, salivarius, acidophilus, CM. Each group subdivided subgroups 20...

The protection level against Salmonella Enteritidis was evaluated in chickens after ovo treatment with different species of Lactobacillus spp. inoculated into the air cell or by immersion broth culture. Two hundred forty embryonated eggs were distributed 8 groups, corresponding to treatments acidophilus, fermentum, salivarius, and control. On d 18 incubation, 4 groups 0.1 mL inoculum immersed for 3 min culture each treatment. days hatching, 0.5 intraesophageal route. 5 life, chicks...

Because of recent interest in bacteriophage therapy poultry, information regarding the interaction bacteriophages and potential host bacteria environment should be collected. The present studies were initiated with a rather typical commercial broiler integrator within south-central United States to examine environmental Salmonella levels two complexes, attempt isolate Salmonella-lytic bacteriophages, elucidate possible reason for differing apparent prevalence. Significantly (P < 0.05) less...

This study aimed to characterize the in vitro and vivo adhesion immunomodulatory effect of Lactobacillus strains isolated from chickens. samples 65-wk-old birds were identified by PCR; their was evaluated via basement membrane-type cell matrix through carboxyfluorescein succinimidyl amino ester staining inoculation 1-d-old duodenum, jejunum, ileum, cecum collections at 1, 4, 12, 24 h after inoculation. The 5 best adhesive test formed a pool for total IgA IgG measurement sera intestinal...

Salmonella food poisoning is a public health problem. Feed withdrawal from broiler chickens before slaughter can favor the multiplication of in cecum and crop contaminated animals subsequently lead to contamination carcasses processing plant. In present study, cocktail lytic bacteriophages isolated sewage water was orally administered 45-d-old 1 h after they received an oral dose 10(7) cfu/mL enterica subspecies serotype Enteritidis. Immediately phage administration 30 min, 1, 3, 6, 12...

Necrotic Enteritis (NE) caused by Clostridium perfringens (CP) in poultry is probably the most important bacterial disease terms of economic implications. The multi-factorial and invariably associated with predisposing factors. present study investigated effect a commercially available Lactobacillus-based probiotic (FM-B11) for control necrotic enteritis broiler chickens. In experiment 1, one-day-of-hatch chicks were randomly allocated to following treatment groups: 1) Non-challenged (NC);...

A study was conducted to investigate the effects of organic acids (OA) and competitive exclusion product (CE) on growth performance, intestinal morphology, concentration volatile fatty in cecal content. The experiment lasted for 10 wk. Four hundred twenty 1-d-old female commercial cross turkey poults (British United Turkeys, BUT Big 9) were distributed into 4 treatments with 5 replicates/pen 21 birds each. fed a basal diet without promoter (control), lincomycin (44 mg/kg), (2 g/kg), CE...

Efficiency in poultry production is based on the balance between nutrition, intestinal health, and animal welfare. With increasing consumer demand for antibiotic suppression of feed growth, nutrition segment has sought new alternatives to optimize rates and, this concept, use probiotics—living microorganisms (beneficial bacteria yeast) health. The objective experiment was evaluate welfare broilers fed different doses probiotics. A randomized block experimental design with 4 treatments 10...

Avian salmonellosis is a disease caused by bacteria of the genus Salmonella that can cause three distinct diseases in birds: pullorum diseases, fowl typhoid, and paratyphoid infection. Various wildlife species are susceptible to infections Salmonella, regardless whether they live captivity or freely wild. The present study verified presence enterica serovar Enteritidis captive specimens Amazona aestiva. involved total 103 birds undergoing rehabilitation prepare for living wild, after having...

To evaluate the use of organic acids (OAs) and competitive exclusion (CE) product administered continuously in feed transiently drinking water on control Salmonella enterica subspecie serotype Enteritidis (SE) prior to slaughter.The influence treatments were evaluated pH, population lactic acid bacteria (LAB) family Enterobacteriaceae, concentration volatile fatty SE colonization crop caecum. The birds challenged with 24 h before being slaughtered, then, caeca removed subjected counts....

A Escherichia coli é uma bactéria pertencente à família Enterobacteriacea que faz parte da microbiota entérica de mamíferos e muitas espécies aves. Salmonella spp. também pertence mesma família, sendo responsável por surtos infecção alimentar em humanos frequentemente isoladas aves domésticas silvestres. O presente estudo analisou a frequência ambas as bactérias Psittaciformes processo reabilitação para vida livre. De 89 analisadas 19% estavam infectadas com E. 1,12% Realizou-se análise do...

In commercial layer poultry farming, molt induction is an important tool used by egg producers to prolong the production cycle of laying hens. Conventional programs involve total feed withdrawal, which raises questions about animal welfare and increased infection susceptibility. The high incidence paratyphoid salmonellosis infections in farming still health challenge because addition affecting birds, such also cause public problems. this context, experiments were performed with hens at 79 wk...

The effects of treatment with anaerobic cecal microflora (ACM) and/or lactose acetic acid on systemic and digestive tract broiler chicks infection Salmonella Typhimurium S. Enteritidis were studied. ACM was used without previous bacterial identification. Treatment contributed to the resistance spp. infections more persistent in cecum, rectum crops decreasing order intensity. also self-limiting since treated control lots presented similar rates at end experiments. Alone or combination...

Among the various sanitary problems affecting birds, parasitic diseases are some of most common ones. This study analyses prevalence endoparasites in 20 fecal samples wild and exotic birds attended at Ornitopathology Parasitic Diseases Laboratories Veterinary Hospital, Faculty Medicine Zootecnics, Sao Paulo State University (FMVZ-UNESP), campus Botucatu. After confirmation eggs, cysts or oocysts through direct test, Faust technique Willis Method were performed on feces. Eggs three genera...

The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. utilization polymerase chain reaction technique (PCR) can reduce this time, but substances present may affect reaction. work aimed to compare DNA extraction thermic treatment and use cetyltrimethil ammonium bromide (CTAB), products originated from poultry houses corresponding raw material (meat meal)...

The health of poultry is closely related to how the animals are raised, and gut microbiota plays a key role in fulfillment their productive potential. Lactobacillus spp. bacteria present natural that, when employed as probiotic agents, should several characteristics. This study aims at performing vitro isolation characterization Isolates were obtained from ceca content healthy turkeys through identification morphological, molecular, physiologic They identified Gram staining, catalase...

Lactobacilos foram isolados do inglúvio e cecos de reprodutoras pesadas caracterizados como Gram-positivo, catalase negativo, produtores gás em glicose não H2S triple sugar iron pela fermentação carboidratos. Utilizaram-se os iniciadores: Lac 1/23-10C para detecção Lactobacillus acidophilus, L. crispatus, amylovorus, gasseri, helveticus jensenii; 2/LU-1' acidophilus; Fer 3/Fer 4 fermentum; Reu 1/Reu 2 reuteri Sal 1 salivarius. salivarius identificados reação cadeia polimerase (PCR) pelo...

Inglúvios e cecos de reprodutoras comerciais frangos corte foram utilizados para o isolamento Lactobacillus spp. As estirpes isoladas caracterizadas como Gram-positivo, catalase negativo, produtoras gás em glicose, não H2S triple sugar iron identificadas pela reação cadeia da polimerase reuteri salivarius. A utilização técnica spot-on-the-lawn avaliação inibição in vitro permitiu a análise vários microrganismos simultaneamente. Todas as inibiram S. Enteritidis fagotipo 4, 28, Typhimurium,...