A5045736692- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Tryptophan and brain disorders
- Cannabis and Cannabinoid Research
- Poisoning and overdose treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Opioid Use Disorder Treatment
- Forensic Toxicology and Drug Analysis
- Photoreceptor and optogenetics research
- Pharmacological Effects and Toxicity Studies
- Memory and Neural Mechanisms
- Epilepsy research and treatment
- Pharmacological Receptor Mechanisms and Effects
- Pharmacovigilance and Adverse Drug Reactions
- Neonatal Health and Biochemistry
- Liver Disease Diagnosis and Treatment
- Migraine and Headache Studies
- Pain Management and Opioid Use
- Autism Spectrum Disorder Research
- Blood Coagulation and Thrombosis Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Heme Oxygenase-1 and Carbon Monoxide
University of Florence
2016-2025
Azienda Ospedaliero-Universitaria Careggi
2015-2024
Emory University
2001-2024
Italian Society of Physiotherapy
2024
Stanford University
2003
Vanderbilt University
2003
Novo Nordisk (Denmark)
1996
University of Perugia
1996
The activation of group I metabotropic glutamate receptors (mGluRs) produces a variety actions that lead to alterations in excitability and synaptic transmission the CA1 region hippocampus. mGluRs, mGluR1 mGluR5, are activated selectively by (<i>S</i>)-3,5-dihydroxyphenylglycine (DHPG). To identify which these mGluR subtypes responsible for various DHPG area CA1, we took advantage two novel subtype-selective antagonists. (<i>S</i>)-(+)-α-amino-a-methylbenzeneacetic acid (LY367385) is potent...
Astrocytes express a wide range of G-protein coupled receptors that trigger release intracellular Ca2+, including P2Y, bradykinin and protease activated (PARs). By using the highly sensitive sniffer-patch technique, we demonstrate activation P2Y receptors, all stimulate glutamate from cultured or acutely dissociated astrocytes. Of these have utilized PAR1 as model system because favourable pharmacological molecular tools, its prominent expression in astrocytes high relevance to...
The serine proteases tissue plasminogen activator, plasmin, and thrombin their receptors have previously been suggested to contribute neuronal damage in certain pathological situations. Here we demonstrate that mice lacking protease-activated receptor 1 (PAR1) a 3.1-fold reduction infarct volume after transient focal cerebral ischemia. Intracerebroventricular injection of PAR1 antagonist BMS-200261 reduced 2.7-fold. There are no detectable differences between -/- WT cerebrovascular anatomy,...
Abstract Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding mechanisms underlying this pathology improve our approaches to development new drugs. Recently, zebrafish has become an important model for studying epilepsy both in basic research drug discovery. Here, we employed transgenic line with pan-neuronal expression genetically-encoded calcium indicator GCaMP6s measure larvae induced by pentylenetretrazole (PTZ). With...
We measured the effects of agonists and antagonists metabotropic glutamate (mGlu) receptors (types 1 5) on NMDA‐induced depolarization mouse cortical wedges in order to characterize mGlu receptor type responsible for modulating NMDA responses. also characterized a number agents by measuring [ 3 H]‐inositol phosphate (IP) formation slices BHK cells expressing either or 5 receptors. (S)‐3,5‐dihydroxyphenylglycine (DHPG), an agonist both receptors, at concentrations ranging from – 10 μ M ,...
In mammalian brain, neurons and astrocytes are reported to express various chloride anion channels, but the evidence for functional expression of Ca 2+ -activated channel (CAAC) its molecular identity have been lacking. Here we report electrophysiological CAAC by mouse Bestrophin 1 ( mBest1 ) in brain. Using imaging perforated-patch-clamp analysis, demonstrate that displayed an inward current at holding potential −70 mV was dependent on increase intracellular after G αq -coupled receptor...
Activation of G protein coupled receptor (GPCR) in astrocytes leads to Ca(2+)-dependent glutamate release via Bestrophin 1 (Best1) channel. Whether receptor-mediated from can regulate synaptic plasticity remains be fully understood.We show here that Best1-mediated astrocytic activates the N-methyl-D-aspartate (NMDAR) and modulates NMDAR-dependent plasticity. Our data activation protease-activated (PAR1) hippocampal CA1 elevates concentration at Schaffer collateral-CA1 (SC-CA1) synapses,...
Prenatal exposure to the antiepileptic drug valproic acid (VPA) induces autism spectrum disorder (ASD) in humans and autistic-like behaviors rodents, which makes it a good model study neural underpinnings of ASD. Rats prenatally exposed VPA show profound deficits social domain. The altered behavior displayed by VPA-exposed rats may be due either deficit reward processing or more general inability properly understand respond signals. To address this issue, we performed behavioral,...
MDPHP is a synthetic cathinone (SC) belonging to α-pyrrolidinophenone derivatives. It central nervous system stimulant and may induce hallucinations, paranoia, tachycardia, hypertension, chest pain, rhabdomyolysis. In literature, few cases of intoxication have been reported. the present study, 17 intake were described including analytical findings clinical manifestations. was quantified by liquid chromatography–tandem mass spectrometry in blood (range 1.26-73.30 ng/mL) urine 19.31-8769.64...
Glutamate plays an important role in the regulation of dopamine neuron activity. In particular, glutamatergic input from subthalamic nucleus is thought to provide control over firing patterns. The degeneration neurons substantia nigra pars compacta (SNc) observed Parkinson9s disease (PD) believed be due a complex interplay factors, including oxidative stress and mitochondrial dysfunction. Although glutamate not primary cause cell death PD, there evidence suggesting excessive release onto may...
The diagnosis of hepatic encephalopathy (HE) relies on clinical, neurophysiological, psychometric and laboratory variables. relationships between such tests remain debated. aim this study was to determine the correlates/prognostic value neurophysiological/psychometric abnormalities in patients with cirrhosis. Seventy-two 14 healthy volunteers underwent EEG paper-and-pencil psychometry (PHES). Blood obtained for C reactive protein (CRP), interleukin 6 (IL6), tumor necrosis factor (TNF)α,...
Limited information is available on the brain expression and role of GPR35, a Gi/o coupled receptor activated by kynurenic acid (KYNA). In mouse cultured astrocytes, we detected GPR35 transcript using RT-PCR found that KYNA (0.1 to 100 µM) decreased forskolin (FRSK)-induced cAMP production (p<0.05). Both CID2745687 (3 µM, CID), recently described antagonist, gene silencing significantly prevented action FRSK-induced production. these cultures, then evaluated whether activation was able...
Abstract Idiopathic pulmonary fibrosis is a severe disease characterized by excessive myofibroblast proliferation, extracellular matrix and fibrils deposition, remodelling of lung parenchyma insufficiency. Drugs able to reduce progression are available, but therapeutic results unsatisfactory; new safe treatments urgently needed. Poly(ADP‐ribose) polymerases‐1 ( PARP ‐1) an abundant nuclear enzyme involved in key biological processes: DNA repair, gene expression control, cell survival or...
A prominent role of hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels has been suggested based on their expression and (dys)function in dorsal root ganglion (DRG) neurons, being likely involved peripheral nociception. Using HCN blockers as antinociceptive drugs is prevented by the widespread distribution these channels. However, tissue-specific isoforms varies significantly, HCN1 HCN2 considered major players DRG excitability. We characterized pharmacological effect a novel...
Our consensus statement aims to clarify the use of antidepressants and anxiolytics during breastfeeding amidst clinical uncertainty. Despite recent studies, potential harm breastfed newborns from these medications remains a concern, leading abrupt discontinuation necessary treatments or exclusive formula feeding, depriving benefits mother's milk.
Vascular endothelial growth factor (VEGF)-A is a main player in the development of neuropathic pain induced by chemotherapy and pharmacological blockade VEGF receptor (VEGFR) subtype 1 killer strategy. Interestingly, VEGF-A has been demonstrated to have also neuroprotective properties. The aim study was investigate role against oxaliplatin neurotoxicity, attempting discriminate pain-related restorative signaling pathways. We used rat organotypic spinal cord slices treated with oxaliplatin,...
Abstract Synthetic cathinones (SCs) were confirmed as the second most prevalent class of NPS in 2024, underscoring their widespread availability and use. Notably, SCs seizure related intoxication cases increased within European Union, involving mostly 3-chloromethcathinone, 3-methylmethcathinone, 2-methylmethcathinone, N-ethylnorpentedrone. Italy has observed a distinct trend, with methylendioxy pyrrolidinohexanophenone (MDPHP) emerging significant concern. This technical note aims to...