A5101850586- Mesenchymal stem cell research
- Laser Applications in Dentistry and Medicine
- Multiple Myeloma Research and Treatments
- Cancer Treatment and Pharmacology
- Cell Adhesion Molecules Research
- Boron Compounds in Chemistry
- RNA Interference and Gene Delivery
- Bioactive Natural Diterpenoids Research
- Synthesis and biological activity
- HIV/AIDS drug development and treatment
- Cancer Mechanisms and Therapy
- Tissue Engineering and Regenerative Medicine
- Synthesis and Characterization of Heterocyclic Compounds
- Chemokine receptors and signaling
- Cancer, Lipids, and Metabolism
- Protein Degradation and Inhibitors
- Immune cells in cancer
- Boron and Carbon Nanomaterials Research
- Cell death mechanisms and regulation
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Photodynamic Therapy Research Studies
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
Instituto Butantan
2015-2025
Universidade de São Paulo
1991-2024
Université Paris Cité
2001
Universidade Brasil
1991
Isolation of mesenchymal stem cells (MSCs) in equines, has been reported for different tissues including bone marrow, adipose, umbilical cord, peripheral blood, and yolk sac. In regard to the MSCs derived from synovial fluid (SF) or membrane (SM), there is data available humans, dogs, pigs, goats horses. Especially these have being considered promising candidates articular regeneration. Herein, we established characterized obtained equine SF SM. Samples were during arthroscopy cultured using...
Abstract Neutrophil homeostasis was investigated in two mouse lines, AIRmax and AIRmin, genetically selected for high or low acute inflammatory response (AIR) compared with unselected BALB/c mice. Mature neutrophil phenotype functions appeared similar the three lines. However, an unprecedented revealed animals characterized by a production bone marrow (BM), number of neutrophils blood, concentration chemotactic agents acrylamide-induced exudates, increased resistance locally infiltrated to...
Impaired cell metabolism and increased death in fibroblast cells are physiological features of chronic tendinopathy. Although several studies have shown that low-level laser therapy (LLLT) at certain parameters has a biostimulatory effect on cells, it remains uncertain if LLLT effects depend the state.High-metabolic immortal culture primary human keloid were used this study. Trypan blue exclusion 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test to determine viability...
The objective of this study was to evaluate the effect laser irradiation on dog bone marrow stem cells.Low doses low-level red positively affect viability mesenchymal cells, and also increase proliferation.Low-level (wavelength, 660 nm; power output, 50 mW), applied cell cultures (DBMSC). energy densities delivered varied from 1 12J/cm(2). evaluated proliferation measured with MTT colorimetric test, cycle phase, lipidic peroxidation (free radical production).The results indicate that DBMSC...
Abstract Background Bone marrow and adipose tissues are known sources of mesenchymal stem cells (MSCs) in horses; however, synovial might be a promising alternative. The aim this study was to evaluate phenotypic characteristics differentiation potential equine MSCs from fluid (SF) membrane (SM) healthy joints (SF-H SM-H), with osteoarthritis (SF-OA SM-OA) osteochondritis dissecans (SF-OCD SM-OCD) determine the most suitable source for an allogeneic therapy cell bank. Methods Expression...
Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, this work, fibroblasts tumor cells are submitted with doses of 2.5 Gy 10 Gy. After twenty-four-h, exposed fluences 30 J cm-2 , 90 150 . Cellular viability, cell cycle phases, proliferation index senescence evaluated on days...
Malignant melanoma is a less common but highly dangerous form of skin cancer; it starts in the melanocytes cells found outer layer skin. Jararhagin toxin, metalloproteinase isolated from Bothrops jararaca snake venom acts upon several biological processes, as inflammation, pain, platelet aggregation, proliferation and apoptosis, though not yet approved for use, may one day be employed to treat tumors. B16F10 murine were treated with jararhagin (jara), disintegrin-like venom, jari (catalytic...
Acute renal failure (ARF) is a common disease that can lead to high mortality. Recovery from ARF occurs with the replacement of necrotic tubular cells by functional epithelial and normalization microvascular endothelial cell function in peritubular capillaries. Conventional therapeutic techniques are often ineffective against ARF. Hence, stem therapies, which act through multiple trophic regenerative mechanisms, encouraging. We investigated homing human immature dental pulp (IDPSCs) after...
Stem cells constitute a group of great capacity for self-renewal, long-term viability, and multi-lineage potential. Several studies have provided evidence that adipose tissue represents an alternative source stem cells, with the main benefit adipose-derived being they can be easily harvested from patients by simple minimally invasive method cultured. The aim this study was to establish culture protocol obtaining characterizing (ADSCs) C57BL/6 J mice. results showed yield, cell morphology...
Abstract The new outbreak of coronavirus disease 2019 (COVID-19) has infected and caused the death millions people worldwide. Intensive efforts are underway around world to establish effective treatments. Immunoglobulin from immunized animals or plasma convalescent patients might constitute a specific treatment guarantee neutralization virus in early stages infection, especially with risk factors high probability progressing severe disease. Worldwide, few clinical trials using...
The bone morphogenetic proteins (BMPs) belong to a unique group of that includes the growth factor TGF-β. BMPs play important roles in cell differentiation, proliferation, and inhibition growth. They also participate maturation several types, depending on microenvironment interactions with other regulatory factors. Depending their concentration gradient, can attract various types cells act as chemotactic, mitogenic, or differentiation agents. interfere proliferation formation cartilage bone....
Cationic peptide with the sequence INKKI 41-45 was isolated from bovine β-casein after tryptic hydrolysis and synthetized. The aim of this work to evaluate antiproliferative activity in vitro antitumor effect animal model. cytotoxicity evaluated on B16F10 melanoma cells by MTT assay. Detection apoptosis measured using annexin V/PI double staining cell cycle analysis performed flow cytometry. Caspase-3 analyzed substrate specific fluorogenic DEVD-MCA. In vivo, tumor-bearing C57BL/6J mice....
Abstract Objectives To test the toxicity and antitumoral activity of compound N-oleyl-daunorubicin (oDNR) with a cholesterol-rich nanoemulsion (LDE) formulation. Methods LDE-oDNR was prepared by high-pressure homogenisation lipid mixtures. B16F10 melanoma cells NIH/3T3 fibroblasts were used for cytotoxicity tests. The maximum tolerated dose (MTD) both commercial determined in mice, melanoma-bearing mice Key findings CC50 DNR 200 μm 15 μm, respectively, but less toxic against than DNR. MTD...
Mice selected for a high acute inflammatory response (AIRmax) are resistant to chemically induced lung tumorigenesis, whereas the low responders (AIRmin) susceptible. In urethane-treated mice, anti-inflammatory drugs increased tumor incidence in AIRmax but not AIRmin and an inverse correlation (P<.001) between degree of (AIR) tumorigenesis was found F2 (AIRmax×AIRmin) intercross population. The results provide evidence involvement modifier loci AIR regulation implicate quantitative trait...
The role of innate immunity in natural resistance to tumor progression was investigated two mouse lines, AIRmax and AIRmin, selected by bi-directional selective breeding on the basis high or low acute inflammatory response. Compared with mice were shown be resistant 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate-induced skin cancers here we demonstrate that are also able restrain development metastases upon transfer MHC compatible, incompatible xenogeneic...