A5078017924- Cannabis and Cannabinoid Research
- GABA and Rice Research
- Natural product bioactivities and synthesis
- Biochemical Analysis and Sensing Techniques
- Essential Oils and Antimicrobial Activity
- Autophagy in Disease and Therapy
- Plant biochemistry and biosynthesis
- Neuroscience and Neuropharmacology Research
- Phytochemicals and Antioxidant Activities
- Olfactory and Sensory Function Studies
- Diet, Metabolism, and Disease
- Phytochemistry and Biological Activities
- Advanced Chemical Sensor Technologies
- Bioactive natural compounds
- Pancreatic function and diabetes
- Toxin Mechanisms and Immunotoxins
- Microbial Natural Products and Biosynthesis
- Natural Compound Pharmacology Studies
- Fungal Biology and Applications
- Cancer, Lipids, and Metabolism
- Sesquiterpenes and Asteraceae Studies
- Alkaloids: synthesis and pharmacology
- Peroxisome Proliferator-Activated Receptors
- Tryptophan and brain disorders
- Genomics, phytochemicals, and oxidative stress
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2016-2025
Piedmont University
2013-2020
Istituto di Farmacologia Traslazionale
2018
Probiotical (Italy)
2012-2016
One key to animal survival is the detection and avoidance of potentially harmful compounds by their bitter taste. Variable numbers taste 2 receptor genes expressed in gustatory end organs enable bony vertebrates (Euteleostomi) recognize numerous chemicals. It believed that receptive ranges repertoires match profiles chemicals species encounter diets. Human mouse genomes contain pairs orthologous have been conserved throughout evolution. Moreover, expansions both lineages generated...
Speed trap: Cysteamine reacts in DMSO with electrophilic double bonds to afford Michael adducts (see scheme), whereas no trapping reaction takes place apolar solvents. A simple and quick NMR spectroscopic method identify acceptors sort them into reversible irreversible thiol sinks is validated biologically a cellular assay sensitive thiol-trapping agents. DMSO=dimethyl sulfoxide. Detailed facts of importance specialist readers are published as "Supporting Information". Such documents...
Summary Parthenolide, a major constituent of feverfew, acts as partial agonist TRPA1. Parthenolide’s ability to target TRPA1 could explain its therapeutic effects on migraine. Although feverfew has been used for centuries treat pain and headaches is recommended migraine treatment, the mechanism protective action remains unknown. Migraine triggered by calcitonin gene-related peptide (CGRP) release from trigeminal neurons. Peptidergic sensory neurons express series transient receptor potential...
Mesenchymal stem cells (MSCs) have emerged as a promising tool for the treatment of several neurodegenerative disorders, including Alzheimer’s disease (AD). The main neuropathological hallmarks AD are senile plaques, composed amyloid beta (Aβ), and neurofibrillary tangles, formed by hyperphosphorylated tau. However, current therapies shown limited efficacy. In this study, we evaluated whether pre-treatment with cannabidiol (CBD), at 5 μM concentration, modulated transcriptional profile MSCs...
Inflammation and oxidative stress play main roles in neurodegeneration. Interestingly, different natural compounds may be able to exert neuroprotective actions against inflammation stress, protecting from neuronal cell loss. Among these sources, Cannabis sativa represents a reservoir of exerting beneficial properties, including cannabigerol (CBG), whose antioxidant properties have already been demonstrated macrophages. Here, we aimed evaluate the ability CBG protect NSC-34 motor neurons...
Five compounds representative of major structural classes lichen polyketides, viz. (+)-usnic (1), salazinic (2), vulpinic (3), gyrophoric (4), and evernic acids (5), were investigated for their ability to affect cell proliferation or wound healing, two functional targets relevance research on cancer tissue regeneration. The experiments carried out MM98 malignant mesothelioma cells, A431 vulvar carcinoma HaCaT keratinocytes. NRU CV cytotoxicity assays showed high toxicity acid, intermediate...
Abstract Scleroderma is a group of rare diseases associated with early and transient inflammation vascular injury, followed by fibrosis affecting the skin multiple internal organs. Fibroblast activation hallmark scleroderma disrupting intracellular TGFβ signaling may provide novel approach to controlling fibrosis. Because its potential role in modulating inflammatory fibrotic responses, both PPARγ CB 2 receptors represent attractive targets for development cannabinoid-based therapies. We...
Background andObjectives: Neuroinflammation is associated with many neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). In this study, we investigate the anti-inflammatory, anti-oxidant, and anti-apoptotic properties of two non-psychoactive phytocannabinoids, cannabigerol (CBG) cannabidiol (CBD). Materials Methods: The motoneuron-like cell line NSC-34 differentiated by serum deprivation additional treatment all-trans retinoic acid (RA) a valid model to molecular events...
Abstract Cannabinoids have shown to exert neuroprotective actions in animal models by acting at different targets including canonical cannabinoid receptors and PPARγ. We previously showed that VCE-003, a cannabigerol (CBG) quinone derivative, is novel anti-inflammatory through now generated non-thiophilic VCE-003 derivative named VCE-003.2 preserves the ability activate PPARγ analyzed its activity. This compound exerted prosurvival action progenitor cells during neuronal differentiation,...
Background and Purpose The mechanism of the anti‐migraine action extracts butterbur [ Petasites hybridus (L.) Gaertn.] is unknown. Here, we investigated ability isopetasin, a major constituent these extracts, to specifically target TRPA1 channel affect functional responses relevant migraine. Experimental Approach Single‐cell calcium imaging patch‐clamp recordings in human rodent TRPA1‐expressing cells, neurogenic motor isolated urinary bladder, release CGRP from mouse spinal cord vitro...
Human Gingival Mesenchymal Stem Cells (hGMSC) are multipotential cells that can expand and differentiate in culture under specific standardized conditions. In the present study, we have investigated whether vitro pre-treatment of hGMCs with Cannabidiol (CBD) influence their expression profile, improving therapeutic potential this cell culture. Following CBD treatment (5μM) for 24 h, gene analysis through Next Generation Sequencing (NGS) has revealed several genes differentially expressed...
The cis-stereoisomers of Δ9-THC [(-)-3 and (+)-3] were identified quantified in a series low-THC-containing varieties Cannabis sativa registered Europe as fiber hemp research accessions cannabis. While Δ9-cis-THC (3) occurs cannabis the concentration range (-)-Δ9-trans-THC [(-)-1], it was undetectable sample high-THC-containing medicinal Natural is scalemic (ca. 80-90% enantiomeric purity), absolute configuration major enantiomer established 6aS,10aR [(-)-3] by chiral chromatographic...
The selective inhibition of prostaglandin (PG)E(2) formation via interference with microsomal PGE(2) synthase (mPGES)-1 could have advantages in the treatment PGE(2)-associated diseases, such as inflammation, fever and pain, compared a general suppression all PG biosynthesis, provided by cyclooxygenase (COX)-1 2. Here, we addressed whether naturally occurring acylphloroglucinol myrtucommulone (MC) from Myrtus communis L. (myrtle) affected mPGES-1.The effect MC on was investigated cell-free...
The waxy fraction from the variety Carma of fiber hemp (Cannabis sativa) afforded unusual cannabinoid 4, identified as farnesyl prenylogue cannabigerol (CBG, 1) on basis its spectroscopic properties. A comparative study profile 4 and 1 toward metabotropic (CB1, CB2) ionotropic (TRPV1, TRPV2, TRPM8, TRPA1) targets phytocannabinoids showed that prenylogation increased potency CB2 by ca. 5-fold, with no substantial difference other end-points, except for a decreased affinity TRPM8. isolation...
During a large-scale isolation campaign for the heterodimeric phloroglucinyl pyrone arzanol (1a) from Helichrysum italicum subsp. microphyllum, several new phenolics as well an unusual class of lipids named santinols (5a–c, 6–8) have been characterized. Santinols are angeloylated glycerides characterized by presence branched acyl- or keto-acyl chains and represent hitherto unreported plant lipids. The antibacterial activity selection that includes coumarates, benzofurans, pyrones,...
The present study was designed to investigate the efficacy of a new formulation alone, purified cannabidiol (CBD) (>98 %), main non-psychotropic cannabinoid Cannabis sativa, as topical treatment in an experimental model autoimmune encephalomyelitis (EAE), most commonly used for multiple sclerosis (MS). Particularly, we evaluated whether administration 1 % CBD-cream, given at time symptomatic disease onset, could affect EAE progression and if this also recover paralysis hind limbs, qualifying...
ABSTRACT Research in recent years has extensively investigated the therapeutic efficacy of mesenchymal stromal cells regenerative medicine for many neurodegenerative diseases at preclinical and clinical stages. However, success rate stem cell therapy remains less translational phase. Lack relevant animal models that potentially simulate molecular etiology human pathological symptoms might be a reason behind such poor outcomes associated with therapy. Apparently, self‐renewal differentiation...
Research in the last decades has widely investigated anti-oxidant properties of natural products as a therapeutic approach for prevention and treatment oxidative-stress related disorders. In this context, several studies were aimed to evaluate potential phytocannabinoids, bioactive compounds Cannabis sativa. Here, we examined ability Cannabigerol (CBG), non-psychotropic cannabinoid, still little known, into counteracting hydrogen peroxide (H2O2)-induced oxidative stress murine RAW264.7...
Spurred by a growing interest in cannabidiolquinone (CBDQ, HU-313, 2) as degradation marker and alledged hepatotoxic metabolite of cannabidiol (CBD, 1), we performed systematic study on the oxidation CBD (1) to CBDQ (2) under variety experimental conditions (base-catalyzed aerobic oxidation, with metals, hypervalent iodine reagents). The best results terms reproducibility scalability were obtained λ5-periodinanes (Dess-Martin periodinane, 1-hydroxy-1λ5,2-benziodoxole-1,3-dione (IBX), SIBX,...