A5074430495- Ion Channels and Receptors
- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- Pediatric Pain Management Techniques
- Herbal Medicine Research Studies
- Endometriosis Research and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
- Neuropeptides and Animal Physiology
- Biochemical effects in animals
- Menstrual Health and Disorders
- Circadian rhythm and melatonin
- Pain Management and Opioid Use
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Migraine and Headache Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Phytochemicals and Antioxidant Activities
- melanin and skin pigmentation
- Perfectionism, Procrastination, Anxiety Studies
- Adenosine and Purinergic Signaling
- Dermatology and Skin Diseases
- Atherosclerosis and Cardiovascular Diseases
University of Florence
2019-2025
University of Siena
2024
ORCID
2021
Universidade Federal Fluminense
2016-2020
It is known that transient receptor potential ankyrin 1 (TRPA1) channels, expressed by nociceptors, contribute to neuropathic pain. Here we show TRPA1 also in Schwann cells. We found mice with partial sciatic nerve ligation, silencing nociceptors attenuated mechanical allodynia, without affecting macrophage infiltration and oxidative stress, whereas cells reduced both allodynia neuroinflammation. Activation of cell evoked NADPH oxidase (NOX1)-dependent H2O2 release, or blocking NOX1...
Abstract Efficacy of monoclonal antibodies against calcitonin gene-related peptide (CGRP) or its receptor (calcitonin receptor-like receptor/receptor activity modifying protein-1, CLR/RAMP1) implicates peripherally-released CGRP in migraine pain. However, the site and mechanism CGRP-evoked peripheral pain remain unclear. By cell-selective RAMP1 gene deletion, we reveal that released from mouse cutaneous trigeminal fibers targets CLR/RAMP1 on surrounding Schwann cells to evoke periorbital...
Excessive alcohol consumption is associated with spontaneous burning pain, hyperalgesia, and allodynia. Although acetaldehyde has been implicated in the painful alcoholic neuropathy, mechanism by which ethanol metabolite causes pain symptoms unknown. Acute ingestion caused delayed mechanical allodynia mice. Inhibition of dehydrogenase (ADH) or deletion transient receptor potential ankyrin 1 (TRPA1), a sensor for oxidative carbonyl stress, prevented Acetaldehyde generated ADH both liver...
Abstract Although macrophages (MΦ) are known to play a central role in neuropathic pain, their contribution cancer pain has not been established. Here we report that depletion of sciatic nerve resident MΦs (rMΦ) mice attenuates mechanical/cold hypersensitivity and spontaneous evoked by intraplantar injection melanoma or lung carcinoma cells. MΦ-colony stimulating factor (M-CSF) was upregulated the trunk mediated cancer-evoked via rMΦ expansion, transient receptor potential ankyrin 1 (TRPA1)...
Insulin growth factor-1 (IGF-1), an osteoclast-dependent osteolysis biomarker, contributes to metastatic bone cancer pain (MBCP), but the underlying mechanism is poorly understood. In mice, femur metastasis caused by intramammary inoculation of breast cells resulted in IGF-1 increase and sciatic nerve, IGF-1-dependent stimulus/non-stimulus-evoked pain-like behaviors. Adeno-associated virus-based shRNA selective silencing receptor (IGF-1R) Schwann cells, not dorsal root ganglion (DRG)...
Abstract Oxidative stress is implicated in retinal cell injury associated with glaucoma and other diseases. However, the mechanism by which oxidative leads to damage not completely understood. Transient receptor potential ankyrin 1 (TRPA1) a redox-sensitive channel that, amplifying signal, promotes inflammation tissue injury. Here, we investigated role of TRPA1 evoked ischemia (1 hour) reperfusion (I/R) mice. In wild-type mice, numbers thickness were reduced at both day-2 day-7 after I/R. By...
Ingestion of alcoholic beverages is a known trigger migraine attacks. However, whether and how ethanol exerts its pro-migraine action remains poorly known. Ethanol stimulates the transient receptor potential vanilloid 1 (TRPV1) channel, dehydrogenized metabolite, acetaldehyde, TRP ankyrin (TRPA1) agonist.Periorbital mechanical allodynia following systemic acetaldehyde was investigated in mice after TRPA1 TRPV1 pharmacological antagonism global genetic deletion. Mice with selective silencing...
Abstract Background The mechanism underlying the pain symptoms associated with chemotherapeutic-induced peripheral neuropathy (CIPN) is poorly understood. Transient receptor potential ankyrin 1 (TRPA1), TRP vanilloid 4 (TRPV4), TRPV1, and oxidative stress have been implicated in several rodent models of CIPN-evoked allodynia. Thalidomide causes a painful CIPN patients via an unknown mechanism. Surprisingly, pathway responsible for such proalgesic response has not yet investigated animal...
Background and Purpose Fibromyalgia is a complex clinical disorder with an unknown aetiology, characterized by generalized pain co‐morbid symptoms such as anxiety depression. An imbalance of oxidants antioxidants proposed to play pivotal role in the pathogenesis fibromyalgia symptoms. However, precise mechanisms which oxidative stress contributes fibromyalgia‐induced remain unclear. The transient receptor potential ankyrin 1 (TRPA1) channel, known both sensor sensor, has been implicated...
Macrophages (MΦs) and reactive oxygen species (ROS) are implicated in carcinogenesis. The oxidative stress sensor, transient receptor potential ankyrin 1 (TRPA1), activated by ROS, appears to contribute lung breast cancer progression. Although TRPA1 expression has been reported melanoma cell lines, associated with melanocytic transformation, their role remains poorly known. Here, we localized MΦs, the final end-product of stress, 4-hydroxynonenal (4-HNE), tissue samples human common dermal...
Background and Purpose The pro‐algesic transient receptor potential ankyrin 1 (TRPA1) channel, expressed by a subpopulation of primary sensory neurons, has been implicated in various pain models mice. However, evidence rats indicates that TRPA1 conveys nociceptive signals elicited channel activators, but not those associated with tissue inflammation or nerve injury. Here, rats, we explored the role mechanical allodynia stimulation peptidergic neurons (neurogenic inflammation) moderate...
Over 60% of women with endometriosis experience abdominopelvic pain and broader manifestations, including chronic back pain, fibromyalgia, fatigue, vulvodynia, migraine. Although the imbalance proinflammatory mediators, complement component C5a, is associated endometriosis-related mechanisms causing widespread C5a role remain unclear. Female mice exhibit increased plasma levels pain. We hypothesize Schwann cells involvement in endometriotic Here, we show that silencing receptor (C5aR1)...
Growing evidence indicates that transient receptor potential (TRP) channels contribute to different forms of pruritus. However, the endogenous mediators cause itch through signaling are poorly understood. In this study, we show genetic deletion or pharmacological antagonism TRPV4 attenuated in a mouse model psoriasis induced by topical application imiquimod. Human psoriatic lesions showed increased expression several microRNAs, including miR-203b-3p, which calcium ion response rodent dorsal...
: Transient receptor potential ankyrin 1 (TRPA1) activation is implicated in neuropathic pain-like symptoms. However, whether TRPA1 solely pain-signaling or contributes to neuroinflammation multiple sclerosis (MS) unknown. Here, we evaluated the role underlying symptoms using two different models of MS.
Analgesia by non-steroidal anti-inflammatory drugs (NSAIDs) is ascribed to inhibition of prostaglandin (PG) biosynthesis and ensuing inflammation. However, NSAIDs have life-threatening side effects, inflammation delays pain resolution. Decoupling the mechanisms underlying PG-evoked vs. protective would facilitate treatment. Herein, we reveal that selective silencing PGE2 EP2 receptor in Schwann cells via an adeno-associated viral vector abrogates indomethacin-sensitive component pain-like...