A5038044408- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Trace Elements in Health
- Advanced biosensing and bioanalysis techniques
- Selenium in Biological Systems
- Cultural Heritage Materials Analysis
- Advanced Chemical Sensor Technologies
- Biochemical and Structural Characterization
- Mitochondrial Function and Pathology
- Phytochemicals and Antioxidant Activities
- Biosensors and Analytical Detection
- Fermentation and Sensory Analysis
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Metal complexes synthesis and properties
- Antimicrobial Peptides and Activities
- Drug Transport and Resistance Mechanisms
- Algal biology and biofuel production
- Muscle metabolism and nutrition
- Bacterial Genetics and Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Plant-Microbe Interactions and Immunity
University of Naples Federico II
2016-2025
Istituto Nazionale Biostrutture e Biosistemi
2013-2025
Ceinge Biotecnologie Avanzate (Italy)
1997-2024
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2013-2020
Federico II University Hospital
2011-2020
Naples Anesthesia & Physician Associates
2019
Institute of Biostructure and Bioimaging
2014-2017
University of the Basque Country
2013
Consejo Superior de Investigaciones Científicas
2013
University of Molise
2013
Abstract The solution structure and stability of N‐terminally truncated β2‐microglobulin (δN6β2‐m), the major modification in ex vivo fibrils, have been investigated by a variety biophysical techniques. results show that δN6β2‐m has free energy stabilization is reduced 2.5 kcal/mol compared to intact protein. Hydrogen exchange mixture full‐length proteins at μM concentrations pH 6.5 monitored electrospray mass spectrometry reveals significantly less protected than its wild‐type counterpart....
In this work, we assess the potential of waste products Phlegrean mandarin (Citrus reticulata Blanco), namely seeds and peel, to be reutilized as a source bioactive compounds beneficial for human diet. Starting from evidence that by-products specific cultivar are most powerful sources antioxidants compared pulp, have investigated if how in peel may affected by fruit ripening. Three stages ripening been considered our study: unripe fruits = UF, semi-ripe SRF, ripe RF. The overall results...
The prototypic long pentraxin PTX3 is a unique fluid-phase pattern recognition receptor that plays nonredundant role in innate immunity and female fertility. C-terminal domain required for C1q complement activation contains single N-glycosylation site on Asn 220. In the present study, we characterized structure of human glycosidic moiety investigated its relevance interaction classical pathway. By specific endo exoglycosidases digestion direct mass spectrometric analysis, found both...
In the presence of a bacteriophage (a bacteria-attacking virus) resistance is clearly beneficial to bacteria. As expected in such conditions, resistant bacteria emerge rapidly. However, absence phage, often display reduced fitness, compared their sensitive counterparts. The present study explored fitness cost associated with phage-resistance as an opportunity isolate attenuated strain S. aureus. phage-resistant A172 was isolated from phage-sensitive A170 MSa phage. Acquisition altered...
The different thermal behaviors and solubilities of large structurally polycyclic aromatic hydrocarbon (PAH) mixtures featuring coal tar pitch (CP) naphthalene synthetic (NP) samples could be read in light their molecular weight (MW) distribution spectroscopic features. number-average MW obtained by mass spectrometry for CP (417 Da) NP (691 resulted to lower comparison the values evaluated size-exclusion chromatography (SEC) (796 824 Da NP, respectively) because response detector low-...
Arbuscular mycorrhizal fungi (AMF) are obligate plant biotrophs that may contain endobacteria in their cytoplasm. Genome sequencing of Candidatus Glomeribacter gigasporarum revealed a reduced genome and dependence on the fungal host. RNA-seq analysis AMF Gigaspora margarita presence absence endobacterium indicated have an important role pre-symbiotic phase by enhancing bioenergetic capacity. To improve understanding fungal-endobacterial interactions, iTRAQ (isobaric tags for relative...
Cisplatin (CDDP) can be encapsulated within the central cavity of reconstituted (apo)ferritin, (A)Ft, to form a drug-loaded protein potential great interest for targeted cancer treatments.
Reverse micelle nanoreactors were successfully designed to synthesize small-sized ceria nanocrystals (3.5–4.2 nm) with a sizeable amount of substitutional iron. Undoped and doped CeO2 catalysts an iron content (0.50–10 mol %) compliant the nominal value prepared tested for first time removal ibuprofen both in dark under UV or visible light irradiation. The effective inclusion distribution lattice ascertained through in-depth physicochemical characterization. In particular, X-ray diffraction...
We investigated the effect of growing on lactate instead glucose in human cardiomyocyte assessing their viability, cell cycle activity, oxidative stress and metabolism by a proteomic metabolomic approach. In previous studies performed elite players, we found that adaptation to exercise is characterized chronic high plasma level lactate. Lactate considered not only an energy source but also signalling molecule referred as "lactormone"; heart one major recipients exogenous With this mind, used...
A member of the laccase multigene family in Pleurotus ostreatus has been cloned and sequenced. The gene structure determined by comparison with corresponding cDNA, synthesized reverse transcription/PCR amplification. encode a isoenzyme 533 amino acids which already purified characterized [Palmieri, G., Giardina, P., Marzullo, L., Desiderio, B., Nitti, Cannio, R. & Sannia, G.(1993) Appl. Microbiol. Biotechnol. 39, 632-636]. More than 92% protein sequence, including N C termini, verified...
To assess the psychometric properties of Mobility Section Prosthesis Evaluation Questionnaire (PEQ-MS).A postal survey, including self-report assessment prosthetic capability and performance with PEQ-MS Locomotor Capabilities Index, other variables associated wear use. The data underwent Rasch analysis for rating scale diagnostics a reliability validity study.A total 123 subjects (mean age 54 years) who had undergone lower limb amputation in previous 5 years completed rehabilitation training...
Glioblastoma Multiforme (GBM) is the most common and aggressive human brain tumor, associated with very poor survival despite surgery, radiotherapy chemotherapy.The epidermal growth factor receptor (EGFR) platelet-derived β (PDGFRβ) are hallmarks in GBM driving roles tumor progression. In approximately half of tumors amplified EGFR, EGFRvIII truncated extracellular mutant detected. does not bind ligands, highly oncogenic its expression confers resistance to EGFR tyrosine kinase inhibitors...
Auoxo3, a cytotoxic gold(iii) compound, was encapsulated within ferritin nanocage. Inductively coupled plasma mass spectrometry, circular dichroism, UV-Vis absorption spectroscopy and X-ray crystallography confirm the potential-drug encapsulation. The structure shows that naked Au(i) ions bind to side chains of Cys48, His49, His114, His114 Cys126, His132, His147. gold-encapsulated nanocarrier has effect on different aggressive human cancer cells, whereas it is significantly less for...