A5045833613- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Fungal and yeast genetics research
- Mitochondrial Function and Pathology
- Plant nutrient uptake and metabolism
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Plant Pathogens and Fungal Diseases
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Plant tissue culture and regeneration
- Diet and metabolism studies
- Muscle Physiology and Disorders
- Genetic Neurodegenerative Diseases
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Electromagnetic Fields and Biological Effects
- Cardiovascular Disease and Adiposity
- RNA and protein synthesis mechanisms
- Plant Disease Resistance and Genetics
- Coenzyme Q10 studies and effects
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Banana Cultivation and Research
- Plant Physiology and Cultivation Studies
University of Urbino
2012-2025
Istituto di Chimica Biomolecolare
1998
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mass membrane potential analysed Mitotracker Green JC-1 stains epifluorescence microscope. Expression of PGC1α, NRF1α, Tfam genes controlling biogenesis was studied real-time PCR. The...
Exercise-released extracellular vesicles (EVs) are emerging as a novel class of exerkines that promotes systemic beneficial effects. However, slight differences in the applied exercise protocols terms mode, intensity and duration, well need for standardized EV isolation, make comparison studies literature extremely difficult. This work aims to investigate amount EV-associated miRNAs released circulation response different physical regimens. Healthy individuals were subjected protocols: acute...
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The alignment of the 28S gene several species Pezizales allowed to select two pairs primers able amplify internal transcribed spacer region ribosomal DNA in mycorrhizal fungi, such as truffles. higher yield amplification product demonstrates a better annealing new rDNA, compared universal 1 and 4. Therefore, can be used an easier more sensitive tool for identification truffle any stage their life cycle, including phase.
This study describes the effects of a static magnetic field (SMF) on cell growth and DNA integrity human umbilical vein endothelial cells (HUVECs). Fast halo assay was used to investigate nuclear damage; quantitative polymerase chain reaction (QPCR), standard PCR, real-time PCR were evaluate mitochondrial integrity, content, gene expression. HUVECs continually exposed 300 mT SMF for 4, 24, 48, 72 h. Compared control samples (unexposed cultures) SMF-exposed did not show statistically...
Abstract In a previous investigation we reported that exposure to moderate (300 mT) static magnetic field (SMF) causes transient DNA damage and promotes mitochondrial biogenesis in human umbilical vein endothelial cells (HUVECs). To better understand the response of HUVECs 300 mT SMF, high‐quality subtracted cDNA library representative genes induced after 4 h was constructed. The global gene expression profile showed several were SMF exposure. characterized clones are involved cell...
The present work aimed to investigate whether exposure static magnetic field (SMF) and extremely low frequency (ELF-MF) can induce biomolecular changes on Tuber borchii hyphal growth. mycelium was exposed for 1 h 3 consecutive days a SMF of 300 mT or an ELF-MF 0.1 50 Hz. Gene expression biochemical analyses were performed. In mycelia ELF-MF, some genes involved in growth, investigated using quantitative real-time polymerase chain reaction, upregulated, the activity many glycolytic enzymes...
Summary • NADP‐glutamate dehydrogenase (NADP‐GDH) from Tuber borchii was purified and the corresponding gene cloned in order to elucidate physiological role of enzyme this ectomycorrhizal fungus. NADP‐GDH using an anion‐exchange column followed by affinity chromatography. The complete a 30‐d‐old‐mycelium cDNA library characterized. T. appears be physically kinetically similar those other fungi deduced amino acid sequence gdh showed significant similarity fungal NADP‐dependent GDHs....
Abstract Background Small GTPases of the Rho family function as tightly regulated molecular switches that govern important cellular functions in eukaryotes. Several families regulatory proteins control their activation cycle and subcellular localization. Members guanine nucleotide dissociation inhibitor (GDI) sequester from plasma membrane keep them an inactive form. Results We report on characterization RhoGDI homolog Tuber borchii Vittad., ascomycetous ectomycorrhizal fungus. The Tb gdi...
During the life cycle of mycorrhizal fungi, morphological, genetic and metabolic modifications are induced in fungus its symbiotic partner. These changes influenced by environmental factors: light, gravity, oxygen, temperature, soil type, nutrients, root exudates presence particular bacterial perhaps fungal viral populations mycorrhizosphere. To determine whether different carbohydrates lead to cell-signalling events morphofunctional cultured Tuber borchii mycelia, expression level genes...