A5041161550- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Nicotinic Acetylcholine Receptors Study
- Epilepsy research and treatment
- Molecular Sensors and Ion Detection
- Adenosine and Purinergic Signaling
- Genomics and Rare Diseases
- Chemical Synthesis and Analysis
- Neural dynamics and brain function
- RNA and protein synthesis mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Amino Acid Enzymes and Metabolism
- Pharmacological Receptor Mechanisms and Effects
- Protease and Inhibitor Mechanisms
- Neurological disorders and treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Memory and Neural Mechanisms
- Genomic variations and chromosomal abnormalities
- Neuropeptides and Animal Physiology
- RNA regulation and disease
Emory University
2016-2025
NeurOp (United States)
2024
Center for Cancer and Blood Disorders
2022
National University of Singapore
2003-2021
University of Montana
2014-2021
University of Colorado Denver
2021
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2010-2021
National Institutes of Health
2016-2021
Northwestern University
2021
MRC Laboratory of Molecular Biology
2021
Modulation of postsynaptic AMPA receptors in the brain by phosphorylation may play a role expression synaptic plasticity at central excitatory synapses. It is known from biochemical studies that GluR1 receptor subunits can be phosphorylated within their C terminal cAMP-dependent protein kinase A (PKA), which colocalized with phosphatase calcineurin (i.e., 2B). We have examined effect PKA and on time course, peak open probability ( P O,PEAK ), single-channel properties glutamateevoked...
1. The CA1 region of rat hippocampal slices bathed in 8.5 mM interstitial K+ ([K+]o) exhibited spontaneous 20- to 90-s electrographic seizures at regular intervals 1–8 min. In these same CA3 neurons generated interictal bursts that propagated throughout the pyramidal cell subfields. contained components reminiscent discharges recorded vivo during tonic-clonic motor seizures. tonic phase lasted 1–10 s, consisted a sustained depolarization and firing cells, was associated with negative...
The activation of group I metabotropic glutamate receptors (mGluRs) produces a variety actions that lead to alterations in excitability and synaptic transmission the CA1 region hippocampus. mGluRs, mGluR1 mGluR5, are activated selectively by (<i>S</i>)-3,5-dihydroxyphenylglycine (DHPG). To identify which these mGluR subtypes responsible for various DHPG area CA1, we took advantage two novel subtype-selective antagonists. (<i>S</i>)-(+)-α-amino-a-methylbenzeneacetic acid (LY367385) is potent...
NR2A and NR2B are the predominant NR2 NMDA receptor subunits expressed in cortex hippocampus. The relative expression level of is regulated developmentally these two have been suggested to play distinct roles long-term synaptic plasticity. We used patch-clamp recording recombinant receptors HEK293 cells characterize activation properties both NR1/NR2A NR1/NR2B receptors. Recordings from outside-out patches that contain a single active channel show NR2A-containing higher probability opening...
The function of the N-methyl-D-aspartate (NMDA)-preferring glutamate receptor can be regulated by extracellular pH, a process that may important during ischemia in brain or seizures. Protons inhibit NMDA 50 percent at pH 7.3 through interactions with NR1 subunit, and both polyamines exon 5 potentiate relief tonic proton inhibition present physiological pH. A single amino acid (lysine 211) was identified mediates effects rat brain. Electroneutral substitutions this position restored...
Objective Early-onset epileptic encephalopathies have been associated with de novo mutations of numerous ion channel genes. We employed techniques modern translational medicine to identify a disease-causing mutation, analyze its altered behavior, and screen for therapeutic compounds treat the proband. Methods Three tools were utilized: (1) high-throughput sequencing technology novel mutation; (2) in vitro expression electrophysiology assays confirm variant protein's dysfunction; (3)...
<h3>Background</h3> We aimed for a comprehensive delineation of genetic, functional and phenotypic aspects <i>GRIN2B</i> encephalopathy explored potential prospects personalised medicine. <h3>Methods</h3> Data 48 individuals with de novo variants were collected from several diagnostic research cohorts, as well 43 patients the literature. Functional consequences response to memantine treatment investigated in vitro eventually translated into patient care. <h3>Results</h3> Overall, 86...
The factors responsible for the unusual susceptibility of hippocampus to seizures and ischemic cell damage are not well understood. CA1 pyramidal subfield is particularly vulnerable seizure activity after ischemia. possibility was examined that regional differences exist in extracellular volume, which might influence neuronal excitability response injury hippocampus. stratum pyramidale exhibited an exceptionally low volume fraction (EVF) 0.12, whereas EVFs CA3 dentate were considerably...
Zinc inhibits NMDA receptor function through both voltage-dependent and voltage-independent mechanisms. In this report we have investigated the role that NR1 subunit plays in Zn2+ inhibition. Our data show inclusion of exon 5 into increases IC50 for inhibition from 3-fold to 10-fold when full length 22 is also spliced mature transcript complex contains NR2A or NR2B subunits; has little effect on receptors contain NR2C NR2D. Mutagenesis within indicates same residues control proton...