A5021932511- Animal Disease Management and Epidemiology
- Cervical Cancer and HPV Research
- Virus-based gene therapy research
- Veterinary Oncology Research
- Microbial infections and disease research
- Bladder and Urothelial Cancer Treatments
- Animal Virus Infections Studies
- Autophagy in Disease and Therapy
- Molecular Biology Techniques and Applications
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Cancer Research and Treatments
- T-cell and Retrovirus Studies
- Brucella: diagnosis, epidemiology, treatment
- Ubiquitin and proteasome pathways
- Tuberculosis Research and Epidemiology
- Urological Disorders and Treatments
- Bacteriophages and microbial interactions
- Heat shock proteins research
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- Viral gastroenteritis research and epidemiology
- Cancer, Hypoxia, and Metabolism
- Animal health and immunology
- Milk Quality and Mastitis in Dairy Cows
University of Naples Federico II
2015-2024
Iuliu Hațieganu University of Medicine and Pharmacy
2022
University of Bari Aldo Moro
2009-2020
Federico II University Hospital
2010-2011
University of Veterinary Medicine
2003
In the presence of a bacteriophage (a bacteria-attacking virus) resistance is clearly beneficial to bacteria. As expected in such conditions, resistant bacteria emerge rapidly. However, absence phage, often display reduced fitness, compared their sensitive counterparts. The present study explored fitness cost associated with phage-resistance as an opportunity isolate attenuated strain S. aureus. phage-resistant A172 was isolated from phage-sensitive A170 MSa phage. Acquisition altered...
Bovine papillomavirus type 2 (BPV-2) infection has been associated with urinary bladder tumours in adult cattle grazing on bracken fern-infested land. In this study, we investigated the simultaneous presence of BPV-2 whole blood and an attempt to better understand biological role circulating BPV-2. Peripheral samples were collected from 78 clinically suffering a severe chronic enzootic haematuria. Circulating DNA was detected 61 them two healthy cows. Fifty affected animals slaughtered at...
Bovine papillomavirus type 2 (BPV-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the Marmara Black Sea Regions Turkey. BPV-2 DNA found both neoplastic non-neoplastic lesions bladder. Furthermore, this virus may be normal inhabitant since also detected clinically buffaloes. The viral activation by fern immunosuppressant or carcinogen trigger urothelial cell transformation. E5 oncoprotein was solely tumours appeared...
Papillomaviruses (PVs) are believed to be highly epitheliotropic as they usually establish productive infections within stratified epithelia. In vitro, various PVs appear complete their entire life-cycle in different trophoblastic cell lines. this study, infection by and protein expression of bovine papillomavirus type 2 (BPV-2) the uterine chorionic epithelium placenta has been described four cows suffering from naturally occurring papillomavirus-associated urothelial bladder tumors. E5...
Equine sarcoids are caused by infection with bovine papillomavirus (BPV) types 1, 2, and possibly 13. However, a number of lack BPV DNA, new potential etiological agents for sarcoid diseases need to be considered. High-performance digital droplet polymerase chain reaction (ddPCR) was used the quantitative detection ovine (OaPV) 1–4 DNA from 63 samples collected in Austria. All were comparatively evaluated OaPV loads qPCR. Conventional PCR amplicon sequencing validate data. Of isolates, ddPCR...
Samples of neoplastic and normal urothelium were obtained from cows originating areas southern Italy, a region in which chronic enzootic haematuria is endemic bracken fern infestation widespread. Specimens analysed for bovine papillomavirus type 2 (BPV-2) DNA, BPV-2 E5 expression telomerase activity. A total 46 60 tumours 17 34 bladder mucosa samples harboured DNA. Analysis subset showed protein activity tumour tissue only. No positive BPV DNA or activity, suggesting the presence latent...
Bovine papillomavirus type 2 (BPV-2) is an oncogenic virus infecting both epithelial and mesenchymal cells. Its life cycle, similar to other papillomaviruses (PVs), appears be linked differentiation. Human bovine PVs have been known reside in a latent, episomal form PBMCs; therefore, it believed that blood cells, like all function as non-permissive carriers. Here, for the first time veterinary comparative medicine, BPV-2 E5 oncoprotein major structural L1 capsid protein, expressed only...
Bovine papillomavirus type 13 (BPV-13), a novel Deltapapillomavirus, has been found associated with urothelial tumours of the urinary bladder cattle grazing on lands infested bracken fern. BPV-13 was detected in 28 39 tumours. Diagnosis based sequencing L1 and E5 amplicons from tumour samples. The nucleotide sequences generated these showed 100% homology DNA Brazil fibropapilloma ear cow equine sarcoids two horses. GenBank accession number our representative is JQ798171.1. Furthermore, mRNA...
Background Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of contaminants in water as well solid matrices. Although important be detected, small analytes (few hundreds Daltons) are an issue biosensing since signal they induce transducer, specifically Quartz-Crystal Microbalance, is undetectable. A pesticide like parathion (M = 292 Da) typical example contaminant which amplification procedure desirable....
ABSTRACT Brucellosis is a costly disease of water buffaloes ( Bubalus bubalis ). Latent infections and prolonged incubation the pathogen limit efficacy programs based on eradication infected animals. We exploited genetic selection for resistance as an approach to control buffalo brucellosis. tested 231 cows presence anti- Brucella abortus antibodies (by agglutination complement fixation tests) Nramp1 genotype PCR-denaturing gradient gel electrophoresis). When animals (58 cases 173 controls)...
Journal Article Heterogeneous shedding of Brucella abortus in milk and its effect on the control animal brucellosis Get access R. Capparelli, Capparelli Faculty Biotechnological Sciences, University Naples "Federico II", Naples, Italy Search for other works by this author on: Oxford Academic Google Scholar M. Parlato, Parlato Agriculture, Iannaccone, Iannaccone S. Roperto, Roperto Veterinary Medicine, Marabelli, Marabelli Department Public Health, Nutrition Food Safety, Ministry Rome, F. D....
Background Calpain 3 (Capn3), also named p94, is a skeletal muscle tissue-specific protein known to be responsible for limb-girdle muscular dystrophy type 2A (LGMD2A). Recent experimental studies have hypothesized pro-apoptotic role of Capn3 in some melanoma cell lines. So far the link between calpain3 and tumors comes from vitro studies. The objective this study was describe activation naturally occurring urothelial urinary bladder cattle. Methods Findings Here we describe, first time...
Background Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, anogenital tract and oral cavity. In this study, early (E) late (L) protein expression bovine papillomavirus type 2 (BPV-2) in urothelium urinary bladder is described cows water buffaloes suffering from naturally occurring papillomavirus-associated urothelial tumors. Methods Findings E5 protein, major oncoprotein BPV-2, was detected all L1 DNA...
The serum- and glucocorticoid-regulated kinase (SGK1) controls cell transformation tumor progression. SGK1 affects mitotic stability by regulating the expression of RANBP1/RAN. Here, we demonstrate that fluctuations indirectly modify maturation pre-miRNAs, modulating equilibrium RAN/RANBP1/RANGAP1 axis, main regulator nucleo-cytoplasmic transport. levels pre-miRNAs mature miRNAs were assessed qRT-PCR, in total extracts after differential nuclear/cytoplasmic extraction. RANBP1 is limiting...