A5024108582- Neuroinflammation and Neurodegeneration Mechanisms
- Immunotherapy and Immune Responses
- Neuroscience and Neuropharmacology Research
- Cutaneous Melanoma Detection and Management
- Cancer Immunotherapy and Biomarkers
- Alzheimer's disease research and treatments
- AI in cancer detection
- Immune cells in cancer
- Adenosine and Purinergic Signaling
- Cell Image Analysis Techniques
- Ion Channels and Receptors
- Neurogenesis and neuroplasticity mechanisms
- Melanoma and MAPK Pathways
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Eosinophilic Disorders and Syndromes
- Neurological Disease Mechanisms and Treatments
- T-cell and B-cell Immunology
- melanin and skin pigmentation
- Neuroendocrine Tumor Research Advances
- Neurological Complications and Syndromes
- Plant Toxicity and Pharmacological Properties
- Menstrual Health and Disorders
- Protein Degradation and Inhibitors
- Ocular Oncology and Treatments
University of Florence
2017-2025
Department of Medical Sciences
2024
Pathological Society
2021
Background and Purpose The mechanism of the anti‐migraine action extracts butterbur [ Petasites hybridus (L.) Gaertn.] is unknown. Here, we investigated ability isopetasin, a major constituent these extracts, to specifically target TRPA1 channel affect functional responses relevant migraine. Experimental Approach Single‐cell calcium imaging patch‐clamp recordings in human rodent TRPA1‐expressing cells, neurogenic motor isolated urinary bladder, release CGRP from mouse spinal cord vitro...
Increasing incidence of skin cancer combined with a shortage dermatopathologists have increased the workload pathology departments worldwide. In addition, high intra- and inter-observer variability in assessment melanocytic lesions can result under- or over- estimated diagnosis melanoma. Thus, development new techniques for tumor is essential to assist pathologists standardize diagnoses plan accurate patient treatment. Here, we describe an artificial intelligence (AI) system that recognizes...
Ischemia is a multifactorial pathology characterized by different events evolving in time. Immediately after the ischemic insult, primary brain damage due to massive increase of extracellular glutamate. Adenosine increases dramatically during ischemia concentrations able stimulate all its receptors, A1, A2A, A2B and A3. Although adenosine exerts clear neuroprotective effects through A1 receptors ischemia, use selective receptor agonists hampered their undesirable peripheral side effects. So...
We investigated the different patterns of neurodegeneration and glia activation in CA1 CA3 hippocampal areas TgCRND8 mice. The main feature this transgenic model is rapid development amyloid pathology, which starts already at 3 months age. performed immunohistochemical analyses to compare sensibility two regions neurodegeneration. qualitative quantitative evaluations by fluorescence immunohistochemistry with double or triple staining, followed confocal microscopy digital image analysis...
The biological and clinical relevance of the DNA methylation landscape in metastatic melanoma (MM) remains underexplored. In a retrospective cohort 191 MM lesions from 165 AJCC Stage III IV patients (EPICA cohort) we identified four tumor subsets (i.e. DEMethylated, LOW, INTermediate CIMP) with progressively increasing levels methylation. These findings were validated TCGA MM. EPICA, LOW tumors exhibited significantly longer survival lower progression rate to more advanced stages, compared...
The hippocampus is negatively affected by aging and neurodegenerative diseases leading to impaired learning memory abilities. A diverse series of progressive modifications in the intercellular communication among neurons, astrocytes microglia occur during or inflammation. detailed understanding neurobiological that contribute hippocampal dysfunction may reveal new targets for therapeutic intervention. current study focussed on interplay between neurons astroglia Granule Layer (GL)...
Aging and neurodegenerative diseases share a condition of neuroinflammation entailing the production endogenous cell debris in CNS that must be removed by microglia (i.e., resident macrophages), to restore tissue homeostasis. In this context, extension microglial branches toward underlies mechanisms migration phagocytosis. Amoeboid morphology consequent loss branch functionality characterizes dysregulated microglia. Microglial is assisted another glial population, astroglia, which forms...
Cerebral ischemia is a multifactorial pathology characterized first by an acute injury, due to excitotoxicity, followed secondary brain injury that develops hours days after ischemia. During ischemia, adenosine acts as endogenous neuroprotectant. Few studies have investigated the role of A 2B receptor in because low potency for it and few selective ligands developed so far. receptors are scarcely but widely distributed on neurons, glial endothelial cells hematopoietic cells, lymphocytes...
Tumor microenvironment plays a crucial role in primary cutaneous melanoma (CM) progression. Although the of tumor-infiltrating lymphocyte (TIL) density has been known for long time, its spatial distribution and impact with or without tumor-associated macrophages (TAMs) remain controversial. Herein, we investigated proximity between tumor cells immune 113 CM correlation disease-free (DFS) overall survival (OS). The study cohort included clinical stage II (n = 79) III 34) Breslow thickness >2...
Macrophages (MΦs) and reactive oxygen species (ROS) are implicated in carcinogenesis. The oxidative stress sensor, transient receptor potential ankyrin 1 (TRPA1), activated by ROS, appears to contribute lung breast cancer progression. Although TRPA1 expression has been reported melanoma cell lines, associated with melanocytic transformation, their role remains poorly known. Here, we localized MΦs, the final end-product of stress, 4-hydroxynonenal (4-HNE), tissue samples human common dermal...
The tumor microenvironment (TME) plays a crucial role in melanoma development, progression and response to treatment. As many of the most relevant TME cell phenotypes are defined by simultaneous detection more than two markers, bright-field (BF) multiplex immunohistochemistry (IHC) technique has been introduced for quantitative assessment evaluation relative spatial distances between immune cells cells. In current study, we aimed validate BF IHC techniques Ventana Discovery Ultra...
The presence of tumor-infiltrating lymphocytes (TIL) has been associated with a favorable prognosis primary melanoma (PM). recent development the artificial intelligence (AI) based approach in digital pathology proposed for standardized assessment TIL on hematoxylin and eosin (H&E)-stained images (whole slide images, WSI). Here, we have applied new convolution neural network (CNN) analysis PM WSI to automatically assess infiltration TILs extract score. A CNN was trained validated...
Perineural invasion (PNI), classified according to its presence or absence in tumor specimens, is recognized as a poor prognostic factor pancreatic ductal adenocarcinoma (PDAC) patients. Herein, we identified five histological features of PNI and investigated their impact on survival outcomes PDAC resected Five histopathological (diameter, number, site, sheath involvement, mitotic figures within perineural invasion) were combined an additional final score (ranging from 0 8), clinical data...
Targeting the PD1/PD-L1 immune checkpoint pathway has rapidly become a therapeutic strategy for melanoma patients. Indeed, quantification of PD-L1 expression by immunohistochemistry (IHC) in samples is already required, some contexts, to allow access anti-PD-1/PD-L1 immunotherapy. Despite rising demand testing, paralleling increasing cumulative experience its assessment and quantification, it fair recognize that evaluation still presents critical issues. The aim this technical report develop...
Modifications in the subunit composition of AMPA receptors (AMPARs) have been linked to transition from physiological pathological conditions a number contexts, including EtOH-induced neurotoxicity. Previous work our laboratory showed that EtOH withdrawal causes CA1 pyramidal cell death organotypic hippocampal slices and changes expression AMPARs. Here, we investigated whether function AMPARs may be causal for To this aim, examined composition, localization exposed by using western blotting,...